- Clone
- W20064D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Forkhead Box O1, FOXO1A, FKH1, FKHR
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![W20064D_Alexa-Fluor-647_FOXO1_Antibody_102522 W20064D_Alexa-Fluor-647_FOXO1_Antibody_102522](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/W20064D_Alexa-Fluor-647_FOXO1_Antibody_102522-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Daudi cells (positive control) (filled histogram) and MOLT-4 cells (negative control) (open histogram) were fixed and permeabilized using True Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with Alexa Fluor® 647 anti-FOXO1 (clone W20064D), or Alexa Fluor® 647 rat IgG2b, κ isotype control (open histogram, dashed line) (representative histogram for both cell lines) (Cat. No. 400626).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
947205 | 25 tests | 132€ | ||||
947206 | 100 tests | 300€ |
Forkhead box protein O1 (FOXO1) is a member of the Forkhead box class O family of transcription factors. As such, it is involved in the regulation of a wide range of vital cellular processes including cell cycle progression, differentiation, proliferation, DNA repair, stress response, and apoptosis. FOXO1 is activated via serine and threonine phosphorylation. Phosphorylation is triggered by external stimuli including growth factors, cytokines, and insulin and can be PKB/Akt-mediated as part of the P13K-Akt/SGK pathway on Thr24, Ser256, and Ser319, mediated by CDK2 or CK1 on Ser249 and Ser322/325, respectively, or induced by serum and glucocorticoid-inducible kinase or NFkB kinase. Upon phosphorylation, FOXO1, which is localized to the nucleus, translocates to the cytoplasm following binding to 14-3-3 proteins. In addition to phosphorylation, FOXO1 can be activated via acetylation of its lysine residues by coactivator p300. FOXO1 is vital to the regulation of metabolism through the promotion of glucose homeostasis and gluconeogenic enzyme expression, adipose tissue and skeletal muscle formation, and oxidative stress regulation. FOXO1 may inhibit cell cycle progression and induce apoptosis in cancer cells.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human FOXO1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
This clone was tested for ICC using serum-starved HeLa cells treated with insulin and untreated serum-starved HeLa cells. Staining was expected to localize to the nucleus prior to treatment and localize to the cytoplasm following treatment. Three fix/perm methods were used: methanol fixation or 4% PFA fixation followed by permeabilization with either methanol or Triton X-100. Only PFA fixation followed by methanol permeabilization produced a strong signal and the expected shift in localization.
This clone was tested for IHC on human melanoma tissue using Biolegend’s DAB Chromagen. No staining was produced. - RRID
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AB_2927910 (BioLegend Cat. No. 947205)
AB_2927910 (BioLegend Cat. No. 947206)
Antigen Details
- Structure
- FOXO1 is a 655 amino acid protein with a predicted molecular weight of 69 kD.
- Distribution
-
Ubiquitously expressed /Nucleus and Cytoplasm
- Function
- Transcription Factor
- Interaction
-
RET signaling: PDGF, EGFR, DAP12, MAPK, NGF
Cytokine Receptor Gamma-Chain Family signaling: IL-21, IL-7, IL-9, IL-15, IL-2, IL-4, adipokines, insulin, thrombopoietin
GAB1 signalosome: PI3K/AKT, GAB1
Immune response IL-23 signaling: Tie2
- Cell Type
- B cells, Tregs
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Immunology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
- Greer E and Brunet A. 2005. Oncogene. 24:7410.
- Gross D, et al. 2008. Oncogene. 27:2320.
- Perrot V and Matthew R. 2005. Mol. Endocrinol. 19:2283.
- Tzivion G, et al. 2011. BBA. 1813:1938.
- Yang Y, et al. 2005. EMBO. 24:1021.
- Gene ID
- 2308 View all products for this Gene ID
- UniProt
- View information about FOXO1 on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All FOXO1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-FOXO1 Antibody | W20064D | WB,ICC,ICFC |
PE anti-FOXO1 | W20064D | ICFC |
Alexa Fluor® 647 anti-FOXO1 | W20064D | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-FOXO1 Antibody
Whole cell extracts (15 µg total protein) from HEL, MOLT4 (n... Daudi cells (filled histogram, positive control) and MOLT-4 ... ICC staining of purified anti-FOXO1 antibody (clone W20064D)... -
PE anti-FOXO1
Daudi cells (positive control) (filled histogram) and MOLT-4... -
Alexa Fluor® 647 anti-FOXO1
Daudi cells (positive control) (filled histogram) and MOLT-4...
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