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Purified anti-human CD326 (EpCAM) Antibody

Pricing & Availability
Clone
9C4 (See other available formats)
Regulatory Status
RUO
Other Names
Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
Isotype
Mouse IgG2b, κ
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Product Citations
publications
9C4_Pure_020708
Human colon carcinoma cell line HT29 stained with purified 9C4, followed by anti-mouse IgG FITC
  • 9C4_Pure_020708
    Human colon carcinoma cell line HT29 stained with purified 9C4, followed by anti-mouse IgG FITC
  • 9C4_Purified_CD326_Antibody_IF_112012
    BT474 breast cancer cells were stained with anti-human CD326 (clone 9C4) using 1:100 dilution, followed by DyLight™ 649 anti-mouse Ig secondary antibody (red) plus DAPI staining for nuclei (blue). Cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, blocked with 10% serum, and incubated O/N at 4°C. Images were taken under 20x (filter set: EX 647/10x, Dichroic 665LP, EM 700/70x) at exposure 4s. Data provided by John Nolan and Er Liu, La Jolla Bioengineering Institute.
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324201 25 µg $101
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324202 100 µg $206
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Description

CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. Recently, it has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
DU.4475 breast carcinoma
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
IHC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the revelant formats) include: immunofluorescence, immunohistochemistry3, and spatial biology (IBEX)4,5.

Application References

(PubMed link indicates BioLegend citation)
  1. Lammers R, et al. 2002. Exp. Hematol. 30:537.
  2. Schultz LD, et al. 2010. P. Natl. Acad. Sci. USA 107:13022. PubMed
  3. Human Protein Atlas http://www.proteinatlas.org/ENSG00000119888/antibody (IHC)
  4. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  5. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hutt M, et al. 2018. Oncotarget. 9:11322. PubMed
  2. Hinohara K, et al. 2018. Cancer Cell. 34:939. PubMed
  3. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  4. Genga RMJ et al. 2019. Cell reports. 27(3):708-718 . PubMed
  5. Huerta–Nuñ ez LFE, et al. 2019. Sci Rep. 9:6419. PubMed
  6. Ding L, et al. 2019. Nat Commun. 10:4182. PubMed
  7. Gee MH, et al. 2018. Cell. 172:549. PubMed
  8. Kim J, et al. 2018. J Histochem Cytochem. 66:879. PubMed
  9. Salerno–Goncalves R, et al. 2019. PLoS Negl Trop Dis. 13:e0007650. PubMed
  10. Valès S, et al. 2019. EBioMedicine. 49:172. PubMed
  11. Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
  12. Davies SP, et al. 2019. Cell Rep. 29:1610. PubMed
  13. Batth IS, et al. 2020. BMC Cancer. 20(1):715. PubMed
  14. Ruiz–Riquelme A, et al. 2018. Acta Neuropathol Commun. 6:26. PubMed
  15. Kaneko MK, et al. 2020. Oncol Rep. 44:2517. PubMed
  16. Morsing M, et al. 2020. Breast Cancer Res. 0.9875. PubMed
  17. Yildizhan Y, et al. 2021. J Extracell Vesicles. e12059:10. PubMed
  18. Krishnan V, et al. 2020. Gut. 69:1738. PubMed
  19. Shi G, et al. 2022. Methods Mol Biol. 2394:591. PubMed
  20. Poggi A, et al. 2022. Curr Protoc. 2:e366. PubMed
  21. de Vargas Roditi L, et al. 2022. Cell Rep Med. 3:100604. PubMed
  22. Njock MS, et al. 2022. J Extracell Vesicles. 11:e12228. PubMed
  23. Goh D, et al. 2022. Front Immunol. 13:939989. PubMed
  24. Ramos SA, et al. 2022. J Allergy Clin Immunol. 149:767. PubMed
  25. Leader AM, et al. 2021. Cancer Cell. 39:1594. PubMed
  26. Li G, et al. 2023. Int J Mol Med. 51: . PubMed
  27. Peterson HM, et al. 2022. Adv Sci (Weinh). 9:e2200415. PubMed
  28. Wang J, et al. 2023. ACS Sens. 8:1200. PubMed
  29. Yildizhan Y, et al. 2023. Int J Mol Sci. 24:. PubMed
  30. Qin XY, et al. 2023. Cell Death Dis. 14:358. PubMed
RRID
AB_756075 (BioLegend Cat. No. 324201)
AB_756076 (BioLegend Cat. No. 324202)

Antigen Details

Structure
Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
Distribution

Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.

Function
Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
Modification
Glycosylated.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Strnad J, et al. 1989. Cancer Res. 49:314.
2. Munz M, et al. 2004. Oncogene 23:5748.
3. Rao CG, et al. 2005. Int. J. Oncol. 27:49.

Gene ID
4072 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org
Go To Top Version: 2    Revision Date: 01/14/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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