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Alexa Fluor® 700 anti-mouse Ly-6C Antibody

Pricing & Availability
Clone
HK1.4 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus C
Isotype
Rat IgG2c, κ
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Product Citations
publications
HK1dot4_AF700_072210
C57BL/6 bone marrow cells stained with HK1.4 Alexa Fluor® 700
  • HK1dot4_AF700_072210
    C57BL/6 bone marrow cells stained with HK1.4 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
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128023 25 µg $124
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128024 100 µg $265
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Description

Most hematopoietic cells express one or more members of Ly-6 family. The expression of Ly-6 varies with development stage and activation. Ly-6C is a 14-17 kD GPI-linked surface protein expressed on mouse monocyte/macrophage cells, endothelial cells, neutrophils, and some T cell subsets. Ly-6C is reported to be an indicator of memory CD8+ T cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
L3 cloned CTL cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Clone HK1.4 does not block the binding of clone RB6-8C58.

Additional reported applications (for relevant formats of this clone) include: in vitro activation of T cells1-3 and immunohistochemistry of frozen sections4.

Application References

(PubMed link indicates BioLegend citation)
  1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (Activ)
  2. Herold KC, et al. 1990. Diabetes 39:815. (Activ)
  3. Havran WL, et al. 1988. J. Immunol. 140:1034 (Activ)
  4. Flanagan K, et al. 2008. J. Immunol. 180:3874. (IHC)
  5. Makaroff LE, et al. 2009. P. Natl. Acad. Sci. USA 106:4799. (FC)
  6. Zuber J, et al. 2009. Genes Dev. 23:877. (FC) PubMed
  7. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  8. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  9. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
Product Citations
  1. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
  2. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  3. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  4. Dyer DP et al. 2019. Immunity. 50(2):378-389 . PubMed
  5. Groza D, et al. 2018. Oncoimmunology. 7:e1424676. PubMed
  6. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  7. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  8. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  9. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  10. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  11. Lindborg JA et al. 2017. The Journal of Neuroscience. 37(43):10258-10277 . PubMed
  12. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  13. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  14. Deng M, et al. 2020. Nat Commun. 11:2193. PubMed
  15. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  16. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  17. Jin J, et al. 2020. Cell Reports. 30(12):4124-4136. PubMed
  18. Nacer A, et al. 2014. PLoS Pathog. 10:1004528. PubMed
  19. Velázquez F, et al. 2016. J Immunol. 196: 1305 - 1316. PubMed
  20. Kolawole A, et al. 2015. J Virol. 90: 1499-1506. PubMed
  21. Zhang X, et al. 2020. Elife. 9:00. PubMed
  22. Ritzel RM, et al. 2021. Glia. 69:746. PubMed
  23. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  24. Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed
  25. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  26. Hu Y, et al. 2021. Cell Death Dis. 12:743. PubMed
  27. Issah Y, et al. 2021. Elife. 10:. PubMed
  28. Li Y, et al. 2021. Brain Behav Immun. 91:267. PubMed
  29. Lee JH, et al. 2022. EBioMedicine. 77:103903. PubMed
  30. Suresh R, et al. 2020. J Immunother Cancer. 8:. PubMed
  31. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  32. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  33. Parrish KE, et al. 2021. Biopharm Drug Dispos. 42:137. PubMed
  34. Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  35. Denk D, et al. 2022. Immunity. 55:2059. PubMed
  36. Miró L, et al. 2022. Cancers (Basel). 14: . PubMed
  37. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  38. Drummer C, et al. 2023. Front Immunol. 14:1113883. PubMed
  39. Khan NZ, et al. 2021. Brain Behav Immun. 92:165. PubMed
  40. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  41. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  42. Le DT, et al. 2023. iScience. 26:106059. PubMed
  43. Ivan DC, et al. 2023. Acta Neuropathol Commun. 11:35. PubMed
  44. Xu H, et al. 2023. Elife. 12:. PubMed
  45. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  46. Wang X, et al. 2023. Stem Cell Rev Rep. . PubMed
  47. Carone M, et al. 2023. Nat Commun. 14:3489. PubMed
RRID
AB_10640119 (BioLegend Cat. No. 128023)
AB_10643270 (BioLegend Cat. No. 128024)

Antigen Details

Structure
14-17 kD protein (134 amino acids), member of the Ly-6 family of GPI linked protein. Ly6 family members share structure homology throughout a distinctive cystein rich protein domain that incorporates O-linked carbohydrates.
Distribution

Ly-6C is expressed primarily on bone marrow myeloid populations, monocytes/macrophages, neutrophils, endothelial cells, and some T cell subsets. Ly-6C is also a marker of memory CD8+ T cells.

Cell Type
Endothelial cells, Macrophages, Monocytes, Neutrophils, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
2. Cerwenka A, et al. 1998. J. Immunol. 161:97.

Gene ID
17067 View all products for this Gene ID
UniProt
View information about Ly-6C on UniProt.org
Go To Top Version: 3    Revision Date: 06/27/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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