- Clone
- DECMA-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- E-Cadherin, Cadherin-1, CDH1, and UVO
- Isotype
- Rat IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![DECMA1_Purified_CD324_Antibody_FC_011714 DECMA1_Purified_CD324_Antibody_FC_011714](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/DECMA_Purified_CD324_Antibody_FC_011714.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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MDCK epithelial cell line was stained with purified CD324 (clone DECMA-1, filled histogram) or purified rat IgG1, κ isotype control (open histogram), followed by anti-rat IgG PE. -
Canine kidney cell line MDCK was cultured in a chamber slide till confluent. The cells were fixed with 1% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 30 minutes. Then cells were intracellularly stained with purified 2.5 µg/ml of CD324 (E-Cadherin, clone DECMA-1) in blocking buffer overnight at 4°C followed by 2.5 µg/ml of DyLight™ 594 Goat anti-rat IgG (minimal x-reactivity) incubation for 1 hour at 4°C. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with 40X objective.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
147301 | 25 µg | $124 | ||||
147302 | 100 µg | $247 |
CD324, also known as E-cadherin, cadherin-1, CDH1, and UVO is a member of the cadherin superfamily. It is a calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region. CD324 is widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells. CD324 functions as a cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. In bacterial pathogenesis, the ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization. CD324 binds to the αEβ7 integrin to mediate cell adhesion and also interacts with a number of intracellular proteins including including erbin, ezrin, caspase-3, caspase-8, β-catenin, presenilin 1, and casein kinase II as well as other extracellular proteins including the EGF receptor.
Product DetailsProduct Details
- Reactivity
- Mouse,Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- E-Cadherin extracellular domain
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC - Verified
IP, WB, FA - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
It is also recommended using EDTA-based solutions for dissociating attachment-dependent cell lines. - Application Notes
-
Additional reported applications (for relevant formats) include: immunoprecipitation1, Western Blotting1, immunomicroscopy3, biological function1,2, and spatial biology (IBEX)4,5.
-
Application References
(PubMed link indicates BioLegend citation) -
- Vestweber D, et al. 1985. EMBO. 4:3393. (IP, WB, FA)
- Nakagawa M, et al. 2001. J. Cell Sci. 114:1829. (FA in canine cells)
- Mohamet L, et al. 2010. PLoS ONE. 5:e12921. (IF)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
- RRID
-
AB_2563037 (BioLegend Cat. No. 147301)
AB_2563038 (BioLegend Cat. No. 147302)
Antigen Details
- Structure
- Member of the cadherin superfamily. Calcium-dependent, transmembrane cell-cell adhesion glycoprotein composed of four extracellular cadherin repeats and a highly conserved cytoplasmic tail region.
- Distribution
-
Widely expressed in epithelial cells in the colon, uterus, liver, keratinocytes, brain, heart, muscle, kidney, and pancreas as well as erythroid cells.
- Function
- Cell adhesion molecule involved in development, bacterial pathogenesis, and tumor invasion. The ectodomain of CD324 mediates bacterial adhesion to mammalian cells, while the cytoplasmic domain is required for internalization.
- Interaction
- Interacts with a variety of proteins including erbin, ezrin, caspase-3, caspase-8, EGF receptor, β-catenin, presenilin 1, casein kinase II, and others.
- Ligand/Receptor
- αEβ7 integrin.
- Cell Type
- Embryonic Stem Cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Stem Cells, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Overduin M, et al. 1995. Science 267:386.
2. Boggon TJ, et al. 2002. Science 296:1308.
3. Berx G, et al. 1995. EMBO J. 14:6107.
4. Perl AK, et al. 1998. Nature 392:190. - Gene ID
- 999 View all products for this Gene ID 12550 View all products for this Gene ID
- UniProt
- View information about CD324 on UniProt.org
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