- Clone
- JES3-19F1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin 10, B cell derived T cell growth factor (B-TCGF), Cytokine synthesis inhibitory factor (CSIF), T-cell growth inhibitory factor (TGIF)
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![JES3-19F1_PE_IL-10_Antibody_1_100416 JES3-19F1_PE_IL-10_Antibody_1_100416](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/JES3-19F1_PE_IL-10_Antibody_1_100416.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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LPS-stimulated (in the presence of monensin, 18 hours) human peripheral blood mononuclear cells were surface stained with CD14 APC, then fixed with Fixation Buffer permeabilized with Permeabilization Wash Buffer and intracellularly stained with anti-human IL-10 (clone JES3-19F1) PE (top) or rat IgG2a, κ PE isotype control (bottom). Data shown was gated on monocyte population. -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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506804 | 100 tests | 172€ |
IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-19F1 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-19F1 antibody can neutralize the bioactivity of natural or recombinant IL-10.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- COS-expressed recombinant human IL-10
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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ELISA or ELISPOT Capture1-4: The Purified JES3-19F1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated JES3-12G8 antibody (Cat. Nos. 501502 & 501503) as the detecting antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture. For use as an ELISPOT capture antibody, a concentration range of 4.0 - 8.0 µg/ml is recommended.
Flow Cytometry: The fluorochrome-labeled JES3-19F1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-10 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,2: The Ultra-LEAF™ Purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-10 bioactivity (Cat. Nos. 506814 & 506815).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining5,6 of paraformaldehyde-fixed, saponin-treated frozen tissue sections, and immunocytochemistry.
Note: For testing human IL-10 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430601 to 430606) are specially developed and recommended. - Application References
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- Abrams J, et al. 1992. Immunol. Rev. 127:5.
- Gotlieb W, et al. 1992. Cytokine 4:385.
- Yssel H, et al. 1992. J. Immunol. 149:2378.
- Burdin N, et al. 1993. J. Exp. Med. 177:295.
- Andersson U, et al. 1999. Detection and quantification of gene expression. New York: Springer-Verlag.
- Andersson J, et al. 1994. Immunology 83:16.
- Product Citations
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- RRID
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AB_315454 (BioLegend Cat. No. 506804)
Antigen Details
- Structure
- Acid-labile cytokine; dimer; 35-40 kD (Mammalian)
- Bioactivity
- Inhibit IFN-γ, TNF-β, IL-2 production by TH1 clones; inhibits macrophage-mediated IL-1, IL-6, TNF-α synthesis; suppress delayed type hypersensitivity response; stimulate TH2 cell response; mast cell proliferation in
- Cell Sources
- Activated CD8+ and CD4+ T cells, activated monocytes, mast cells, Ly-1 B (mouse)
- Cell Targets
- T cells, B cells, mast cells, macrophages
- Receptors
- IL-10R (CDw210)
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. de Waal-Malefyt R, et al. 1992. Curr. Opin. Immunol. 4:314.
3. Howard M, et al. 1992. Immunol. Today. 13:198.
4. Quesniaux V. 1992. Research Immunol. 143:385. - Regulation
- Production inhibited by IL-4, IL-10
- Gene ID
- 3586 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- IL-10
- Specificity Alt (DOES NOT SHOW ON TDS):
- IL-10
- App Abbreviation (DOES NOT SHOW ON TDS):
- ICFC
- UniProt
- View information about IL-10 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All IL-10 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-human IL-10 | JES3-19F1 | ICFC |
PE anti-human IL-10 | JES3-19F1 | ICFC |
Purified anti-human IL-10 | JES3-19F1 | ELISA Capture,IHC-F,ICC,WB |
PE/Dazzle™ 594 anti-human IL-10 | JES3-19F1 | ICFC |
Ultra-LEAF™ Purified anti-human IL-10 | JES3-19F1 | ELISA Capture,IHC-F,ICC,WB |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human IL-10
LPS-stimulated human peripheral blood mononuclear cells (PBM... -
PE anti-human IL-10
LPS-stimulated (in the presence of monensin, 18 hours) human... -
Purified anti-human IL-10
-
PE/Dazzle™ 594 anti-human IL-10
LPS-stimulated (in the presence of monensin, 18 hours) human... -
Ultra-LEAF™ Purified anti-human IL-10
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