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APC anti-human CD206 (MMR) Antibody

Pricing & Availability
Clone
15-2 (See other available formats)
Regulatory Status
RUO
Other Names
MMR (macrophage mannose receptor), MR (mannose receptor), CD206, MRC1
Isotype
Mouse IgG1, κ
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Product Citations
publications
15-2_APC_090707
GM-CSF- stimulated (day-3) human monocytes stained with 15-2 APC
  • 15-2_APC_090707
    GM-CSF- stimulated (day-3) human monocytes stained with 15-2 APC
See APC spectral data
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321109 25 tests $149
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321110 100 tests $314
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Description

Macrophage mannose receptor (MMR) is a 162-175 kD type I membrane protein also known as CD206, MRC1, or mannose receptor (MR). It is a pattern recognition receptor (PRR) that belongs to C-type lectin superfamily. MMR is expressed on macrophages, dendritic cells, and hepatic or lymphatic endothelial cells, but not on monocytes. MMR recognizes a range of microbial carbohydrates bearing mannose, fucose, or N-acetyl glucosamine. MMR mediates endocytosis and phagocytosis, induces activation of macrophages and antigen presentation, plays an important role in host defense, and provides a link between innate and adaptive immunity.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human mannose receptor
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 15-2 antibody blocks the interaction of MMR with its ligand, and inhibits mannose receptor-mediated degradation of t-PA by macrophages. Additional reported applications of this antibody (for the relevant formats) include: Western blotting1, blocking of ligand binding1,2, immunofluorescence3, and immunohistochemical staining of acetone-fixed frozen tissue sections1. The Utra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 321149 and 321150).

Application References

(PubMed link indicates BioLegend citation)
  1. Noorman F, et al. 1997. J. Leukocyte Biol. 61:63. (WB, IHC, Block)
  2. Barrett-Bergshoeff M, et al. 1997. Thromb Haemost. 77:718. (Block)
  3. Kato M, et al. 2007. J. Immunol. 179:6052. (IF)
Product Citations
  1. Shinohara H, et al. 2017. J Immunol. 10.4049/jimmunol.1700167. PubMed
  2. Unger A et al. 2018. Frontiers in immunology. 1.364583333 . PubMed
  3. Wang F, et al. 2018. Oncogenesis. 7:41. PubMed
  4. Bravo-Flores E, et al. 2018. Int J Mol Sci. 19:E1074. PubMed
  5. Blaszczak AM, et al. 2019. J Diabetes Res. 2019:8124563. PubMed
  6. Sohrabi Y, et al. 2019. Front Immunol. 9:3155. PubMed
  7. Ray SC, et al. 2018. J Immunol. 200:3568. PubMed
  8. de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed
  9. Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
  10. Li B, et al. 2019. Oncogenesis. 8:17. PubMed
  11. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  12. Sohrabi Y, et al. 2020. Front Immunol. 11:353. PubMed
  13. Yang L, et al. 2020. Cell Stem Cell. 27(1):125-136. PubMed
  14. Zhang B, et al. 2020. Cell Death Dis. 0.973611111. PubMed
  15. Yoshimura T, et al. 2013. PLoS One. 8:e58791. PubMed
  16. Edin S, et al. 2013. PLoS One. 18:74982. PubMed
  17. Nguyen A, et al. 2014. J Biol Chem. 289:1688. PubMed
  18. O'Regan N, et al. 2014. PLoS Negl Trop Dis. 8:3206. PubMed
  19. Deng W, et al. 2015. Clin Immunol. . PubMed
  20. Lundholm M, et al. 2015. Sci Rep. 5: 15651. PubMed
  21. Li Z, et al. 2020. J Immunol. 2589:204. PubMed
  22. Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed
  23. Que Y, et al. 2021. Front Immunol. 12:653030. PubMed
  24. Terenzi DC, et al. 2021. STAR Protoc. 2:100311. PubMed
  25. Song D, et al. 2021. Commun Biol. 4:500. PubMed
  26. Cui J, et al. 2021. J Cell Mol Med. 25:5586. PubMed
  27. Wang X, et al. 2021. Front Physiol. 12:644903. PubMed
  28. Stempin CC, et al. 2021. Cancers (Basel). 13:. PubMed
  29. Bekere I, et al. 2021. PLoS Pathog. 17:e1010074. PubMed
  30. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  31. Vijayakumar B, et al. 2022. Immunity. . PubMed
  32. Murugesan G, et al. 2022. Biomedicines. 10:. PubMed
  33. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  34. Hou ZH, et al. 2020. Am J Physiol Cell Physiol. 318:C649. PubMed
  35. Liu Y, et al. 2022. Clin Transl Med. 12:e654. PubMed
  36. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  37. Wu T, et al. 2022. Cell Death Dis. 13:624. PubMed
  38. Arlt A, et al. 2020. Mol Oncol. 14:571. PubMed
  39. Yang HL, et al. 2020. Am J Reprod Immunol. 84:e13261. PubMed
  40. Cui L, et al. 2021. Am J Reprod Immunol. 86:e13436. PubMed
  41. Khan A, et al. 2022. Commun Biol. 5:480. PubMed
  42. Sefik E, et al. 2022. Nature. 606:585. PubMed
  43. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  44. He X, et al. 2023. Int J Biol Sci. 19:331. PubMed
  45. Wang X, et al. 2022. Oncogene. 41:4259. PubMed
  46. Jiang Y, et al. 2023. Sci Adv. 9:eadc8933. PubMed
  47. Xu J, et al. 2023. J Immunother Cancer. 11:. PubMed
  48. Ji T, et al. 2023. Oncogenesis. 12:31. PubMed
  49. Wu L, et al. 2023. Cell Res. . PubMed
RRID
AB_571884 (BioLegend Cat. No. 321109)
AB_571885 (BioLegend Cat. No. 321110)

Antigen Details

Structure
Type I membrane protein, Pattern Recognition Receptor (PRR) family, C-type lectin superfamily, 162-175 kD
Distribution

Macrophages, dendritic cells, hepatic and lymphatic endothelial cells

Function
Pathogen binding, facilitate phagocytosis and endocytosis, macrophage activation and antigen presentation
Ligand/Receptor
Mannose, fucose, N-acetyl glucosamine
Cell Type
Dendritic cells, Endothelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Mason D, et al. Eds. 2002. Leukocyte Typing VII. Oxford University Press. p303
2. Wileman TE, et al. 1986. P. Natl. Acad. Sci. USA 83:2501.
3. Apostolopoulos V and McKenzie IF. 2001. Curr. Mol. Med. 1:469.
4. Le Cabec V, et al. 2005. J. Leukocyte Biol. 77:934.
5. Barrett-Bergshoeff M, et al. 1997. Thromb. Haemostatis 77:718.

Gene ID
4360 View all products for this Gene ID
UniProt
View information about CD206 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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