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FITC anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Regulatory Status
RUO
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
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Product Citations
publications
BC96_FITC_062107
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • BC96_FITC_062107
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) FITC (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
See FITC spectral data
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302603 25 tests $54
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302604 100 tests $118
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Meniailo M, et al. 2017. Int Immunopharmacol. 10.1016/j.intimp.2017.06.023. PubMed
  2. Pauthner MG, et al. 2019. Immunity. 50:241. PubMed
  3. Cirelli KM et al. 2019. Cell. 177(5):1153-1171 . PubMed
  4. Garcia–Perez JE, et al. 2019. Front Immunol. 10:998. PubMed
  5. Kubo M, et al. 2018. Oncol Rep. 39:417. PubMed
  6. Qu K et al. 2017. Cancer cell. 32(1):27-41 . PubMed
  7. Yost KE, et al. 2019. Nat Med. 25:1251. PubMed
  8. Wang H, et al. 2020. Mol Oncol. 14:991. PubMed
  9. Beswick E, et al. 2011. Infect Immun. 79:2737. PubMed
  10. Businaro R, et al. 2009. Atherosclerosis. 207:74. PubMed
  11. Ryba M, et al. 2011. Cytokine. 55:353. PubMed
  12. Miles B, et al. 2015. Nat Commun. 6: 8608. PubMed
  13. Rhijn I, et al. 2016. Proc Natl Acad Sci U S A. 113: 380 - 385. PubMed
  14. Jiang J, et al. 2016. Sci Rep. 6: 32320. PubMed
  15. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  16. Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed
  17. Rousso-Noori L, et al. 2021. Nat Commun. 12:3615. PubMed
  18. Kim ST, et al. 2021. J Immunother Cancer. 9:. PubMed
  19. Liu H, et al. 2021. J Immunother Cancer. 9:. PubMed
  20. Hassani M, et al. 2020. Iran Biomed J. 24:81. PubMed
  21. Turner AW, et al. 2020. ACS Infect Dis. 6:1719. PubMed
  22. Grigoriou M, et al. 2021. Cancer Immunol Res. 9:726. PubMed
  23. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  24. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  25. Grivas A, et al. 2022. Front Immunol. 13:964274. PubMed
  26. Thiramanas R, et al. 2020. Cells. 9: . PubMed
  27. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  28. Gartshteyn Y, et al. 2023. Life Sci Alliance. 6: . PubMed
  29. Felce SL, et al. 2021. Wellcome Open Res. 5:42. PubMed
  30. Metelo AM, et al. 2022. Cancer Res Commun. 2:158. PubMed
  31. Donadei C, et al. 2023. J Clin Med. 12:. PubMed
RRID
AB_314273 (BioLegend Cat. No. 302603)
AB_314274 (BioLegend Cat. No. 302604)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 3    Revision Date: 12/15/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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