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APC anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
OKT4_APC_060507
Human peripheral blood lymphocytes stained with OKT4 APC
  • OKT4_APC_060507
    Human peripheral blood lymphocytes stained with OKT4 APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
317415 25 tests $33
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317416 100 tests $72
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.9

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
  9. Lederman S, et al. 1991. Mol Immunol. 28:1171-81.
Product Citations
  1. Dushyanthen S, et al. 2017. Nat Commun. 10.1038/s41467-017-00728-9. PubMed
  2. Azizi E et al. 2018. Cell. 174(5):1293-1308 e36. PubMed
  3. Want MY, et al. 2019. Oncoimmunology. 8:e1586042. PubMed
  4. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  5. Sun J, et al. 2018. Scand J Immunol. 88:e12674. PubMed
  6. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  7. Mujib S, et al. 2017. JCI Insight. 2:e93687. PubMed
  8. Arif S, et al. 2020. Diabetologia. 63(6):1186-1198.. PubMed
  9. Claiborne DT, et al. 2019. PLoS Pathog. 15:e1007981. PubMed
  10. Saadati S, et al. 2020. Avicenna J Med Biotechnol. 0.591666667. PubMed
  11. Kim K, et al. 2019. Nat Microbiol. 1.586111111. PubMed
  12. Wang A, et al. 2020. Front Immunol. 1.213194444. PubMed
  13. Kang HM, et al. 2018. Nat Biotechnol. 36:89. PubMed
  14. Altman K 2006. J Immunol . 177:1721. PubMed
  15. Sashihara J, et al. 2011. PLoS One. 6:e1002308. PubMed
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  17. Jafari M, et al. 2014. J Virol. 88:5062. PubMed
  18. Uhlenbrock F, et al. 2014. J Immunol. 193:1654. PubMed
  19. Frencher J, et al. 2014. J Leukoc Biol. 96:957. PubMed
  20. Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed
  21. Acerbi E, et al. 2016. Sci Rep. 6:23128. PubMed
  22. Comte D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9321 - 9326. PubMed
  23. Narsale A, Moya R, Robertson H 2016. Data Brief. 8: 1348-51. PubMed
  24. Sugita S, et al. 2016. Stem Cell Reports. 7:619-634. PubMed
  25. Johnson A, et al. 2016. mSphere. 1: e00288-16. PubMed
  26. Siedlik J, et al. 2017. J Immunol Methods. 10.1016/j.jim.2017.03.017. PubMed
  27. Li N, et al. 2020. Oncoimmunology. 9:1824643. PubMed
  28. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  29. Clayton KL, et al. 2021. Cell Host Microbe. 29(3):435-447.e9. PubMed
  30. Dupont L, et al. 2021. Cell Host Microbe. 29(5):792-805.e6. PubMed
  31. Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
  32. Su Y, et al. 2020. Cell. 1479:183. PubMed
  33. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  34. Berezhnoy A, et al. 2020. Cell Rep Med. 1:100163. PubMed
  35. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  36. Jiang H, et al. 2021. Oncoimmunology. 10:1943180. PubMed
  37. Carre C, et al. 2021. iScience. 24:102970. PubMed
  38. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  39. Wang Z, et al. 2021. Cell Mol Immunol. 18:2188. PubMed
  40. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  41. Zhu ZY, et al. 2022. Front Immunol. 12:781087. PubMed
  42. Mete B, et al. 2022. Life Sci Alliance. 5:. PubMed
  43. Reyes M, et al. 2021. Sci Transl Med. 13:. PubMed
  44. Bossini-Castillo L, et al. 2022. Cell Genom. 2:. PubMed
  45. Okutani Y, et al. 2022. Tissue Eng Part A. 28:94. PubMed
  46. Li N, et al. 2021. Cell Rep Med. 2:100297. PubMed
  47. Li N, et al. 2021. STAR Protoc. 2:100942. PubMed
  48. Wang T, et al. 2022. Front Immunol. 13:954121. PubMed
  49. Dahal S, et al. 2022. Retrovirology. 19:18. PubMed
  50. Zhang J, et al. 2022. Nature. 609:369. PubMed
  51. Ledderose C, et al. 2021. J Leukoc Biol. 109:497. PubMed
  52. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  53. Mitchell JL, et al. 2022. J Clin Invest. 132: . PubMed
  54. Höfle J, et al. 2022. EMBO Rep. 23:e54133. PubMed
  55. Wang CQ, et al. 2023. Cells. 12: . PubMed
  56. Zhang W, et al. 2022. Dev Cell. 57:329. PubMed
  57. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
RRID
AB_571944 (BioLegend Cat. No. 317415)
AB_571945 (BioLegend Cat. No. 317416)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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