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PE anti-human/mouse Granzyme B Recombinant Antibody

Pricing & Availability
Clone
QA16A02 (See other available formats)
Regulatory Status
RUO
Other Names
Granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-aseGranzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1, GZMB, CCP1, Asp-ase
Isotype
Mouse IgG1, κ
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Product Citations
publications
QA16A02_PE_GranzymeB_Antibody_1_042817
Human peripheral blood mononuclear cells were stained with CD8 Pacific Blue™, fixed and permeabilized, and then stained with Granzyme B PE (clone QA16A02, top) or mouse IgG1, κ PE isotype control (bottom).
  • QA16A02_PE_GranzymeB_Antibody_1_042817
    Human peripheral blood mononuclear cells were stained with CD8 Pacific Blue™, fixed and permeabilized, and then stained with Granzyme B PE (clone QA16A02, top) or mouse IgG1, κ PE isotype control (bottom).
  • QA16A02_PE_GranzymeB_Antibody_2_042817
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372207 25 tests $129
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372208 100 tests $335
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Description

Granzyme B is a 32 kD serine protease, also known as granzyme-2, serine protease B, CCP1, Asp-ase, and CTLA-1. Granzyme B is abundantly stored in the granules of cytotoxic T lymphocytes and NK cells. Low level of expression has been reported in granulocytes, B cells, and activated dendritic cells. Granzyme B is crucial for rapid induction of cell death and apoptosis through interaction with mannose-6-phosphate receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Human,Mouse
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Uchil PD et al. 2018. Cell host & microbe. 25(1):87-100 . PubMed
  2. Yeo L, et al. 2018. J Clin Invest. 128:3460. PubMed
  3. Rudd CE, et al. 2020. Cell Reports. 30(7):2075-2082. PubMed
  4. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  5. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  6. Zhang X, et al. 2020. Endocr Relat Cancer. 27:469. PubMed
  7. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  8. Nicolas-Boluda A, et al. 2021. eLife. 10:00. PubMed
  9. Li Z, et al. 2020. J Immunol. 2589:204. PubMed
  10. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  11. Liu X, et al. 2020. Nature. . PubMed
  12. Ostendorf BN, et al. 2020. Nat Med. 26:1048. PubMed
  13. Kim S, et al. 2020. Autophagy. :1. PubMed
  14. Alsaleh G, et al. 2020. Elife. 9: . PubMed
  15. Ruth JH, et al. 2021. JCI Insight. 6:e145662. PubMed
  16. Li ZY, et al. 2021. J Exp Med. 218:. PubMed
  17. Zhang L, et al. 2021. Mol Ther. 29:744. PubMed
  18. Yang X, et al. 2021. Front Pharmacol. 12:771046. PubMed
  19. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  20. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
  21. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  22. Chen L, et al. 2022. Mol Ther Oncolytics. 24:522. PubMed
  23. Hubrack S, et al. 2021. Sci Rep. 11:14090. PubMed
  24. Khurana P, et al. 2021. Front Immunol. 12:700374. PubMed
  25. Guo W, et al. 2022. J Immunother Cancer. 10:. PubMed
  26. Zhu S, et al. 2022. J Oncol. 2022:8724933. PubMed
  27. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
  28. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  29. Zhong G, et al. 2022. Oncoimmunology. 11:2114740. PubMed
  30. Yang Z, et al. 2022. J Immunother Cancer. 10:. PubMed
  31. Subham S, et al. 2022. Breast Cancer Res Treat. Online ahead of print. PubMed
  32. Taranto D, et al. 2021. Curr Protoc. 1:e147. PubMed
  33. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  34. Wang W, et al. 2022. Cell Rep. 41:111582. PubMed
  35. Liang Y, et al. 2022. Theranostics. 12:7729. PubMed
  36. Li J, et al. 2022. Front Immunol. 13:1043484. PubMed
  37. Lin J, et al. 2023. Nat Commun. 14:224. PubMed
  38. Jiang X, et al. 2023. Cell Discov. 9:7. PubMed
  39. Chen M, et al. 2023. Cell Biosci. 13:19. PubMed
  40. Khojandi N, et al. 2021. Cancer Immunol Res. 9:214. PubMed
  41. Allen SD, et al. 2021. Biomaterials. 269:120635. PubMed
  42. Cai Z, et al. 2023. Adv Sci (Weinh). 10:e2207155. PubMed
  43. Pan Y, et al. 2023. Adv Sci (Weinh). 10:e2206792. PubMed
  44. Lo WL, et al. 2023. Nat Immunol. 24:676. PubMed
  45. Wang X, et al. 2023. Nat Commun. 14:3440. PubMed
RRID
AB_2687031 (BioLegend Cat. No. 372207)
AB_2687032 (BioLegend Cat. No. 372208)

Antigen Details

Structure
32 kD serine protease
Distribution

Cytotoxic T cells, NK cells, and neutrophils, low on granulocytes, B cells and activated dendritic cells

Function
Granzyme B is able to induce target cell apoptosis by activating caspase independent pathways. Granzyme B is induced in CD8+ T lymphocytes with ConA/ IL-2 and CD4+ T lymphocytes with anti CD3/CD28 or CD3/CD46.
Interaction
Caspase-3
Ligand/Receptor
Mannose-6-phosphate receptor
Cell Type
T cells, NK cells, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Proteases, Enzymes and Regulators
Antigen References

1. Estebanez-Perpina E, et al. 2000. Biol Chem. 381:1203.
2. Griffiths GM. And S. Isaaz, et al. 1993. J. Cell Biol. 120:885.
3. Spaeny-Dekking EH, et al. 1998. J. Immunol. 160:3610.
4. Wagner C, et al. 2008. Mol. Immunol. 45:1761.

Gene ID
3002 View all products for this Gene ID 14939 View all products for this Gene ID
UniProt
View information about Granzyme B on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 05/01/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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