- Clone
- 5-271 (See other available formats)
- Other Names
- GPIIIb, GPIV
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![5-271_GMP_APCFire750_CD36_Antibody_111523 5-271_GMP_APCFire750_CD36_Antibody_111523](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/5-271_GMP_APCFire750_CD36_Antibody_111523.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Typical results from human peripheral blood platelets stained either with 5-271 APC/Fire™ 750 used at 5 µL/test (filled histogram) or with an isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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260356 | 100 tests | 287€ |
CD36 is an 85 kD integral membrane glycoprotein, also known as GPIIIb, or GPIV. It is expressed on various epithelial and endothelial cells as well as erythrocytes, platelets, macrophages/monocytes and some macrophage-derived dendritic cells. CD36 functions as a scavenger receptor, binding thrombospondin, long chain fatty acids, oxidized LDL, collagen type I, IV, and V as well as apoptotic cells. The 5-271 antibody has been reported to be useful for flow cytometry.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human platelets
- Formulation
- Phosphate-buffered solution, pH 7.2, containing True-Stain Monocyte Blocker™, 0.09% sodium azide and 0.2% (w/v) BSA (origin USA) and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- 100 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
- Excitation Laser
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Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunofluorescence2.
- Application References
-
- Stelner E, et al. 2006. J. Cell Sci. 119:459.
- Stewart DA, et al. 2012. Mol. Cancer Res. 10:727. (IF)
- Disclaimer
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GMP RUO Flow Cytometry Antibodies. BioLegend GMP RUO fluorophore conjugated antibodies are manufactured in a dedicated GMP facility and compliant with ISO 13485:2016. For research use only. Not for use in diagnostic or therapeutic procedures. Our processes include:
- Batch-to-batch consistency
- Material traceability
- Documented procedures
- Documented employee training
- Equipment maintenance and monitoring records
- Lot-specific certificates of analysis
- Quality audits per ISO 13485:2016
- QA review of released products
Antigen Details
- Structure
- Integral membrane protein, CD36 family, single chain glycoprotein, 85-113 kD
- Distribution
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Expressed in various epithelial and endothelial cells, erythrocytes, platelets, monocytes/ macrophages, some macrophage-derived dendritic cells
- Function
- Scavenger receptor for thrombospondin, long chain fatty acids, anionic phospholipids, oxidized LDL, collagen types I, IV and V. Involved in recognition and phagocytosis of apoptotic cells, cell adhesion.
- Ligand/Receptor
- Thrombospondin, anionic phospholipids, oxidized LDL, collage types I, IV, and V
- Cell Type
- Dendritic cells, Endothelial cells, Epithelial cells, Erythrocytes, Macrophages, Monocytes, Platelets
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
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1. Hogg N, et al. 1984. Immunology 53-753.
2. Greenwalt DE, et al. 1992. Blood 80:1105.
3. Armsesilla AL, et al.1994. J. Biol. Chem. 269:18985.
4. Endemann G, et al. 1993. J. Biol. Chem. 268:11811. - Gene ID
- 948 View all products for this Gene ID
- UniProt
- View information about CD36 on UniProt.org
Related FAQs
Other Formats
View All CD36 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD36
Human peripheral blood platelets stained with purified 5-271... Human frozen tonsil tissue slice was stained with purified a... -
APC anti-human CD36
Human peripheral blood platelets stained with 5-271 APC -
APC/Cyanine7 anti-human CD36
Human peripheral blood platelets stained with 5-271 APC/Cyan... -
FITC anti-human CD36
Human peripheral blood platelets stained with 5-271 FITC -
PE anti-human CD36
Human peripheral blood platelets were stained with CD36 (clo... -
Purified anti-human CD36 (Maxpar® Ready)
Human PBMCs stained with 154Sm-anti-CD45 (HI30) and 152Sm-an... -
Biotin anti-human CD36
Human peripheral blood platelets were stained with biotinyla... -
APC/Fire™ 750 anti-human CD36
Human peripheral blood platelets were stained with CD36 (clo... -
PE/Cyanine7 anti-human CD36
Human peripheral blood platelets were stained with CD36 (clo... -
PerCP/Cyanine5.5 anti-human CD36
Human peripheral blood platelets were stained with CD36 (clo... -
TotalSeq™-A0407 anti-human CD36
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TotalSeq™-C0407 anti-human CD36
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Brilliant Violet 421™ anti-human CD36
Human peripheral blood platelets were stained with CD36 (clo... -
Alexa Fluor® 488 anti-human CD36
Human peripheral blood platelets were stained with CD36 (clo... -
TotalSeq™-B0407 anti-human CD36
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Alexa Fluor® 700 anti-human CD36 Antibody
Human peripheral blood platelets were stained with anti-huma... -
TotalSeq™-D0407 anti-human CD36
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FITC anti-human CD36
Typical results from human peripheral blood platelets staine... -
APC/Fire™ 750 anti-human CD36
Typical results from human peripheral blood platelets staine... -
GMP FITC anti-human CD36
Typical results from human peripheral blood platelets staine... -
GMP APC/Fire™ 750 anti-human CD36
Typical results from human peripheral blood platelets staine... -
Spark YG™ 581 anti-human CD36
Human peripheral blood platelets were stained with anti-huma... -
Spark Red™ 718 anti-human CD36 (Flexi-Fluor™)
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Spark Blue™ 550 anti-human CD36
Human peripheral blood platelets were stained with anti-huma... -
PE/Fire™ 640 anti-human CD36
Human peripheral blood platelets were stained with anti-huma...
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