Loading...

PerCP/Cyanine5.5 anti-human CD123 Antibody

Pricing & Availability
Clone
6H6 (See other available formats)
Regulatory Status
RUO
Other Names
IL-3Rα, IL-3 Receptor alpha
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
6H6_PerCPCy55_090208.jpg
Human peripheral blood lymphocytes stained with 6H6 PerCP/Cyanine5.5 and UCHL1 FITC
  • 6H6_PerCPCy55_090208.jpg
    Human peripheral blood lymphocytes stained with 6H6 PerCP/Cyanine5.5 and UCHL1 FITC
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
306015 25 tests $176
Check Availability


Need larger quantities of this item?
Request Bulk Quote
306016 100 tests $382
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD123 is the 70 kD transmembrane α chain of the IL-3 receptor. Alone, CD123 binds IL-3 with low affinity; when CD123 associates with CD131 (common β chain), it binds IL-3 with high affinity. CD123 does not transduce intracellular signals upon binding IL-3 and requires the β chain for this function. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, megakaryocytes, and some B cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-3Rα transfected COS cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Clone 6H6 does not inhibit IL-3 binding to low- or high-affinity IL-3Rs. Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation1, and immunohistochemical staining of acetone-fixed frozen sections2 and also paraformaldehyde fixed paraffin embedded tissue7.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Sun Q, et al. 1996. Blood 87:83. (IP, WB)
  2. Herling M, et al. 2003. Blood 101:5007. (IHC)
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Martin-Gayo E, et al. 2010. Blood 115:5366. PubMed
  5. Chen SC, et al. 2010. Arch Dermatol Res. 302:113. PubMed
  6. Liu Y, et al. 2012. Food Chem Toxicol. 50:1920. PubMed
  7. Peduzzi E, et al. 2007. J. Invest. Dermatol. 127:638. (IHC)
Product Citations
  1. Woolsey C et al. 2019. Cell Rep. 28(12):3032-3046 . PubMed
  2. Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed
  3. Manuel S, et al. 2013. AIDS Res Hum Retroviruses. 29:1273. PubMed
  4. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  5. Laing AG, et al. 2020. Nat Med. 26:1623. PubMed
  6. Jankeel A, et al. 2020. J Virol. :94. PubMed
  7. Li M, et al. 2020. Nat Commun. 4051:11. PubMed
  8. Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed
  9. Gu C, et al. 2021. Front Immunol. 12:678036. PubMed
  10. Morell M, et al. 2022. EBioMedicine. 76:103808. PubMed
  11. Sureshchandra S, et al. 2022. Cell Rep. 39:110938. PubMed
  12. Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
  13. Rhoades NS, et al. 2022. Cell Rep. 39:110725. PubMed
  14. Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed
  15. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  16. Ferrari B, et al. 2022. J Clin Invest. 132:. PubMed
  17. Li M, et al. 2023. Front Immunol. 14:1087923. PubMed
  18. Ait Djebbara S, et al. 2023. Front Immunol. 14:1180900. PubMed
RRID
AB_2124258 (BioLegend Cat. No. 306015)
AB_2264693 (BioLegend Cat. No. 306016)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, associates with CDw131, 70 kD
Distribution

Myeloid precursors, basophils, mast cells, macrophages, dendritic cells, megakaryocytes, subset of lymphocytes

Function
Hematopoietic cell proliferation, differentiation
Ligand/Receptor
IL-3
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, Macrophages, Mast cells, Megakaryocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Miyajima A, et al. 1993. Blood 82:1960.

Gene ID
3563 View all products for this Gene ID
UniProt
View information about CD123 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 04/18/2013

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account