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Brilliant Violet 785™ anti-mouse CD8a Antibody

Pricing & Availability
Clone
53-6.7 (See other available formats)
Regulatory Status
RUO
Other Names
T8, Lyt2, Ly-2
Isotype
Rat IgG2a, κ
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Product Citations
publications
53-6.7_BV785_061812
C57BL/6 mouse splenocytes were stained with CD3 PE and CD8a (clone 53-6.7) Brilliant Violet 785™.
  • 53-6.7_BV785_061812
    C57BL/6 mouse splenocytes were stained with CD3 PE and CD8a (clone 53-6.7) Brilliant Violet 785™.
See Brilliant Violet 785™ spectral data
Cat # Size Price Quantity Check Availability Save
100749 125 µL $209
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100750 50 µg $273
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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunofluorescent staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes3. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation1,3, in vivo and in vitro cell depletion2,10,15, inhibition of CD8 T cell proliferation3, blocking of cytotoxicity3,4, immunohistochemical staining5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IHC, IP)
  2. Hathcock KS. 1991. Current Protocols in Immunology. 3.4.1. (Deplete)
  3. Takahashi K, et al. 1992. P. Natl. Acad. Sci. USA 89:5557. (Block, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:1503. (Block)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Kamimura D, et al. 2006. J. Immunol. 177:306.
  9. Bouwer HGA, et al. 2006. P. Natl. Acad. Sci. USA 103:5102. (FC, Deplete)
  10. Kao C, et al. 2005. Int. Immunol. 17:1607. PubMed
  11. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  12. Rasmussen JW, et al. 2006. Infect. Immun. 74:6590. PubMed
  13. Lee CH, et al. 2009. Clin. Cancer Res. PubMed
  14. Geiben-Lynn R, et al. 2008. Blood 112:4585. (Deplete) PubMed
  15. Kingeter LM, et al. 2008. J. Immunol. 181:6244. PubMed
  16. Guo Y, et al. 2008. Blood 112:480. PubMed
  17. Andrews DM, et al. 2008. J. Virol. 82:4931. PubMed
  18. Britschqui MR, et al. 2008. J. Immunol. 181:7681. PubMed
  19. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  20. Jordan JM, et al. 2008. Infect. Immun. 76:3717. PubMed
  21. Todd DJ, et al. 2009. J. Exp. Med. 206:2151. PubMed
  22. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  23. Medyouf H, et al. 2010. Blood 115:1175. PubMed
  24. Riedl P, et al. 2009. J. Immunol. 183:370. PubMed
  25. Apte SH, et al. 2010. J. Immunol. 185:998. PubMed
  26. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  27. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  28. Cui L, et al. 2015. J Control Release. 206:220. PubMed
  29. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  30. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Limbach K, et al. 2017. Malar J. 10.1186/s12936-017-1911-z. PubMed
  2. Tran C, et al. 2017. J Immunol. 10.4049/jimmunol.1600396. PubMed
  3. Halim TYF et al. 2018. Immunity. 48(6):1195-1207 . PubMed
  4. Staffas A et al. 2018. Cell host & microbe. 23(4):447-457 . PubMed
  5. Beura LK, et al. 2018. Immunity. 48:327. PubMed
  6. Brown SM et al. 2017. Endocrinology. 158(10):3592-3604 . PubMed
  7. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  8. Lee DH, et al. 2019. PLoS One. 14:e0215727. PubMed
  9. Albaghdadi AJH, et al. 2019. Sci Rep. 9:6528. PubMed
  10. Casulli J, et al. 2019. Nat Commun. 10:2121. PubMed
  11. Deasy SK, et al. 2019. PLoS Genet. 15:e1008020. PubMed
  12. Poillet–Perez L, et al. 2018. Nature. 563:569. PubMed
  13. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  14. Komuczki J, et al. 2019. Immunity. 50:1289. PubMed
  15. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  16. Gagnon JD, et al. 2019. Cell Rep. 28:2169. PubMed
  17. Gao J et al. 2016. Cell. 167(2):397-404 . PubMed
  18. Parks CA, et al. 2019. Proc Natl Acad Sci U S A. 116:3136. PubMed
  19. Walsh CM, et al. 2019. Elife. 8:e48448. PubMed
  20. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  21. Li J, et al. 2018. Immunity. 49:178. PubMed
