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Alexa Fluor® 700 anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
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Product Citations
publications
UCHT1_Alx700_080408.jpg
Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 700
  • UCHT1_Alx700_080408.jpg
    Human peripheral blood lymphocytes stained with UCHT1 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
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300423 25 µg 101€
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300424 100 µg 202€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD3 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed
  2. Keustermans G,et al. 2017. PLoS One. 10.1371/journal.pone.0187068. PubMed
  3. Chen J, et al. 2017. Sci Rep. 10.1038/s41598-017-11056-9. PubMed
  4. Hansen IS, et al. 2018. Nat Commun. 9:863. PubMed
  5. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
  6. Roberta Zappasodi et al. 2018. Cancer cell. 33(6):1017-1032 . PubMed
  7. Ivan Jelcic et al. 2018. Cell. 175(1):85-100 . PubMed
  8. Rousset F, et al. 2018. Mol Ther Nucleic Acids. 14:351. PubMed
  9. Leclerc M, et al. 2019. Nat Commun. 10:3345. PubMed
  10. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  11. Berry MR et al. 2017. Cell. 170(5):860-874 . PubMed
  12. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  13. Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed
  14. Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
  15. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  16. Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
  17. Bochem J, et al. 2019. PLoS One. 14:e0221301. PubMed
  18. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  19. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  20. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  21. Chiu YL, et al. 2018. Immun Ageing. 15:27. PubMed
  22. Na I, et al. 2013. Haematologica. 98:23. PubMed
  23. Laprevotte E, et al. 2013. Leuk Res. 37:440. PubMed
  24. Zhou J, et al. 2015. J Immunol. 194:4688. PubMed
  25. Mashiko S, et al. 2015. J Allergy Clin Immunol. 136: 351-359. PubMed
  26. Sundström P, et al. 2015. J Immunol. 195: 3472 - 3481. PubMed
  27. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  28. Khaitan A, et al. 2016. PLoS One. 11: 0161786. PubMed
  29. Kiniry B, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.100. PubMed
  30. Chandele A, et al. 2016. J Virol. 90(24):11259-11278. PubMed
  31. Hood SP, et al. 2020. Elife. 9:00. PubMed
  32. Walsh RE, et al. 2020. MAbs. 12:1764829. PubMed
  33. Corgnac S, et al. 2021. STAR Protocols. 2(1):100267. PubMed
  34. Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
  35. Golebski K, et al. 2021. Immunity. 54(2):291-307.e7. PubMed
  36. Cheng Y, et al. 2021. Immunity. 54(8):1825-1840.e7. PubMed
  37. OConnor RA, et al. 2021. OncoImmunology. 10(1):1940675. PubMed
  38. Goc J, et al. 2021. Cell. 184:5015. PubMed
  39. Chen Y, et al. 2020. Cell. 1496:183. PubMed
  40. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  41. FitzPatrick MEB, et al. 2021. Cell Rep. 34:108661. PubMed
  42. Lutter L, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1567. PubMed
  43. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  44. Khuzwayo S, et al. 2021. Front Immunol. 12:631410. PubMed
  45. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  46. Harris LD, et al. 2020. Front Cell Infect Microbiol. 10:120. PubMed
  47. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  48. Janssen JJE, et al. 2022. Am J Physiol Endocrinol Metab. 322:E141. PubMed
  49. Yoshida R, et al. 2022. Cancer Res. :. PubMed
  50. Studniberg SI, et al. 2022. Mol Syst Biol. 18:e10824. PubMed
  51. Jacobs ME, et al. 2021. Immunother Adv. 1:ltaa004. PubMed
  52. Kim JH, et al. 2020. Sci Rep. 10:1835. PubMed
  53. Chen Y, et al. 2022. Sci Immunol. 7:eabp8328. PubMed
  54. Labuz DR, et al. 2023. Elife. 12: . PubMed
  55. Tan GMY, et al. 2022. Cancers (Basel). 14:. PubMed
  56. Xiong H, et al. 2023. EBioMedicine. 90:104507. PubMed
  57. Menevse AN, et al. 2023. Acta Neuropathol Commun. 11:75. PubMed
RRID
AB_493740 (BioLegend Cat. No. 300423)
AB_493741 (BioLegend Cat. No. 300424)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 2    Revision Date: 06.27.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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