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PE/Cyanine7 anti-human CD4 Antibody

Pricing & Availability
Clone
A161A1 (See other available formats)
Regulatory Status
RUO
Other Names
T4/Leu-3
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A161A1_PECy7_CD4_Antibody_FC_052815
Human peripheral blood lymphocytes were stained with CD4 (clone A161A1) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram).
  • A161A1_PECy7_CD4_Antibody_FC_052815
    Human peripheral blood lymphocytes were stained with CD4 (clone A161A1) PE/Cyanine7 (filled histogram) or rat IgG2b, κ PE/Cyanine7 isotype control (open histogram).
See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
357409 25 tests $153
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357410 100 tests $311
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Description

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD4 T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Product Citations
  1. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  2. Łyszkiewicz M, et al. 2020. Nat Commun. 11:1031. PubMed
  3. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  4. Wang K, et al. 2020. Signal Transduct Target Ther. 0.404861111. PubMed
  5. Corrado M, et al. 2020. Cell Metab. 32:981. PubMed
  6. Park JA, et al. 2020. J Hematol Oncol. 0.661111111. PubMed
  7. Faust HJ, et al. 2020. J Clin Invest. 130:5493. PubMed
  8. Park JA, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
  9. Le J, et al. 2020. Immunity. 52(6):1105-1118.e9. PubMed
  10. Hearnden R, et al. 2021. STAR Protocols. 2(2):100422. PubMed
  11. Mu Z, et al. 2021. Genome Biol. 122:22. PubMed
  12. Minns D, et al. 2021. Front Immunol. 12:633486. PubMed
  13. Luo Y, et al. 2021. Nat Commun. 12:3913. PubMed
  14. Wei JL, et al. 2021. J Immunother Cancer. 9: . PubMed
  15. Egedal JH, et al. 2021. Mucosal Immunol. 14:1203. PubMed
  16. Pekayvaz K, et al. 2022. Nat Commun. 13:1018. PubMed
  17. Kramer KJ, et al. 2022. Nat Commun. 13:3466. PubMed
  18. Su X, et al. 2022. J Transl Med. 20:378. PubMed
  19. Dai Z, et al. 2022. Signal Transduct Target Ther. 7:85. PubMed
  20. Hu Z, et al. 2022. Nat Metab. 4:759. PubMed
  21. Ma T, et al. 2022. Elife. 11: . PubMed
  22. Giannou AD, et al. 2023. Immunity. 56:125. PubMed
RRID
AB_2565661 (BioLegend Cat. No. 357409)
AB_2565662 (BioLegend Cat. No. 357410)

Antigen Details

Structure
55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution

Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells

Interaction
Part of the TCR/CD3 complex
Ligand/Receptor
β2 domain from the MHC class II
Cell Type
T cells, Monocytes, Macrophages, NKT cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
2. Vignali DA. 2010. J. Immunol. 184:5933.
3. Zhou L, et al. 2009. Immunity 30:646.
4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
5. Zhu J and Paul WE. 2008. Blood 112:1557.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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