  22. Lee H, et al. 2020. Cell Metabolism. 31(4):822-836. PubMed
  23. Krummey SM, et al. 2020. Cell Reports. 30(5):1282-1291.e5.. PubMed
  24. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  25. Morris AB, et al. 2020. Immunity. 52(1):136-150.e6.. PubMed
  26. Chen H, et al. 2005. J Immunol. 175:591. PubMed
  27. Ballet R, et al. 2014. PLoS Pathog. 10:1004550. PubMed
  28. Gordon E, et al. 2015. Proc Natl Acad Sci U S A. 112: 13075 - 13080. PubMed
  29. Cowan J, et al. 2016. Cell Rep. 14:1041-1048. PubMed
  30. Wei H, et al. 2016. J Immunol. 196: 3537 - 3541. PubMed
  31. Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed
  32. Vardhana SA, et al. 2020. Nat Immunol. 1.584722222. PubMed
  33. Kim EH, et al. 2020. Elife. 9:00. PubMed
  34. Glassman CR, et al. 2021. eLife. 10:00. PubMed
  35. Milner JJ, et al. 2020. Immunity. 52(5):808-824.e7. PubMed
  36. Kumagai S, et al. 2020. Immunity. 53(1):187-203.e8. PubMed
  37. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  38. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  39. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  40. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
  41. Glassman CR, et al. 2021. Cell. 184(4):983-999.e24. PubMed
  42. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  43. Mitchell JE, et al. 2021. Cell Reports. 35(2):108966. PubMed
  44. Devi S, et al. 2021. Immunity. 54(6):1219-1230.e7. PubMed
  45. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  46. Marangoni F, et al. 2021. Cell. . PubMed
  47. Ayasoufi K, et al. 2020. Brain. 3629:143. PubMed
  48. Molgora M, et al. 2020. Cell. 182:886. PubMed
  49. Canton J, et al. 2021. Nat Immunol. 22:140. PubMed
  50. Poillet-Perez L, et al. 2020. Nat Cancer. 1:923. PubMed
  51. Feizi N, et al. 2021. Cell Death Dis. 12:1026. PubMed
  52. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  53. Kuhn JA, et al. 2021. Elife. 10:. PubMed
  54. Zhou R, et al. 2022. EBioMedicine. 75:103762. PubMed
  55. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  56. Synn CB, et al. 2022. Clin Transl Immunology. 11:e1364. PubMed
  57. Zhu G, et al. 2022. Front Microbiol. 12:806902. PubMed
  58. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  59. Luo J, et al. 2022. J Nanobiotechnology. 20:228. PubMed
  60. Lefebvre MN, et al. 2021. Cell Rep. 37:109956. PubMed
  61. Fang F, et al. 2022. JCI Insight. 7:. PubMed
  62. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  63. Tuong ZK, et al. 2021. Cell Rep. 37:110132. PubMed
  64. Tomala J, et al. 2020. Methods Mol Biol. 2111:101. PubMed
  65. Zhang W, et al. 2022. Nat Methods. 19:759. PubMed
  66. Quijano-Rubio A, et al. 2022. Nat Biotechnol. Online ahead of print. PubMed
  67. Martens PJ, et al. 2022. Front Endocrinol (Lausanne). 13:1023264. PubMed
  68. Abou-Hamad J, et al. 2022. iScience. 25:105524. PubMed
  69. Mancini M, et al. 2021. Sci Rep. 11:21171. PubMed
  70. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  71. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  72. van Dierendonck XAMH, et al. 2022. Proc Natl Acad Sci U S A. 119:e2114739119. PubMed
  73. Reticker-Flynn NE, et al. 2022. Cell. 185:1924. PubMed
  74. Belk JA, et al. 2022. Cancer Cell. 40:768. PubMed
  75. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  76. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  77. Zhao Q, et al. 2023. Signal Transduct Target Ther. 8:40. PubMed
  78. Houlder E, et al. 2023. Nat Commun. 14:1863. PubMed
  79. Ito M, et al. 2023. Sci Rep. 13:5939. PubMed
RRID
AB_11218801 (BioLegend Cat. No. 100749)
AB_2562610 (BioLegend Cat. No. 100750)

Antigen Details

Structure
Ig superfamily, CD8α chain, 34 kD
Distribution

Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.

Gene ID
12525 View all products for this Gene ID
UniProt
View information about CD8alpha on UniProt.org
Go To Top Version: 2    Revision Date: 01/21/2014

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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