- Clone
- RMG2a-62 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Immunoglobulin G2a
- Isotype
- Rat IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![a-RMG2a-62_AF647_IgG2a_Antibody_FC_041714.jpg a-RMG2a-62_AF647_IgG2a_Antibody_FC_041714.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RMG2a-62_AF647_IgG2a_Antibody_FC_041714.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with purified anti-human CD3 (clone HIT3a, mouse IgG2a isotype, filled histogram) or mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG2a (clone RMG2a-62) Alexa Fluor® 647. -
IHC staining of Alexa Fluor® 647 anti-mouse IgG2a on formalin-fixed paraffin-embedded human tonsil. Following antigen retrieval using Tris-EDTA, pH 9.0, the tissue was incubated with 5 µg/mL of purified mouse anti-CD45RO (Cat. No. 304202) at 4°C. This was followed by incubation with Alexa Fluor® 647 goat anti-mouse IgG2a (clone RMG2a-62) at 10 µg/mL. Nuclei were counterstained with DAPI (blue, panel A) (Cat. No. 422801) and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40X objective. Scale: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
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407115 | 25 µg | $89 | ||||
407116 | 100 µg | $194 |
The RMG2a-62 monoclonal antibody reacts with immunoglobulin G2a and G2c (IgG2a, IgG2c) and in all tested mouse haplotype (Igh-a and b). It does not react with other isotypes. The RMG2a-62 monoclonal antibody may be used as primary or secondary reagent for ELISA or immunofluorescent analysis.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Mouse Ig cocktail
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
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Application References
(PubMed link indicates BioLegend citation) -
- Gavin AL, et al.2006. Science 314:1936.
- Podolin PL, et al. 2008. J. Immunol. 180:7989. (IP) PubMed
- Product Citations
- RRID
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AB_2563482 (BioLegend Cat. No. 407115)
AB_2563483 (BioLegend Cat. No. 407116)
Related FAQs
- IgG2a gene is deleted in some mouse strains, which ones are they?
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IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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