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PE/Cyanine7 anti-human CD14 Antibody

Pricing & Availability
Clone
M5E2 (See other available formats)
Regulatory Status
RUO
Workshop
III 329
Other Names
LPS receptor
Isotype
Mouse IgG2a, κ
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Product Citations
publications
M5E2_PECy7_061507
Human peripheral blood monocytes stained with M5E2 PE/Cyanine7
  • M5E2_PECy7_061507
    Human peripheral blood monocytes stained with M5E2 PE/Cyanine7
See PE/Cyanine7 spectral data
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301813 25 tests $143
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301814 100 tests $292
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens, and in the upregulation of adhesion molecules and expression of cytokines in monocytes and neutrophils.

Product Details
Technical data sheet

Product Details

Reactivity
Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length human CD14 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The M5E2 antibody inhibits monocyte activation and cytokine production induced by LPS. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, blocking of LPS stimulation4, and immunofluorescence microscopy5. Clone M5E2 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 301861 and 301862).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York. (IHC-F)
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  4. Power CP, et al. 2004. J. Immunol. 173:5229. (Block)
  5. Williams KC, et al. 2001. J. Exp. Med. 193:905.
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (FC) PubMed
  7. Santer DM, et al. 2010. J. Immunol. 485:4739. PubMed
  8. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  9. Zizzo G, et al. 2012. J. Immunol. 189:3508. PubMed
  10. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  11. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Subhi Y,et al. 2017. Invest Ophthalmol Vis Sci.. 58(12):5242-5250. PubMed
  2. Matlung HL et al. 2018. Cell reports. 23(13):3946-3959 . PubMed
  3. Sagebiel AF, et al. 2019. Nat Commun. 10:975. PubMed
  4. Crespo-Lessmann A, et al. 2017. J Asthma Allergy. 10:269. PubMed
  5. Rizzo MD, et al. 2019. J Neuroimmunol. 333:576969. PubMed
  6. Rizzo MD, et al. 2019. J Pharmacol Exp Ther. 371:191. PubMed
  7. Rizzo MD, et al. 2018. AIDS. 32:419. PubMed
  8. Cross S, et al. 2011. J Immunol. 187:5015. PubMed
  9. Zizzo G, et al. 2012. J Immunol. 189:3508. PubMed
  10. Spencer A, et al. 2014. PLoS One. 9:100538. PubMed
  11. Huang X, et al. 2020. Clin Transl Med. 10:e41. PubMed
  12. Bruveris FF, et al. 2020. Development. 147:00:00. PubMed
  13. Andreano E, et al. 2021. Cell. 184(7):1821-1835.e16. PubMed
  14. Cines DB, et al. 2020. Blood. 135:2085. PubMed
  15. Szentkereszty M, et al. 2020. Pathol Oncol Res. 26:1117. PubMed
  16. Andreano E, et al. 2021. Nature. Online ahead of print. PubMed
  17. Schimek V, et al. 2022. Cell Death Dis. 13:113. PubMed
  18. Pellett Madan R, et al. 2020. Clin Transplant. 34:e14021. PubMed
  19. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  20. Andreano E, et al. 2023. Nat Commun. 14:53. PubMed
  21. Olsen SN, et al. 2022. Mol Cell. 82:1140. PubMed
  22. Nguyen VVT, et al. 2022. J Extracell Vesicles. 11:e12280. PubMed
  23. Lütge M, et al. 2023. Nat Immunol. . PubMed
RRID
AB_389352 (BioLegend Cat. No. 301813)
AB_389353 (BioLegend Cat. No. 301814)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 4    Revision Date: 11/05/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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