- Clone
- 11A8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DNAM-1, PTA1, TLiSA1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![11A8_APCFire750_CD226_Antibody_030218_updated 11A8_APCFire750_CD226_Antibody_030218_updated](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/11A8_APCFire750_CD226_Antibody_030218_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes were stained with CD3 Brilliant Violet 421™ and CD226 (clone 11A8) APC/Fire™ 750 (left) or mouse IgG1, κ APC/Fire™ 750 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
338319 | 25 tests | $176 | ||||
338320 | 100 tests | $382 |
DNAM-1 (CD226) is a ~65 kD glycoprotein expressed on cell surface of T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells and a member of the immunoglobulin (Ig)-superfamily containing 2 Ig-like domains of the V-set. The ligands for CD226 are the poliovirus receptor (CD155) and its family member nectin-2 (CD112), which are broadly expressed on epithelial, endothelial and neuronal cells. CD226 is physically associated with LFA-1 in NK cells and activated T cells, and involved in LFA-1-mediated signaling.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications include: block NK cell-mediated lysis.
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Application References
(PubMed link indicates BioLegend citation) -
- Fuchs A, et al. 2004. J. Immunol. 172:3994.
- RRID
-
AB_2716145 (BioLegend Cat. No. 338319)
AB_2716146 (BioLegend Cat. No. 338320)
Antigen Details
- Structure
- Ig domain containing adhesion molecule
- Distribution
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Peripheral blood T cells, NK cells, monocytes/macrophages, platelets and megakaryocytes and a subset of B cells
- Function
- Receptor involved in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-lymphocyte (CTL) and NK cell
- Interaction
- Interacts with LFA-1.
- Ligand/Receptor
- PVR (CD155) and Nectin-2 (CD112)
- Cell Type
- B cells, Macrophages, Megakaryocytes, Monocytes, NK cells, Platelets, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
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1. Shibuya A et al. 1996. Immunity 4:573.
2. Tahara-Hanaoka S et al. 2004. Int. Immunol. 16:533.
3. Pende D et al. 2005. Mol. Immunol. 42:463. - Gene ID
- 10666 View all products for this Gene ID
- UniProt
- View information about CD226 on UniProt.org
Related Pages & Pathways
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