Loading...

PerCP/Cyanine5.5 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
17A2_PerCPCyanine55_CD3_Antibody_020819_updated.png
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3 (clone 17A2) PerCP/Cyanine5.5 (left) or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • 17A2_PerCPCyanine55_CD3_Antibody_020819_updated.png
    C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3 (clone 17A2) PerCP/Cyanine5.5 (left) or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
100217 25 µg $118
Check Availability


Need larger quantities of this item?
Request Bulk Quote
100218 100 µg $323
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  2. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  3. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  4. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  5. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  6. Konishi Y, et al. 2018. iScience. 10:98. PubMed
  7. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  8. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  9. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  10. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  11. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  12. Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed
  13. Soutto M, et al. 2017. J Cancer. 8:2424. PubMed
  14. Agarwal P, et al. 2019. Cell Stem Cell. 24:769. PubMed
  15. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  16. Li SX, et al. 2019. PLoS Pathog. 15:e1007611. PubMed
  17. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  18. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  19. Riley JS, et al. 2020. Blood Adv. 4:1102. PubMed
  20. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  21. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  22. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  23. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  24. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  25. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  26. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  27. Molofsky A, et al. 2013. J Exp Med. 210:535. PubMed
  28. Al-Barwani F, et al. 2014. PLoS One. 9:104523. PubMed
  29. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  30. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  31. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  32. Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
  33. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  34. Tian Y, et al. 2016. J Immunol. 197: 1308 - 1321. PubMed
  35. Vidy A, et al. 2016. PLoS One. 11:e0165361. PubMed
  36. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  37. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  38. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  39. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  40. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  41. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  42. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  43. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  44. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  45. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  46. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  47. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  48. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  49. Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed
  50. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  51. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
  52. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  53. Chen Y, et al. 2020. Virol Sin. . PubMed
  54. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  55. Niderla-Bielinska J, et al. 2021. Int J Mol Sci. :22. PubMed
  56. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  57. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  58. Wang Y, et al. 2021. Front Immunol. 12:654463. PubMed
  59. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
  60. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  61. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  62. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
  63. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  64. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  65. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  66. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  67. Yang K, et al. 2022. J Clin Invest. 132:. PubMed
  68. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  69. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  70. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  71. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  72. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  73. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  74. Chen J, et al. 2022. J Nanobiotechnology. 20:283. PubMed
  75. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  76. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  77. Pishesha N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  78. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  79. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  80. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  81. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  82. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  83. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  84. Andrade ACDSP, et al. 2021. J Virol. 95:e0127621. PubMed
  85. Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  86. Colombo M, et al. 2022. iScience. 25:105042. PubMed
  87. Karanika S, et al. 2022. Front Immunol. 13:972266. PubMed
  88. Jin Q, et al. 2022. Drug Des Devel Ther. 16:3297. PubMed
  89. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  90. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  91. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  92. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  93. Wang H, et al. 2022. Immunol Cell Biol. 100:547. PubMed
  94. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  95. Rodrigues PB, et al. 2022. Cells. 11: . PubMed
  96. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  97. Choi H, et al. 2023. J Nanobiotechnology. 21:5. PubMed
  98. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  99. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  100. Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed
  101. Cui B, et al. 2023. Int Immunopharmacol. 114:109541. PubMed
  102. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  103. Dragan M, et al. 2022. J Invest Dermatol. 142:583. PubMed
  104. Alghamri MS, et al. 2022. ACS Nano. 16:8729. PubMed
  105. Lin J, et al. 2022. Adv Sci (Weinh). 9:e2202633. PubMed
  106. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  107. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  108. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  109. Aso K, et al. 2023. Nat Commun. 14:984. PubMed
  110. Wang Y, et al. 2023. EBioMedicine. 90:104480. PubMed
  111. Tan J, et al. 2023. Acta Pharm Sin B. 13:632. PubMed
  112. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
  113. Wiedmann L, et al. 2023. Nat Commun. 14:2353. PubMed
  114. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  115. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  116. Tsugawa H, et al. 2023. PLoS Pathog. 19:e1011139. PubMed
  117. Wang X, et al. 2023. Nat Commun. 14:3440. PubMed
  118. Zhou M, et al. 2023. Nat Commun. 14:3593. PubMed
  119. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  120. Guo X, et al. 2023. Mol Immunol. 160:32. PubMed
RRID
AB_1595597 (BioLegend Cat. No. 100217)
AB_1595492 (BioLegend Cat. No. 100218)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 3    Revision Date: 02/08/2019

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account