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PE anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
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Product Citations
publications
M5E2_PE_061507
Human peripheral blood monocytes stained with M5E2 PE
  • M5E2_PE_061507
    Human peripheral blood monocytes stained with M5E2 PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
301805 25 tests $65
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301806 100 tests $141
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301850 100 µg $146
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Kho D, et al. 2017. PLoS One. 10.1371/journal.pone.0180267. PubMed
  2. Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
  3. Crippa S, et al. 2019. J Clin Invest. 129:1566. PubMed
  4. Nemati S, et al. 2019. J Tissue Eng Regen Med. 13:25. PubMed
  5. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  6. Lin HT, et al. 2019. Int J Mol Med. 43:1085. PubMed
  7. Foote JR, et al. 2019. Front Immunol. 10:188. PubMed
  8. Zhang D, et al. 2020. Oncoimmunology. 9:1744921. PubMed
  9. Gomez I, et al. 2020. Nat Commun. 11:214. PubMed
  10. Kuwada K, et al. 2018. J Exp Clin Cancer Res. 37:307. PubMed
  11. Ravenhill BJ, et al. 2020. Sci Rep. 10:4560. PubMed
  12. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  13. Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
  14. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  15. Srisuwan S, et al. 2014. PLoS One. 9:110321. PubMed
  16. Fisher J, et al. 2014. Clin Cancer Res. 20:5720. PubMed
  17. Foster G, et al. 2015. J Immunol. 195: 5380 - 5392. PubMed
  18. Tatematsu M, et al. 2016. J Immunol. 196: 3865 - 3876. PubMed
  19. Eriksson E, et al. 2016. Gene Ther. 10.1038/gt.2016.80. PubMed
  20. Dhariwala MO, et al. 2020. Cell Rep Med. 1:100132. PubMed
  21. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  22. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  23. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  24. Wang X, et al. 2021. Immunity. 54(6):1123-1136.e8. PubMed
  25. Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
  26. Yang C, et al. 2021. J Invest Dermatol. 810:141. PubMed
  27. Bailey AL, et al. 2020. bioRxiv. . PubMed
  28. Weitz JR, et al. 2020. Diabetes. 69:1206. PubMed
  29. Bekere I, et al. 2021. PLoS Pathog. 17:e1010074. PubMed
  30. Miyamoto T, et al. 2021. Cancer Immunol Res. Online ahead of print. PubMed
  31. Wood MP, et al. 2020. J Infect Dis. 222:44. PubMed
  32. Tripathi H, et al. 2020. J Mol Cell Cardiol. 149:95. PubMed
  33. O'Neill MB, et al. 2021. Front Immunol. 12:768189. PubMed
  34. Li Y, et al. 2022. Cell Rep. 38:110284. PubMed
  35. Luo W, et al. 2022. Front Immunol. 13:816761. PubMed
  36. Gopinath A, et al. 2022. NPJ Parkinsons Dis. 8:72. PubMed
  37. Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed
  38. Babania O, et al. 2021. J Clin Lab Anal. 35:e23984. PubMed
  39. Kerdidani D, et al. 2022. J Exp Med. 219:. PubMed
  40. Karantanos T, et al. 2022. Sci Adv. 8:eabl8952. PubMed
  41. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  42. Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
  43. Costa MHG, et al. 2021. Biotechnol J. 16:e2000389. PubMed
  44. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  45. Polisetti N, et al. 2022. Bio Protoc. 12: . PubMed
  46. Hamdan F, et al. 2023. Mol Ther Oncolytics. 28:264. PubMed
  47. Wu XH, et al. 2023. Nat Commun. 14:1820. PubMed
  48. Okano F, et al. 2023. Cancer Res Commun. 3:640. PubMed
  49. Li J, et al. 2023. Sci Rep. 13:7501. PubMed
RRID
AB_314187 (BioLegend Cat. No. 301805)
AB_314188 (BioLegend Cat. No. 301806)
AB_2564138 (BioLegend Cat. No. 301850)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 11/05/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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