- Clone
- A17012A (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Signal Transduced Activator of Transcription 1, Transcription Factor ISGF-3 components p91/p84
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A17012A_A647_STAT1_Antibody_1_080519_updated.png A17012A_A647_STAT1_Antibody_1_080519_updated.png](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A17012A_A647_STAT1_Antibody_1_080519_updated.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were treated with (left) or without (right) Recombinant Human IFN-γ (carrier-free, Cat. No. 570204) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with CD4 PE and anti-STAT1 Phospho (Tyr701) (clone A17012A) Alexa Fluor® 647. Data was gated on lymphocyte and monocyte populations. -
Human peripheral blood monocytes were treated with (filled histogram) or without (open histogram) Recombinant Human IFN-γ (carrier free, Cat. No. 570204) for 15 minutes, fixed with Fixation Buffer (Cat. No. 420801), permeabilized with True-Phos™ Perm Buffer (Cat. No. 425401), then stained with anti-STAT1 Phospho (Tyr701) (clone A17012A) Alexa Fluor® 647.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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666409 | 25 tests | 113€ | ||||
666410 | 100 tests | 296€ |
STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated at Tyrosine 701 (Tyr701) by receptor-associated kinases, including JAK1, JAK2, and TYK2. This results in STAT1 dimerization and subsequent translocation to the nucleus, where it functions as a transcriptional activator. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Clone A17012A recognizes STAT1 phosphorylated at Tyrosine 701 (Tyr701).
When using this clone for ICC, we recommend using methanol to permeabilize fixed cells. - RRID
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AB_2814502 (BioLegend Cat. No. 666409)
AB_2814503 (BioLegend Cat. No. 666410)
Antigen Details
- Structure
- Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
- Distribution
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Ubiquitous tissue expression, nucleoplasmic-cytosolic distribution
- Function
- Immune response activation, cell signaling
- Interaction
- STAT1, STAT2, IRF9, ERBB4, JAK1, JAK2, TYK2, TNK1, SHP2
- Biology Area
- Cell Biology
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
-
- Moretti S, et al. 2017. J. Biol. Chem. 292: 1785.
- Wei J, et al. 2015. J. Immunol. 195: 2870.
- Sung PS, et al. 2015. Proc. Natl. Acad. Sci. USA. 112: 10443
- Ooi EL, et al. 2014. Proc. Natl. Acad. Sci. USA. 111: 1909.
- Wu TR, et al. 2002. J. Biol. Chem. 277: 47572.
- Horvath, et al. 1996. J. Virol. 70: 647.
- Haque SJ, et al. 1995. J. Biol. Chem. 270: 25709.
- Gene ID
- 6772 View all products for this Gene ID
- UniProt
- View information about STAT1 Phospho Tyr701 on UniProt.org
Other Formats
View All STAT1 Phospho (Tyr701) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-STAT1 Phospho (Tyr701) | A17012A | WB,ICFC,ICC,ChIP |
PE anti-STAT1 Phospho (Tyr701) | A17012A | ICFC |
Alexa Fluor® 647 anti-STAT1 Phospho (Tyr701) | A17012A | ICFC |
PerCP/Cyanine5.5 anti-STAT1 Phospho (Tyr701) | A17012A | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-STAT1 Phospho (Tyr701)
Total cell lysates (15 µg protein) from serum-starved HeLa S... Total cell lysates (15 µg protein) from serum-starved HeLa S... Human peripheral blood mononuclear cells were treated with (... Human peripheral blood monocytes were treated with (filled h... Serum starved HeLa cells were untreated (panel A) or stimula... Chromatin Immunoprecipitations (ChIP) were performed with cr... -
PE anti-STAT1 Phospho (Tyr701)
Human peripheral blood mononuclear cells were treated with (... Human peripheral blood monocytes were treated with (filled h... -
Alexa Fluor® 647 anti-STAT1 Phospho (Tyr701)
Human peripheral blood mononuclear cells were treated with (... Human peripheral blood monocytes were treated with (filled h... -
PerCP/Cyanine5.5 anti-STAT1 Phospho (Tyr701)
Human peripheral blood mononuclear cells were treated with (... Human peripheral blood monocytes were treated with (filled h...
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