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Brilliant Violet 510™ anti-human CD16 Antibody

Pricing & Availability
Clone
3G8 (See other available formats)
Regulatory Status
RUO
Workshop
V NK80
Other Names
FcγRIII, Fc gamma receptor, Fc gamma receptor 3
Isotype
Mouse IgG1, κ
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Product Citations
publications
3G8_BV510_CD16_Antibody_FC_082312
Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 510™.
  • 3G8_BV510_CD16_Antibody_FC_082312
    Human peripheral blood lymphocytes were stained with CD16 (clone 3G8) Brilliant Violet 510™.
See Brilliant Violet 510™ spectral data
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302047 25 tests $180
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302048 100 tests $376
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Description

CD16 is known as low affinity IgG receptor III (FcγRIII). It is expressed as two distinct forms (CD16a and CD16b). CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. It is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. CD16b (FcγRIIIB) is a 48 kD glycosylphosphatidylinositol (GPI)-anchored protein. Its extracellular domain is over 95% homologous to that of CD16a, and it is expressed specifically on neutrophils. CD16 binds aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell-mediated cytotoxicity (ADCC).

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PMN cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

The 3G8 antibody clone blocks neutrophil phagocytosis and stimulates NK cell proliferation. It has been reported that this clone interacts with the FcγRIIa and FcγRIIIb receptors causing neutrophil activation and aggregation18. Due to this phenomenon staining in whole blood may cause a reduction in the number of granulocytes or alter their scatter profile.

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6, immunoprecipitation3, stimulation of NK cell proliferation4, blocking of phagocytosis5, and blocking of immunoglobulin binding to FcγRIII7,8. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302049, 302050, 302057, 302058).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Edberg J, et al. 1997. J. Immunol. 159:3849. (IP)
  4. Hoshino S, et al. 1991. Blood 78:3232. (Stim)
  5. Tamm A, et al. 1996. Immunol. 157:1576. (Block)
  6. Da Silva DM, et al. 2001. Int. Immunol. 13:633. (IHC)
  7. Holl V, et al. 2004. J. Immunol. 173:6274. (Block)
  8. Hober D, et al. 2002. J. Gen. Virol. 83:2169. (Block)
  9. Brainard DM, et al. 2009. J. Virol. 83:7305. PubMed
  10. Smed-Sörensen A, et al. 2008. Blood 111:5037. (Block) PubMed
  11. Timmerman KL, et al. 2008. J. Leukoc. Biol. 84:1271. (FC) PubMed
  12. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  13. Rout N, et al. 2010. PLoS One 5:e9787. (FC)
  14. Kim WK, et al. 2006. Am. J. Pathol. 168:822. (FC)
  15. Boltz A, et al. 2011. J. Biol Chem. 286:21896. PubMed
  16. Wu Z, et al. 2013. J. Virol. 87:7717. PubMed
  17. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  18. Vossebeld PJ, et al. 1997. Biochem J. 323:87-94 (Stim)
Product Citations
  1. Bolton D, et al. 2017. PLoS Pathogens. 13(6):e1006445. PubMed
  2. Liu Y, et al. 2019. Nat Commun. 10:324. PubMed
  3. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
  4. Dos Santos JC, et al. 2019. Cell Rep. 28:2659. PubMed
  5. Rohner L, et al. 2020. Sci Rep. 0.570833333. PubMed
  6. Wang H, et al. 2020. Onco Targets Ther. 13:3903. PubMed
  7. Cirovic B, et al. 2020. Cell Host & Microbe. 28(2):322-334. PubMed
  8. Colby DJ, et al. 2018. Nat Med. 24:923. PubMed
  9. Martins M, et al. 2015. J Virol. 89: 10802 - 10820. PubMed
  10. Wheatley A, et al. 2016. Sci Rep. 6: 26478. PubMed
  11. Wahl S, et al. 2016. Nature. 541:81-86. PubMed
  12. Juno JA, et al. 2020. Nat Med. 26:1428. PubMed
  13. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  14. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  15. Zabaleta N, et al. 2021. Cell Host Microbe. :. PubMed
  16. Nguyen THO, et al. 2021. Immunity. 54:1066. PubMed
  17. Pi J, et al. 2022. J Nanobiotechnology. 20:36. PubMed
  18. Maheshwari D, et al. 2022. iScience. 25:104384. PubMed
  19. Wheatley AK, et al. 2021. Cell Rep. 37:109822. PubMed
  20. Liisborg C, et al. 2022. PLoS One. 17:e0269960. PubMed
  21. Herati RS, et al. 2022. Nat Immunol. 23:1183. PubMed
  22. Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
  23. Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
  24. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  25. Nasiri B, et al. 2022. Adv Healthc Mater. 11:e2200890. PubMed
  26. Jacobs ME, et al. 2021. Immunother Adv. 1:ltaa004. PubMed
  27. Kraft JD, et al. 2022. FASEB J. 36:e22173. PubMed
  28. Koutsakos M, et al. 2022. Immunity. 55:1316. PubMed
  29. Chen J, et al. 2023. Antib Ther. 6:38. PubMed
  30. Pioch J, et al. 2022. J Immunol Methods. 507:113308. PubMed
  31. Bohrer AC, et al. 2022. Cell Rep. 40:111144. PubMed
  32. Liisborg C, et al. 2022. Acta Ophthalmol. :3. PubMed
  33. Abarca-Zabalía J, et al. 2023. Front Immunol. 14:1127352. PubMed
  34. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
RRID
AB_2561380 (BioLegend Cat. No. 302047)
AB_2562085 (BioLegend Cat. No. 302048)

Antigen Details

Structure
Ig superfamily, transmembrane form (50-65 kD) or GPI-linked form (48 kD)
Distribution

NK cells, activated monocytes, macrophages, neutrophils

Function
Low affinity IgG Fc receptor, phagocytosis, ADCC
Ligand/Receptor
Aggregated IgG, IgG-antigen complex
Cell Type
Dendritic cells, Macrophages, Monocytes, Neutrophils, NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References

1. Fleit H, et al. 1982. P. Natl. Acad. Sci. USA 79:3275.
2. Stroncek D, et al. 1991. Blood 77:1572.
3. Wirthmueller U, et al. 1992. J. Exp. Med. 175:1381.

Gene ID
2214 View all products for this Gene ID
UniProt
View information about CD16 on UniProt.org

Related FAQs

Is our human Trustain FcX™ (cat# 422302) compatible with anti human CD16, CD32 and CD64 clones 3G8, FUN-2 and 10.1 respectively?

Yes

Go To Top Version: 2    Revision Date: 05/23/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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