- Clone
- 16B12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_16B12_PE_HA11_Antibody_072023 A_16B12_PE_HA11_Antibody_072023](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/16B12_PE_HA11_Antibody_072023.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Transfected RBL1 cells expressing the HA tag on the cell surface were stained with anti-HA.11 Epitope Tag (clone 16B12) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram). -
CHO-K1 cells (open histogram) or HA tag stably transfected cells (filled histogram) were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with HA.11 Epitope Tag (clone 16B12) PE.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
901517 | 25 µg | $189 | ||||
901518 | 100 µg | $469 |
The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.
Product DetailsProduct Details
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For intracellular flow cytometric staining using our True-Phos™ Perm Buffer in Cell Suspensions Protocol, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus. -
Application References
(PubMed link indicates BioLegend citation) -
- Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
- Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
- Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
- Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
- Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
- Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed
- Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed
- Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed
- Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
- Liu Z, et al. 2016. Nature. 530:98-102. PubMed
- Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed
- Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed
- Rodríguez-Escudero M, et al. 2016. PLoS One. 11:0148032. PubMed
- Lehmann W, et al. 2016. Nat Commun. 7:10498. PubMed
- Testoni E, et al. 2016. EMBO Mol Med. 8: 105-16. PubMed
- Padilla S, et al. 2016. Nat Neurosci. 10.1038/nn.4274. PubMed
- Martins J, et al. 2016. J Cell Sci. 129:1271-82. PubMed
- Matak P, et al. 2016. Proc Natl Acad Sci U S A. 113:3428-35. PubMed
- Starokadomskyy P, et al. 2016. Nat Immunol. 17:495-504. PubMed
- Mitxelena J, et al. 2016. Nucleic Acids Res. 44:5557-70. PubMed
- Thongthip S, et al. 2016. Genes Dev. 30:645-59. PubMed
- Aaes T, et al. 2016. Cell Rep. 15:274-87. PubMed
- Hodge C, et al. 2016. J Biol Chem. 291:9396-9410. PubMed
- Alagramam K, et al. 2016. Nat Chem Biol. 12:444-51. PubMed
- Veit G, et al. 2016. PLoS Biol. 14:1002462. PubMed
- Lee B, et al. 2016. Development. 143:1721-31. PubMed
- Douchi D, et al. 2016. Plant Cell. 28:1182-99. PubMed
- Avgousti D, et al. 2016. Nature. 535:173-77. PubMed
- Shin H, et al. 2015. Nature. 534:553-7. PubMed
- Gross G, et al. 2016. Nat Methods. 10.1038/nmeth.3894. PubMed
- Dick M, et al. 2016. Nat Commun. 7:11929. PubMed
- Aldrin-Kirk P, et al. 2016. Neuron. 90:955-68. PubMed
- Fan R, et al. 2016. Nat Med. 22:780-91. PubMed
- Rowald K, et al. 2016. Cell Rep. 15:2679-91. PubMed
- Rampal R, Awasthi A, Ahuja V 2016. Development. 143:2334-43. PubMed
- Faden F, et al. 2016. Nat Commun. 7:12202. PubMed
- Lawson C, et al. 2016. Cancer Res. 76: 3826-37. PubMed
- Szargel R, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw189.. PubMed
- Damez-Werno D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9623-28. PubMed
- Su P, et al. 2016. J Immunol. 197: 1054-64. PubMed
- Morozumi Y, et al. 2016. J Mol Cell Biol. 8:349-62. PubMed
- Miura Y, et al. 2016. Biochem J. 473:2591-602. PubMed
- Pashkova N, et al. 2016. Cell Rep. 17:303-15. PubMed
- Sanchez M, et al. 2016. J Biol Chem. 291:19760-73. PubMed
- Hwang J, Lee J, Pallas D 2016. J Biol Chem. 291:21008-19. PubMed
- Jiménez-Canino R, et al. 2016. J Biol Chem. 291:19068-78. PubMed
- Barquilla A, et al. 2016. Mol Biol Cell. 27:2757-70. PubMed
- Yadav S, et al. 2016. Sci Rep. 6:34100. PubMed
- Hashimoto A, et al. 2016. Oncogenesis. 0.388194444. PubMed
- Guirouilh-Barbat J, et al. 2016. PLoS Genet. 12:e1006230. PubMed
- Gómez-H L, et al. 2016. Nat Commun. 7:13298. PubMed
- Rademacher N, et al. 2016. Sci Rep. 6:35283. PubMed
- Lin Z, et al. 2016. Nat Genet. 10.1038/ng.3701. PubMed
- Kaczmarska Z, et al. 2016. Nat Chem Biol. 10.1038/nchembio.2217. PubMed
- Zee B, et al. 2016. PLoS One. 11:e0163820. PubMed
- Despras E, et al. 2016. Nat Commun. 7:13326. PubMed
- Zhang H, et al. 2016. J Neurosci. 36:11837-50. PubMed
- Zhang M, et al. 2016. Cell Res. 26:1302-19. PubMed
- Baehr C, et al. 2016. J Biol Chem. 291:26875-85. PubMed
- Boehm E, et al. 2016. J Biol Chem. 291:25877-87. PubMed
- Gail Kilroy, et al. 2016. J Biol Chem. 291:27289-97. PubMed
- Hongchen Cai, Aimin Liu 2016. Proc Natl Acad Sci U S A. 113:14751-6. PubMed
- Tam K, et al. 2016. MBio. 7:e02024-16. PubMed
- Hanke L, et al. 2016. MBio. 7(6). pii: e01569-16. PubMed
- Stoeber M, et al. 2016. Proc Natl Acad Sci U S A. 113(50):E8069-E8078. PubMed
- Gu Q, et al. 2016. J Virol. 90:10545-57. PubMed
- Ramachandran S, et al. 2016. J Biol Chem. 291: 25489-504. PubMed
- Kaufmann T, et al. 2016. J Cell Sci. 129:4607-21. PubMed
- Piwko W, et al. 2016. EMBO J. 35:2584-601. PubMed
- Matsuo Y, et al. 2016. J Cell Sci. 129:4592-606. PubMed
- Athmer J, et al. 2017. MBio. 10.1128/mBio.02320-16. PubMed
- Gao L, et al. 2017. J Cell Sci. 130:396-405. PubMed
- Gong Y, et al. 2017. Genes Dev. 31:46-58. PubMed
- K Kataoka, K Mochizuki 2017. J Cell Sci. 130:480-9. PubMed
- Liszczak G, et al. 2017. Proc Natl Acad Sci U S A. 114:681-6. PubMed
- Haggie P, et al. 2017. J Biol Chem. 292:771-85. PubMed
- T Taetzsch, et al. 2017. J Neurosci. 37:70-82. PubMed
- Yagita Y, et al. 2017. J Biol Chem. 292:691-705. PubMed
- Chong Jiang, et al. 2017. J Biol Chem. 292:3137-45. PubMed
- Maeda R, et al. 2017. J Biol Chem. 292:3201-12. PubMed
- Davis RB, et al. 2017. JCI Insight. 2:e92465. PubMed
- Daniel JA, et al. 2017. Elife. 6:e26338. PubMed
- Oishi A, et al. 2017. Sci Rep. 7:8990. PubMed
- Product Citations
- RRID
-
AB_2629622 (BioLegend Cat. No. 901517)
AB_2629623 (BioLegend Cat. No. 901518)
Antigen Details
- Biology Area
- Cell Biology
- Gene ID
- NA
- UniProt
- View information about HA.11 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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