- Clone
- QE5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- HNUP153, N153, Nuclear Pore Complex Protein NUP153, Nucleoporin Nup153, 153 kD nucleoporin, nuclear pore complex protein hnup153
- Previously
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Covance Catalog# MMS-102P
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QE5_Purified_NUP153_Antibody_1_IHC_022415 QE5_Purified_NUP153_Antibody_1_IHC_022415](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QE5_Purified_NUP153_Antibody_IHC_022415.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Methanol fixed HeLa stained with the antibody QE5. This antibody brilliantly highlights the nuclear membrane (green). The golgi is stained with the antibody to Giantin. -
Immunofluorescence of HeLa cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B) NUP153 Mouse primary antibody (Clone QE5). 2 μg/ml (1:250 dilution) Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time is 300 ms. Concentrations 4 µg/ml. -
Total lysates (15 µg protein) from Daudi, HEL (Human), COS-7 (Monkey), Raw264.7 (Mouse), UMR106 (Rat) and CHO (Hamster) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (1 µg/ml) purified NUP153 antibody, clone QE5. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-&betal-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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906201 | 100 µL | $253 |
Nucleoporin 153 (Nup153) is a component of the nuclear pore complex, and is required for the anchoring of the nuclear pore complex to the nuclear pore membrane. Nup153 has a critical role in the nuclear export of proteins and RNA, and is involved in the retention of unspliced mRNAs in the nucleus.
Product DetailsProduct Details
- Reactivity
- Human,Mouse,Rat,Non-Human Primate,Hamster
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The QE5 monoclonal antibody was generated against rat liver nuclear envelope proteins.
- Formulation
- Phosphate-buffered solution + 0.03% thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
ICC - Quality tested
WB, IP - Verified
IEM - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration of 4.0 μg/ml (1:250 dilution) is recommended. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml (1:500-1:1000 dilution). For immunoprecipitation, the suggested use of this reagent is 1:50 dilution. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting, immunocytochemistry (ICC) and immunoprecipitation (IP).
*Predicted MW = 250 kD
This antibody recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern.
Predicted MW is ~ 250 kD, observed MW is ~154 kD. Lower bands (~ 60 kD) maybe the tripartite structure of NUP153, which consists of a zinc finger domain flanked by large (60-70 kD) NH2- and COOH-terminal domains9.
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Application References
(PubMed link indicates BioLegend citation) -
- Pare GC, et al. 2005. Exp Cell Res. 303:388.
- N Pante, et al. 1994. J Cell Biol 126:603.
- Smythe C, et al. 2000. EMBO J. 19:3918. (WB)
- Goujon C, et al. 2008. J Virol. 82:12335. (IF) PubMed
- Holzenspies JJ, et al. 2009. BMC Dev Biol.9:8. (IF) PubMed
- Green JE, et al. 2014. Dev Biol. 392:419. (IF) PubMed
- Chen CY, et al. 2012. Cell. 149:565. (IF, WB)
- Iborra FJ, et al.2000. J Cell Sci. 113:291. (IEM)
- Bastos R, et al. 1997. J Cell Biol. 137:989. (IF, IP)
- Product Citations
- RRID
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AB_2565060 (BioLegend Cat. No. 906201)
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Nuclear Markers
- Gene ID
- 9972 View all products for this Gene ID
- UniProt
- View information about NUP153 on UniProt.org
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