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Alexa Fluor® 647 anti-mouse CD31 Antibody

Pricing & Availability
Clone
390 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
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Product Citations
publications
390_A647_CD31_Antibody_1_080607
C57BL/6 mouse splenocytes stained with 390 Alexa Fluor® 647
  • 390_A647_CD31_Antibody_1_080607
    C57BL/6 mouse splenocytes stained with 390 Alexa Fluor® 647
  • 390_A647_CD31_Antibody_2_053018
    C57BL/6 mouse frozen liver section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of anti-mosue CD31 (clone 390) Alexa Fluor® 647 (red) overnight at 4°C. Nuclei were counterstained with DAPI (green). The image was captured by 10X objective.
See Alexa Fluor® 647 spectral data
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102415 25 µg 81€
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102416 100 µg 184€
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1) and EndoCAM. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation and angiogenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C3H/HeJ mouse hematopoietic progenitor cell line 3
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining5,6,7 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Special Note: This antibody is not recommended for formalin-fixed paraffin-embedded sections. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102412).

Application References
  1. Baldwin HS, et al. 1994. Development 120:2539. (IP, Block)
  2. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  3. Rosenblum WI, et al. 1996. Stroke 27:709. (Block)
  4. Iguchi A, et al. 1997. Cell Struct. Funct. 22:357. (Block)
  5. Wyder L, et al. 2000. Cancer Res. 60:4682. (IHC)
  6. Wiewrodt R, et al. 2002. Blood 99:912. (IHC)
  7. McQualter JL, et al. 2009. Stem Cells. 27:623. (IHC) PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
Product Citations
  1. Degn SE et al. 2017. Cell. 170(5):913-926 . PubMed
  2. Jagannathan P, et al. 2017. Sci Rep. 10.1038/s41598-017-10624-3. PubMed
  3. Park JK, et al. 2018. Stem Cell Res Ther. 9:197. PubMed
  4. Fan MY et al. 2018. Cell reports. 25(5):1204-1213 . PubMed
  5. Moretti FA, et al. 2018. Elife. 7:e35816. PubMed
  6. Firl DJ et al. 2018. eLife. 7 pii: e33051. PubMed
  7. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  8. Qi Y, et al. 2019. Sci Adv. 5:eaau8355. PubMed
  9. Akram KM, et al. 2019. Nat Commun. 10:1178. PubMed
  10. Dougan M, et al. 2018. Cancer Immunol Res. 6:389. PubMed
  11. Chojnacki A, et al. 2019. Commun Biol. 2:181. PubMed
  12. Esposito M, et al. 2019. Nat Cell Biol. 21:627. PubMed
  13. Lui BG, et al. 2020. Nat Commun. 0.663194444. PubMed
  14. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  15. Zhu J, et al. 2020. Adv Sci (Weinh). 7:1903185. PubMed
  16. Plant T, et al. 2020. J Clin Invest. 130:3221. PubMed
  17. Graney PL, et al. 2020. Sci Adv. 6:eaay6391. PubMed
  18. Michael BD, et al. 2020. Cell Reports. 32(11):108150. PubMed
  19. Pauken KE, et al. 2020. Cell Reports. 31(13):107827. PubMed
  20. McQualter J, et al. 2009. Stem Cells. 27:623. PubMed
  21. Riedel S, et al. 2012. PLoS One. 7:e52398. PubMed
  22. Berdnikovs S, et al. 2013. Am J Physiol Lung Cell Mol Physiol. 304:240. PubMed
  23. Mori Y, et al. 2014. Sci Rep. 4:6997. PubMed
  24. Soveg F, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L573-L583. PubMed
  25. Stalin J, et al. 2016. Oncogene. 10.1038/onc.2016.83. PubMed
  26. Miyawaki T, et al. 2020. Nat Commun. 1.225. PubMed
  27. Roncato F, et al. 2021. Cell Adhesion Migration. 15(1):166-179. PubMed
  28. Neupane AS, et al. 2020. Cell. 183(1):110-125.e11. PubMed
  29. Huang S, et al. 2020. Cell. 184(2):441-459.e25. PubMed
  30. Dudeck J, et al. 2021. Immunity. 54(3):468-483.e5. PubMed
  31. Prizant H, et al. 2021. Cell Reports. 36:109523. PubMed
  32. Matryba P, et al. 2020. J Immunol. 1395:204. PubMed
  33. Oni TE, et al. 2020. J Exp Med. :217. PubMed
  34. Cong L, et al. 2021. Breast Cancer Res. 23:51. PubMed
  35. Wan P, et al. 2021. Neurophotonics. 8:035007. PubMed
  36. De Niz M, et al. 2021. Cell Rep. 36:109741. PubMed
  37. Shanahan MT, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G668. PubMed
  38. Hsu HP, et al. 2021. J Biol Chem. 296:100419. PubMed
  39. Lüönd F, et al. 2022. STAR Protoc. 3:101438. PubMed
  40. Zotter B, et al. 2022. J Neurosci. 42:183. PubMed
  41. De Niz M, et al. 2022. STAR Protoc. 3:101450. PubMed
  42. Matryba P, et al. 2020. J Biophotonics. 13:e202000072. PubMed
  43. Liu L, et al. 2022. Elife. 11: . PubMed
  44. Zhu J, et al. 2022. Neurophotonics. 9:045008. PubMed
  45. Lambert AW, et al. 2022. Dev Cell. 57:2714. PubMed
  46. Li Y, et al. 2023. Theranostics. 13:403. PubMed
  47. Zhu J, et al. 2023. Cell Rep Methods. 3:100407. PubMed
RRID
AB_493411 (BioLegend Cat. No. 102415)
AB_493410 (BioLegend Cat. No. 102416)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org
Go To Top Version: 5    Revision Date: 05.30.2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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