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PE anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HIT3a
Human peripheral blood lymphocytes stained with HIT3a PE
  • HIT3a
    Human peripheral blood lymphocytes stained with HIT3a PE
See PE spectral data
Cat # Size Price Quantity Check Availability Save
300307 25 tests $28
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300308 100 tests $61
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  2. Lin GL, et al. 2018. Acta Neuropathol Commun. 9:617. PubMed
  3. Rochat MA, et al. 2018. Virol J. 15:191. PubMed
  4. Blandine Monel et al. 2019. Cell reports. 27(1):142-153 . PubMed
  5. Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
  6. Usmani SM et al. 2019. Cell host & microbe. 25(1):73-86 . PubMed
  7. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  8. Mujib S, et al. 2017. JCI Insight. 2:e93687. PubMed
  9. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  10. Hu JQ, et al. 2020. J Cancer. 3.409722222. PubMed
  11. Łyszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed
  12. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  13. Wang H, et al. 2020. J Exp Clin Cancer Res. 39:29. PubMed
  14. Bardet M, et al. 2018. Immunol Cell Biol. 96:81. PubMed
  15. Liu F, et al. 2020. Oncoimmunology. 9:1746573. PubMed
  16. Zhai Y, et al. 2018. Autophagy. 1.714583333. PubMed
  17. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  18. Wu W, et al. 2020. Oncol Lett. 0.91875. PubMed
  19. Enghard P, et al. 2014. Ann Rheum Dis. 73:277. PubMed
  20. Galindo-Albarrán A, et al. 2016. Cell Rep. 17:2151-2160. PubMed
  21. Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
  22. Park JA, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  23. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  24. Mori A, et al. 2021. Cells. :10. PubMed
  25. Wu M, et al. 2021. Nat Commun. 12:3500. PubMed
  26. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  27. Liu J, et al. 2021. Medicine (Baltimore). 100:e24619. PubMed
  28. Chulpanova DS, et al. 2021. Biology (Basel). 10:. PubMed
  29. Platten M, et al. 2021. Nature. 592:463. PubMed
  30. Cheng J, et al. 2021. Biomed Res Int. 2021:6614784. PubMed
  31. Ma C, et al. 2021. Signal Transduct Target Ther. 6:353. PubMed
  32. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  33. Valestrand L, et al. 2022. Am J Pathol. . PubMed
  34. Pekayvaz K, et al. 2022. Nat Commun. 13:1018. PubMed
  35. Wu Y, et al. 2021. Cancer Gene Ther. Online ahead of print. PubMed
  36. Wang X, et al. 2022. J Exp Clin Cancer Res. 41:210. PubMed
  37. Ma S, et al. 2022. Nat Commun. 13:4118. PubMed
  38. González-Jiménez A, et al. 2022. Int J Mol Sci. 23: . PubMed
  39. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  40. Pereira PMR, et al. 2022. Nat Commun. 13:2526. PubMed
  41. Kwee BJ, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  42. Li Y, et al. 2022. J Med Virol. 94:3998. PubMed
  43. Busek M, et al. 2023. Lab Chip. 23:591. PubMed
  44. Pan R, et al. 2022. Cell. 185:1521. PubMed
  45. Im NG, et al. 2022. Cancer Immunol Res. 10:1055. PubMed
  46. Yang Y, et al. 2023. Front Immunol. 14:1033497. PubMed
RRID
AB_314043 (BioLegend Cat. No. 300307)
AB_314044 (BioLegend Cat. No. 300308)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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