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Purified anti-mouse IL-17A Antibody

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
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Product Citations
publications
mIL-17A_040408
  • mIL-17A_040408
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506901 50 µg $106
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506902 500 µg $282
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Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
CyTOF® - Verified
ELISPOT Capture, ICFC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-17 recombinant protein ranging from 1000 to 15.63 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
  8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
  9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
  10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
  11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
  12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
  13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
  14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
  15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Zhang S, et al. 2017. Nature.. 10.1038/nature24283. PubMed
  2. Oyler‐Yaniv J et al. 2017. Molecular cell. 66(5):635-647 . PubMed
  3. Chen Y, et al. 2017. J Autoimmun. 77:96. PubMed
  4. He Z, et al. 2017. J Immunol. 199:955. PubMed
  5. Volchenkov R, et al. 2017. Front Immunol. 0.818055556. PubMed
  6. Toubai T, et al. 2017. Blood Adv. 1.095138889. PubMed
  7. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  8. Kaisar MMM, et al. 2018. PLoS Biol. 16:e2005504. PubMed
  9. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  10. Abboud G, et al. 2018. Front Immunol. 9:1973. PubMed
  11. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  12. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  13. Mencarelli A, et al. 2018. Front Immunol. 9:261. PubMed
  14. Yeola AP, et al. 2020. Front Immunol. 10:3115. PubMed
  15. Hua J, et al. 2018. Sci Rep. 5.235416667. PubMed
  16. Karmaus PWF, et al. 2019. Nature. 565:101. PubMed
  17. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  18. Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
  19. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
  20. Smith E, et al. 2008. J Immunol. 181:1357. PubMed
  21. Jordan J, et al. 2008. Infect Immun. 76:3717. PubMed
  22. Cooney L, et al. 2011. J Immunol. 187:4440. PubMed
  23. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  24. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  25. Fu Y, et al. 2020. Mol Ther. 1214:28. PubMed
  26. Wang Y, et al. 2021. Front Immunol. 11:601601. PubMed
  27. Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed
  28. Takeuchi Y, et al. 2021. Sci Rep. 11:18679. PubMed
  29. Muhammad F, et al. 2020. J Autoimmun. 111:102441. PubMed
  30. Paterson MJ, et al. 2020. PLoS Pathog. 16:e1008733. PubMed
  31. Nguyen H, et al. 2020. Clin Cancer Res. 26:3481. PubMed
  32. Yang J, et al. 2020. Front Immunol. 10:3048. PubMed
  33. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  34. Cardoso F, et al. 2021. Nature. 597:410. PubMed
  35. Guo R, et al. 2020. Cell Res. 30:21. PubMed
  36. Yoon S, et al. 2020. Antioxidants (Basel). 10:. PubMed
  37. Kästele V, et al. 2021. Mucosal Immunol. 14:717. PubMed
  38. Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed
  39. Landuyt AE, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  40. Yan J, et al. 2020. Cancer Discov. 10:124. PubMed
  41. Yu H, et al. 2020. Front Immunol. 11:1773. PubMed
  42. Altemus J, et al. 2022. J Cell Physiol. 237:3001. PubMed
  43. Sato A, et al. 2022. Biol Pharm Bull. 45:1798. PubMed
RRID
AB_315461 (BioLegend Cat. No. 506901)
AB_315462 (BioLegend Cat. No. 506902)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.
Go To Top Version: 4    Revision Date: 06/14/2019

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