- Clone
- 5C3/Parkin (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PARK2, AR-JP, LPRS2, PDJ, PRKN, E3 ubiquitin-protein ligase parkin, Parkin 2, E3 ubiquitin ligase, parkinson disease protein 2, parkinson juvenile disease protein 2, Parkinson disease (autosomal recessive, juvenile) 2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![5C3-Parkin_Purified_Parkin_Antibody_1_050919 5C3-Parkin_Purified_Parkin_Antibody_1_050919](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/5C3-Parkin_Purified_Parkin_Antibody_1_050919.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
-
Western blot of purified anti-Parkin antibody (clone 5C3/Parkin). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
Western blot of purified anti-Parkin antibody (clone 5C3/Parkin). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate. The blot was incubated with 2.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
865601 | 25 µg | 85€ | ||||
865602 | 100 µg | 221€ |
Parkin (also known as PARK2, AR-JP, PRKN) is a protein encoded by the PARK2 gene in humans. It is a component of the E3 ubiquitin ligase complex that mediates the targeting of proteins for degradation. Mutations in the PARK2 gene cause a familial form of Parkinson’s disease called autosomal recessive juvenile Parkinson’s disease (AR-JP).
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2810777 (BioLegend Cat. No. 865601)
AB_2810778 (BioLegend Cat. No. 865602)
Antigen Details
- Structure
- Parkin is a 465 amino acid protein with an apparent molecular mass of ~ 52 kD.
- Distribution
-
Tissue Distribution: Brain, heart, testis, and skeletal muscle.
Cellular Distribution: Cytosol, mitochondria, nucleus, and endoplasmic reticulum. - Function
- Parkin in involved in protein ubiquitination, protein degradation, mitochondria function, and neurodegeneration.
- Interaction
- Ubiquitin, PINK1
- Ligand/Receptor
- BCL2, SYT11, CCNE1, GPR37, RHOT1/MIRO1, MFN1
- Cell Type
- Astrocytes, Microglia, Neurons, Oligodendrocytes
- Biology Area
- Cell Biology, Mitochondrial Function, Neurodegeneration, Neuroinflammation, Protein Misfolding and Aggregation, Ubiquitin/Protein Degradation
- Molecular Family
- Enzymes and Regulators, Ligases
- Antigen References
-
- Tay SP, et al. 2018. J Biol Chem. 285: 29231-29238.
- Pawlyk AC, et al. 2003. J Biol Chem. 278(48):48120-8.
- Gene ID
- 5071 View all products for this Gene ID
- UniProt
- View information about Parkin on UniProt.org
Related FAQs
Other Formats
View All Parkin Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Parkin | 5C3/Parkin | WB |
Customers Also Purchased
![](/Files//Images/media_assets/products/product_images/A15165D_PURE_Parkin_Antibody_1_120219.png)
![](/Files//Images/media_assets/products/product_images/A15165E_PURE_Parkin_Antibody_1_121819.png)
![](/Files//Images/media_assets/products/product_images/Prk8_Purified_Parkin_Antibody_1_031919.png)
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Follow Us