- Clone
- RI7217 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Transferrin receptor, T9
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RI7217_ULEAF_CD71_Antibody_051018 RI7217_ULEAF_CD71_Antibody_051018](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RI7217_ULEAF_CD71_Antibody_051018.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse bone marrow cells were stained with Ultra-LEAF™ purified CD71 (clone RI7217, left) or purified rat IgG2a, κ isotype control (right), followed by anti-rat IgG FITC and then Ter-119 APC. Data shown was gated on total bone marrow cells.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
113821 | 100 µg | $182 | ||||
113822 | 1 mg | $578 |
Select size of product is eligible for a 40% discount! Promotion valid until September 30, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.
CD71 is a 95 kD type II heterodimeric transmembrane glycoprotein that is also known as T9 and transferrin receptor. CD71 is expressed on proliferating cells, reticulocytes, and erythroid precursors. Its expression is very low on resting leukocytes. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking of cellular proliferation. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 113821 & 113822).
This clone may also be known as R17217 or R17 217. -
Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_2734171 (BioLegend Cat. No. 113821)
AB_2734172 (BioLegend Cat. No. 113822)
Antigen Details
- Structure
- Transferrin receptor and is 95 kD.
- Distribution
-
Most dividing cells, the brain endothelium, and cycling hematopoietic progenitors.
- Function
- Mediates iron uptake.
- Ligand/Receptor
- Transferrin.
- Cell Type
- Hematopoietic stem and progenitors
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Hentze MW, et al. 1996. P. Natl. Acad. Sci. USA 93:8175.
2. Trowbridge IS, et al. 1993. Annu. Rev. Cell Biol. 9:129.
3. Trowbridge I, et al. 1982. J. Cell Physiol. 112:403.
4. Kuhn LC, et al. 1984. Cell 37:95. - Gene ID
- 22042 View all products for this Gene ID
- UniProt
- View information about CD71 on UniProt.org
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
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BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
-
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD71 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-mouse CD71 | RI7217 | FC |
FITC anti-mouse CD71 | RI7217 | FC |
PE anti-mouse CD71 | RI7217 | FC |
Purified anti-mouse CD71 | RI7217 | FC |
PE/Cyanine7 anti-mouse CD71 | RI7217 | FC |
Brilliant Violet 421™ anti-mouse CD71 | RI7217 | FC |
PerCP/Cyanine5.5 anti-mouse CD71 | RI7217 | FC |
APC anti-mouse CD71 | RI7217 | FC |
Ultra-LEAF™ Purified anti-mouse CD71 | RI7217 | FC |
PE/Dazzle™ 594 anti-mouse CD71 | RI7217 | FC |
Brilliant Violet 510™ anti-mouse CD71 | RI7217 | FC |
TotalSeq™-A0441 anti-mouse CD71 | RI7217 | PG |
TotalSeq™-C0441 anti-mouse CD71 | RI7217 | PG |
APC/Fire™ 750 anti-mouse CD71 | RI7217 | FC |
TotalSeq™-B0441 anti-mouse CD71 | RI7217 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ter-119 AP... -
FITC anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ter-119 AP... -
PE anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ter-119 AP... -
Purified anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ter-119 AP... -
PE/Cyanine7 anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with TER-119 AP... -
Brilliant Violet 421™ anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ter-119 AP... -
PerCP/Cyanine5.5 anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ter-119 AP... -
APC anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ter-119 PE... -
Ultra-LEAF™ Purified anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ultra-LEAF... -
PE/Dazzle™ 594 anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ter-119 Al... -
Brilliant Violet 510™ anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with Ter-119 PE... -
TotalSeq™-A0441 anti-mouse CD71
-
TotalSeq™-C0441 anti-mouse CD71
-
APC/Fire™ 750 anti-mouse CD71
C57BL/6 mouse bone marrow cells were stained with anti-mouse... -
TotalSeq™-B0441 anti-mouse CD71
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