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Alexa Fluor® 555 Goat anti-mouse IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4053 (See other available formats)
Regulatory Status
RUO
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly4053_A555_IgG_Antibody_031521
HUVEC cells were fixed with 1% paraformaldehyde (PFA), and then stained with 10 µg/ml of purified CD144 (VE-Cadherin) (clone BV9) at 4°C overnight, followed by 2.5 µg/ml of Alexa Fluor® 555 anti-mouse IgG (yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured by 20X objective.
  • Poly4053_A555_IgG_Antibody_031521
    HUVEC cells were fixed with 1% paraformaldehyde (PFA), and then stained with 10 µg/ml of purified CD144 (VE-Cadherin) (clone BV9) at 4°C overnight, followed by 2.5 µg/ml of Alexa Fluor® 555 anti-mouse IgG (yellow) for 2 hours at room temperature. Nuclei were counterstained with DAPI and are shown in blue. The image was captured by 20X objective.
See Alexa Fluor® 555 spectral data
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405324 100 µg 90€
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Description

This polyclonal goat anti-mouse IgG antibody reacts with the heavy chains of mouse IgG and with the light (kappa and lambda) chains common to most mouse immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 555 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 2.5 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 555 has an excitation maximum of 555 nm, and a maximum emission of 565 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

This polyclonal goat anti-mouse IgG antibody is useful for capture or detection of mouse IgG in ELISA.

Application References
  1. Scotta C, et al. 2008. J Immunol. 181:1025-33. PubMed
  2. Gao X, et al. 2014. Cell Mol Neurobiol. 34:257-68 (ICC)
  3. Winters T, et al. 2014. EMBO J. 33:1256-70.
Product Citations
  1. Kishore M et al. 2017. Immunity. 47(5):875-889 . PubMed
  2. Biering SB et al. 2017. Cell host & microbe. 22(1):74-85 . PubMed
  3. Fuchs R, et al. 2015. Toxicology. 338: 17-29. PubMed
  4. Iannitti R, et al. 2016. Nat Commun. 7:10791. PubMed
  5. Webber M, et al. 2016. Proc Natl Acad Sci U S A. 113(50):14189-14194. PubMed
  6. Divito SJ, et al. 2020. J Clin Invest. 130:4624. PubMed
  7. Meyers TA, et al. 2020. Hum Mol Genet. 944:29. PubMed
  8. van de Veerdonk FL, et al. 2021. J Clin Invest. Online ahead of print. PubMed
  9. Della Marina A, et al. 2021. Cells. 10:. PubMed
  10. Zhu L, et al. 2021. Pharmaceutics. 14:. PubMed
  11. Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
  12. Feher A, et al. 2022. Sci Rep. 12:22023. PubMed
  13. Deisher A, et al. 2022. STAR Protoc. 3:101663. PubMed
RRID
AB_2563179 (BioLegend Cat. No. 405324)
Go To Top Version: 3    Revision Date: 03.15.2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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