ELISA MAX™ Deluxe Set Human IL-6

Kit Contents

Kit Contents

• Human IL-6 ELISA MAX™ Capture Antibody (200X)
• Human IL-6 ELISA MAX™ Detection Antibody (200X)
• Human IL-6 Standard
• Avidin-HRP (1000X)
• Substrate Solution A
• Substrate Solution B
• Coating Buffer A (5X)
• Assay Diluent A (5X)

Product Details

Reactivity

Human

Application

ELISA

 

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Application References

(PubMed link indicates BioLegend citation)

1. Ishola Jr DA, et al. 2006. Kidney International 2006; doi:10.1038/sj.ki.5001629
2. Kim I, et al. 2006. Blood doi:10.1182/blood-2006-04-014399
3. Wimmer A, et al. 2006. Blood 108:3722.
4. Kim SR, et al. 2009. Biochem Biophys Res Commun. 387:342. PubMed
5. Samuvel DJ, et al. 2010. J. Biol Chem. 285:3916. PubMed
6. Tanavalee A, et al. 2011. J. Bone Joint Surg Br. 93:1065. PubMed
7. Parti PP, et al. 2011. Microbes Infect. 13:545. PubMed
8. 8. Abe K, et al. 2013. PLoS One. 8:e53620. PubMed
9. Cho HY, et al. 2014. Cell Immunol. 289:27. PubMed

Product Citations

1. Bang C, et al. 2017. PLoS One. 12(6):e0178541. PubMed
2. Thomas A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183542. PubMed
3. Choi J, et al. 2018. Nucleic Acids Res. 46:1912. PubMed
4. Dasari C, et al. 2017. Tumour Biol. 39:1010428317698380. PubMed
5. Ling C, et al. 2019. Protein Cell. 10:249. PubMed
6. Kolar SL, et al. 2019. JCI Insight. 4:5. PubMed
7. Sadewasser A, et al. 2019. Mol Ther Nucleic Acids. 16:686. PubMed
8. Chang YW, et al. 2018. Am J Physiol Lung Cell Mol Physiol. 314:L654. PubMed
9. Jasso–Miranda C, et al. 2019. Infect Drug Resist. 12:1833. PubMed
10. Samuvel D, et al. 2010. J Biol Chem. 285:3916. PubMed
11. Tanavalee A, et al. 2011. J Bone Joint Surg Br. 93:1065. PubMed
12. Abe K, et al. 2013. PLoS One. 8:e53620. PubMed
13. Parti R, et al. 2011. Microbes Infect. 13:545. PubMed
14. Kim S, et al. 2009. Biochem Biophys Res Commun. 387:342. PubMed
15. Cho H, Lee S 2014. Cell Immunol. 289:27. PubMed
16. Mendes B, et al. 2015. Int J Pharmaceutics. 490: 94-101. PubMed
17. Bader A, et al. 2015. PLoS One. 10: 0138477. PubMed
18. Kunyanee C, et al. 2016. PLoS One. 11: 0160741. PubMed
19. Millrine D, et al. 2016. Proc Natl Acad Sci U S A. 113: 10625 - 10630. PubMed
20. CarriÓn M, et al. 2016. J Leukoc Biol. 100(6):1385-1393. PubMed
21. Takeshita Y, et al. 2016. Neurol Neuroimmunol Neuroinflamm. 4(1):e311. PubMed
22. Zambrowicz A, et al. 2017. Food Chem. 10.1016/j.foodchem.2017.03.101. PubMed
23. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
24. Abdisalaam S, et al. 2020. J Biol Chem. 295:11144. PubMed
25. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
26. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
27. Santa Cruz A, et al. 2021. Front Immunol. 613422:12. PubMed
28. Chen Y, et al. 2021. Front Immunol. 12:623610. PubMed
29. Zhou J, et al. 2021. Front Immunol. 12:656242. PubMed
30. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
31. Li G, et al. 2021. Gut Microbes. 13:1968257. PubMed
32. Plesa M, et al. 2021. Front Immunol. 12:715848. PubMed
33. Galganska H, et al. 2021. Cell Mol Life Sci. 78:8229. PubMed
34. Kumar P, et al. 2021. Front Immunol. 12:694865. PubMed
35. Kamdee K, et al. 2021. Biomed Rep. 15:78. PubMed
36. Soto-Mercado V, et al. 2021. Biomolecules. 11:. PubMed
37. Martincuks A, et al. 2021. Front Oncol. 11:724104. PubMed
38. Zhang L, et al. 2022. Nat Commun. 13:360. PubMed
39. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
40. Gill PS, et al. 2022. Front Immunol. 13:872652. PubMed
41. Hamel Y, et al. 2021. Cell Rep Med. 2:100370. PubMed
42. Pan Y, et al. 2021. Br J Pharmacol. 178:4907. PubMed
43. Li Y, et al. 2021. J Neurochem. 159:867. PubMed
44. Pott F, et al. 2021. Emerg Microbes Infect. 10:2151. PubMed
45. Antoon R, et al. 2022. Cytotherapy. 24:699. PubMed
46. Mendes-Frias A, et al. 2022. Sci Rep. 12:17047. PubMed
47. Zhang W, et al. 2022. Front Pharmacol. 13:1031438. PubMed
48. Abdisalaam S, et al. 2022. Nucleic Acids Res. 50:2681. PubMed
49. Saxena V, et al. 2022. Cell Rep. 39:110727. PubMed
50. Chu HS, et al. 2022. Invest Ophthalmol Vis Sci. 63:1. PubMed
51. Gradzka-Boberda S, et al. 2022. J Virol. 96:e0121222. PubMed
52. Walker AR, et al. 2022. Cell Rep. 40:111318. PubMed
53. Jhuang KF, et al. 2023. Immunology. 169:344. PubMed

Sensitivity

4 pg/mL

Standard Range

7.8-500 pg/mL

Materials Not Included

• Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
• A microplate reader capable of measuring absorbance at 450 nm
• Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
• Deionized (DI) water
• PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na₂HPO₄, 0.2 g KH₂PO₄, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
• Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
• Wash bottle or automated microplate washer
• Stop Solution (e.g. 2N H₂SO₄) Biolegend Cat. No. 423001 is recommended
• Log-Log graph paper or software for data analysis
• Tubes to prepare standard dilutions
• Timer
• Plate Sealer
• Absorbent paper

Antigen Details

Cell Sources

T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells

Biology Area

Immunology, Innate Immunity

Molecular Family

Cytokines/Chemokines

Gene ID

3569 View all products for this Gene ID

UniProt

View information about IL-6 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis