- Clone
- 100 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B-Cell CLL/Lymphoma 2 (Bcl-2), Protein Phosphatase 1, Regulatory Subunit 50
- Isotype
- Mouse IgG1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![100_Bcl-2_Purified_Antibody_WB_121313.jpg 100_Bcl-2_Purified_Antibody_WB_121313.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/100_Bcl-2_Purified_Antibody_WB_121313.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Western blot analysis of extracts from THP-1(lane 1), PBMC(lane 2) and Raw264.7(lane 3) cells using anti-Bcl-2 antibody(clone100). β-actin was used as loading control. -
IHC staining using purified anti-Bcl-2 antibody (clone 100) on formalin-fixed paraffin-embedded human tonsil tissue. Following antigen retrieval using 0.1 M Tris-buffered saline with Tween 20 (Cat. No. 925501), the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 647 goat anti-mouse IgG (yellow) (Cat. No. 405322) for one hour at room temperature. Nuclei were counterstained with DAPI (blue), and the slide was mounted with ProLong™ Gold Antifade Mountant. The image was captured with a 40x objective. Scale bar: 50 µm
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
658701 | 25 µg | 90€ | ||||
658702 | 100 µg | 165€ |
Bcl-2 is a conserved anti-apoptotic protein that plays important roles in normal immunity. It forms homodimers or heterodimers with other Bcl-2 family members. This protein regulates apoptosis through controlling mitochondrial fusion and fission. Phosphorylation of Bcl-2 has been shown to enhance activity to allow response to extracellular growth-factor-mediated signals. Bcl-2 is also regulated by IRES, miR-15a, miR-16-1 and RNA-BP nucleolin. Chromosome 18q21.3 translocation and overexpression of Bcl-2 is frequently observed in follicular lymphomas and some diffuse large B-cell lymphomas.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide of amino acids 41-54 of human bcl-2 oncoprotein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application References
- Product Citations
-
- RRID
-
AB_2562958 (BioLegend Cat. No. 658701)
AB_2562959 (BioLegend Cat. No. 658702)
Antigen Details
- Structure
- Two isoforms, 239 or 205 amino acids in length, with a predicted molecular weight of 26 kD or 22 kD and four ‘BH' domains.
- Distribution
-
Outer mitochondrial membrane, nuclear membrane, endoplasmic reticulum membrane.
- Function
- Regulates apoptosis through controlling mitochondrial fusion and fission.
- Interaction
- BAX, BAD, BAK, Bcl-X(L),EI24,APAF1, BBC3, BCL2L1, BNIPL, MRPL41, TP53BP2, FKBP8 BAG1, RAF1, EGLN3, G0S2, and BOP.
- Cell Type
- B cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, Immunology, Mitochondrial Function, Neuroscience, Signal Transduction, Transcription Factors, Ubiquitin/Protein Degradation
- Antigen References
-
1. Lin P, et al. 2013. Curr. Hematol. Malig. Rep. 8:243.
2. Tomita N. 2011. J. Clin. Exp. Hematop. 51:7.
3. Kelly PN, et al. 2011. Cell Death Differ. 18:1414.
4. Willimott S, et al. 2010. Biochem. Soc. Trans. 38:1571.
5. Rolland SG, et al. 2010. Curr. Opin. Cell Biol. 22:852. - Gene ID
- 596 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Bcl-2
- Specificity Alt (DOES NOT SHOW ON TDS):
- Bcl-2
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB,IHC-F,IHC-P
- UniProt
- View information about Bcl-2 on UniProt.org
Other Formats
View All Bcl-2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Bcl-2 | 100 | WB,IHC-F,IHC-P |
Alexa Fluor® 647 anti-Bcl-2 | 100 | ICFC,SB |
Alexa Fluor® 488 anti-Bcl-2 | 100 | ICFC |
PE anti-Bcl-2 | 100 | ICFC |
Brilliant Violet 421™ anti-Bcl-2 | 100 | ICFC |
TotalSeq™-Bn1180 anti-Bcl-2 | 100 | SB |
TotalSeq™-B1180 anti-Bcl-2 | 100 | ICPG |
Customers Also Purchased
![](/Files//Images/media_assets/products/product_images/2D2_Purified_Bax_Antibody_1_031620.png)
![](/Files//Images/media_assets/products/product_images/BC96_Pure_071805.jpg)
![](/Files//Images/media_assets/products/product_images/7F11A10_TCF1_Purified_Antibody_1_WB_061418_updated.png)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-Bcl-2
Western blot analysis of extracts from THP-1(lane 1), PBMC(l... IHC staining using purified anti-Bcl-2 antibody (clone 100) ... -
Alexa Fluor® 647 anti-Bcl-2
Human peripheral blood lymphocytes were treated with Fixatio... Confocal image of human lymph node sample acquired using the... -
Alexa Fluor® 488 anti-Bcl-2
Human peripheral blood lymphocytes were treated with Fixatio... -
PE anti-Bcl-2
Human peripheral blood lymphocytes were treated with Fixatio... -
Brilliant Violet 421™ anti-Bcl-2
Human peripheral blood lymphocytes were treated with Fixatio... -
TotalSeq™-Bn1180 anti-Bcl-2
-
TotalSeq™-B1180 anti-Bcl-2
Follow Us