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Alexa Fluor® 700 anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
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Product Citations
publications
N418_Alx700_080408.jpg
C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 700 and M5/114.15.2 PE
  • N418_Alx700_080408.jpg
    C57BL/6 mouse splenocytes stained with N418 Alexa Fluor® 700 and M5/114.15.2 PE
See Alexa Fluor® 700 spectral data
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117319 25 µg 76€
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117320 100 µg 172€
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Krementsov DN, et al. 2018. Front Immunol. 9:1622. PubMed
  2. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  3. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  4. Li X, et al. 2018. Cell Host Microbe. 24:847. PubMed
  5. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  6. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  7. An YA et al. 2017. eLife. 6 pii: e24071. PubMed
  8. Mishra BB, et al. 2017. Nat Microbiol. 2:17072. PubMed
  9. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  10. Wu H, et al. 2019. Autophagy. :1. PubMed
  11. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  12. Nadafi R, et al. 2020. Cell Reports. 30(12):4110-4123. PubMed
  13. Atibalentja D, et al. 2011. J Immunol. 186:1421. PubMed
  14. Sarkar J, et al. 2013. Invest Ophthalmol Vis Sci . 54:5920. PubMed
  15. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  16. Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
  17. Xiang W, et al. 2020. Signal Transduct Target Ther. 0.374305556. PubMed
  18. Hreha TN, et al. 2020. Front Immunol. 1.597916667. PubMed
  19. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  20. Sadiq BA, et al. 2020. Current Protocols in Immunology. 131(1):e115. PubMed
  21. Dammeijer F, et al. 2020. Cancer Cell. 38(5):685-700.e8. PubMed
  22. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  23. Doron I, et al. 2021. Cell. 184(4):1017-1031.e14. PubMed
  24. Hassan AO, et al. 2021. Cell Reports. 36(4):109452. PubMed
  25. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  26. Wiesner DL, et al. 2020. Cell Host Microbe. 614:27. PubMed
  27. Lim CX, et al. 2020. Cell Rep. 3793:30. PubMed
  28. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
  29. Panea C, et al. 2021. Commun Biol. 4:913. PubMed
  30. Khan KA, et al. 2020. NPJ Breast Cancer. 6:29. PubMed
  31. Hickey A, et al. 2021. Front Microbiol. 12:653587. PubMed
  32. França TT, et al. 2021. JCI Insight. 6:. PubMed
  33. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  34. Golden TN, et al. 2022. Front Pharmacol. 12:761496. PubMed
  35. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  36. Castellanos CA, et al. 2021. Sci Immunol. 6:eabh0707. PubMed
  37. Dourcy M, et al. 2020. Mucosal Immunol. 13:799. PubMed
  38. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  39. Bell CR, et al. 2022. Nat Commun. 13:2063. PubMed
  40. Zhu K, et al. 2022. EMBO Rep. 23:e54499. PubMed
  41. Yang N, et al. 2022. NPJ Vaccines. 7:120. PubMed
  42. Knizkova D, et al. 2022. Nat Immunol. 23:1644. PubMed
  43. Leonardi I, et al. 2022. Cell. 185:831. PubMed
  44. MacPherson KP, et al. 2023. Front Cell Neurosci. 17:895017. PubMed
RRID
AB_528735 (BioLegend Cat. No. 117319)
AB_528736 (BioLegend Cat. No. 117320)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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