Biotin anti-human IL-5 Antibody

Pricing & Availability
Clone
JES1-5A10 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-5, Eosinophil differentiation factor (EDF), Eosinophil colony stimulating factor (Eo-CSF), B cell growth factor-2 (BCGF-2), B cell differentiation factor for IgM (BCDF-m), IgA enhancing factor (IgA-EF), T cell replacing factor-1 (TRF-1)
Isotype
Rat IgG2a, κ
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Product Citations
publications
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501002 500 µg $274
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Description

IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. Monomeric human IL-5 is a 126 amino acid protein with a reported molecular weight of 26 kD for the homodimeric protein. Mouse and human IL-5 are approximately 70% identical. IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. The JES1-39D10 antibody can neutralize the bioactivity of natural or recombinant IL-5.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
COS-expressed, recombinant human IL-5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of human IL-5 protein ranging from 250 to 1 pg/ml are recommended for each ELISA plate when paired with TRFK5 (Cat. # 504302). To obtain a linear standard curve, serial dilutions of human IL-5 protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate when paired with JES1-39D10 (Cat. # 500902).
For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA1-3 or ELISPOT4-6 Detection: The purified JES1-5A10 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated JES1-39D10 (Cat. No. 501002) as the detection antibody. Each lot of this antibody is quality control tested by ELISA assay.
Neutralization1: The LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-5 bioactivity.
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining8,9 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-5 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430401 & 430404) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5.
  2. Butterfield J, et al. 1992. Blood 79:688.
  3. Abrams J, et al. 1994. Eosinophils in Allergy and Inflammation. Marcel Dekker New York. p.133.
  4. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19.6.
  5. Mahanty S, et al. 1992. J. Immunol. 148:3567.
  6. Limaye A, et al. 1991. J. Clin. Inv. 88:1418.
  7. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
  8. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  9. Andersson J, et al. 1994. Immunology 83:16.
Product Citations
  1. Keumatio Doungtsop BC, et al. 2024. J Allergy Clin Immunol Glob. 100193:3. PubMed
  2. Yan J, et al. 2020. Cell Rep. 107820:31. PubMed
RRID
AB_315144 (BioLegend Cat. No. 501002)

Antigen Details

Structure
Cytokine; dimer; 45 kD (Mammalian)
Bioactivity
Growth/differentiation of eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
Eosinophils
Receptors
Heterodimer IL-5Rα (CD125); β-subunit (CDw131) in common with IL-3R, GM-CSFR
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Takatsu K, et al. 1988. Immunol. Rev. 102:107.
3. Takatsu K. 1992. Curr. Opin. Immunol. 4:299.
4. Takatsu K. 1991. Microbiol. Immun. 35:593.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
3567 View all products for this Gene ID
UniProt
View information about IL-5 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All IL-5 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human IL-5 JES1-5A10 ELISA Detection,ELISPOT Detection
Go To Top Version: 2    Revision Date: 12/14/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-human IL-5

    Human_IL-5_020405
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