Loading...

Purified anti-human CD9 Antibody

Pricing & Availability
Clone
HI9a (See other available formats)
Regulatory Status
RUO
Workshop
V P018
Other Names
Tetraspanin, MRP-1, DRAP-24
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
HI9a
Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC
  • HI9a
    Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC
  • HI9a_PURE_CD9_Antibody_ICC_011921
    BT474 breast cancer cell line was stained with anti-human CD9, detected with anti-mouse DyLight™ 649, and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute
Cat # Size Price Quantity Check Availability Save
312102 100 µg $112
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Dörsam B, et al. 2018. Front Immunol. 7:e34961. PubMed
  2. Bachurski D, et al. 2019. J Extracell Vesicles. 8:1596016. PubMed
  3. Temoche‐Diaz MM et al. 2019. Elife. 8 pii: e47544. PubMed
  4. Klymiuk MC, et al. 2019. BMC Vet Res. 15:42. PubMed
  5. Kretschmann S, et al. 2019. J Clin Invest. 130:2952. PubMed
  6. Gunasekaran M, et al. 2020. J Heart Lung Transplant. 39:379. PubMed
  7. Hasselmann J, et al. 2020. Neuron. 103(6):1016-1033. PubMed
  8. McQuade A, et al. 2020. Nat Commun. 4.1875. PubMed
  9. Fitzgerald W, et al. 2018. Am J Reprod Immunol. 80:e12860. PubMed
  10. Nakai W, et al. 2016. Sci Rep. 6: 33935. PubMed
  11. Olivia S Chao et al. 2017. Journal of cellular biochemistry. 118(12):4414-4424 . PubMed
  12. Zhang X, et al. 2020. Int J Mol Sci. 21:00. PubMed
  13. Zhang X, et al. 2020. J Extracell Vesicles. 9:1791450. PubMed
  14. Layton TB, et al. 2020. Nat Commun. 2.380555556. PubMed
  15. Dobrowolski M, et al. 2020. Development. 147:00:00. PubMed
  16. Lu-Culligan A, et al. 2021. Med. 2(5):591-610.e10. PubMed
  17. Lobastova L, et al. 2021. Frontiers in Cell and Developmental Biology. 9:698503. PubMed
  18. Wagner M, et al. 2020. Nat Commun. 1147:11. PubMed
  19. Arab T, et al. 2021. J Extracell Vesicles. 10:e12079. PubMed
  20. Araki Y, et al. 2021. Front Oncol. 11:667109. PubMed
  21. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  22. Kawakami K, et al. 2021. Sci Rep. 11:15000. PubMed
  23. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  24. Zabegina L, et al. 2021. Cells. 10:. PubMed
  25. Valkov N, et al. 2021. Life Sci Alliance. 4:. PubMed
  26. Yao PJ, et al. 2021. Biomedicines. 9:. PubMed
  27. Dobrowolski M, et al. 2020. Development. . PubMed
  28. Kobayashi Y, et al. 2021. Front Physiol. 12:693007. PubMed
  29. NULL, et al. 2022. Cell. 185:916. PubMed
  30. Khan AA, et al. 2022. Bioconjug Chem. . PubMed
  31. Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed
  32. Li H, et al. 2020. Haematologica. 105:1206. PubMed
  33. Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed
  34. Sayeed N, et al. 2022. PLoS One. 17:e0272511. PubMed
  35. Zheng W, et al. 2022. Nat Biomed Eng. 6:979. PubMed
  36. Fordjour FK, et al. 2022. J Biol Chem. :102394. PubMed
  37. Claes C, et al. 2021. Mol Neurodegener. 16:50. PubMed
  38. Andriessen A, et al. 2022. Vet Comp Oncol. 20:381. PubMed
  39. Bitirim CV, et al. 2022. Sci Rep. 12:5651. PubMed
  40. Chernyshev VS, et al. 2022. J Extracell Vesicles. 11:e12256. PubMed
  41. Desjardins P, et al. 2022. Int J Mol Sci. 23:. PubMed
  42. Alpert A, et al. 2022. Cell Syst. 13:71. PubMed
  43. Lenzini S, et al. 2021. ACS Nano. 15:17439. PubMed
  44. Layton TB, et al. 2022. Proc Natl Acad Sci U S A. 119:e2120336119. PubMed
  45. Glass MC, et al. 2022. Cell Rep. 39:110728. PubMed
  46. Haag F, et al. 2022. Cells. 11: . PubMed
  47. Claes C, et al. 2022. Alzheimers Dement. 18:1765. PubMed
  48. Ciftci Dede E, et al. 2023. PLoS One. 18:e0282238. PubMed
  49. Powell BH, et al. 2023. Int J Mol Sci. 24:. PubMed
RRID
AB_314907 (BioLegend Cat. No. 312102)

Antigen Details

Structure
Tetraspan family, type III transmembrane protein, 24 kD
Distribution

Platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial and epithelial cells

Function
Adhesion, platelet activation, B cell development
Ligand/Receptor
Associates with CD63, CD81, CD82 and CD36, binds PSG17
Cell Type
B cells, Embryonic Stem Cells, Endothelial cells, Epithelial cells, Granulocytes, Lymphocytes, Platelets
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Miao WM, et al. 2001. Blood 97:1689.
2. Ellerman DA, et al. 2003. Mol Biol Cell. 14:5098.
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.

Gene ID
928 View all products for this Gene ID
UniProt
View information about CD9 on UniProt.org
Go To Top Version: 3    Revision Date: 01/19/2021

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account