- Clone
- Poly4053 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Goat Polyclonal IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![Poly4053_A647_IgG_Antibody_1_111120 Poly4053_A647_IgG_Antibody_1_111120](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/Poly4053_A647_IgG_Antibody_1_111120.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Tris-Buffered Saline 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/ml of purified anti-human CD309/VEGFR2 (clone A16085I) and Alexa Fluor® 594 anti-human PNAD (clone MECA-79) (green) at 4°C overnight, followed by 2.5 µg/ml of Alexa Fluor® 647 anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The image was captured with a 10X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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405322 | 100 µg | $183 |
This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Polyclonal
- Host Species
- Goat
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 2.5 - 5.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.
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Application References
(PubMed link indicates BioLegend citation) -
- Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
- Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
- Winters T, et al. 2014. EMBO J. 33:1256-70.
- Product Citations
- RRID
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AB_2563045 (BioLegend Cat. No. 405322)
Antigen Details
- Distribution
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B cells
- Gene ID
- 16059 View all products for this Gene ID
- UniProt
- View information about IgG on UniProt.org
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