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APC anti-human CD107a (LAMP-1) Antibody

Pricing & Availability
Clone
H4A3 (See other available formats)
Regulatory Status
RUO
Workshop
P PR-63; BP 473; P P008
Other Names
Lysosome-Associated Membrane Protein 1, LGP-120, LAMP-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
H4A3_APC_110308.jpg
Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) APC (pink histogram) or mouse IgG1, κ APC (blue histogram).
  • H4A3_APC_110308.jpg
    Thrombin-activated human peripheral blood platelets were stained with CD107a (clone H4A3) APC (pink histogram) or mouse IgG1, κ APC (blue histogram).
See APC spectral data
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328619 25 tests 120€
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328620 100 tests 252€
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Description

CD107a, also known as Lysosome-Associated Membrane Protein 1 (LAMP-1) or LGP-120, is a 110-140 kD type I membrane glycoprotein. Mature CD107a is heavily glycosylated from a 40 kD core protein. This molecule is located on the luminal side of lysosomes. Upon activation, CD107a is transferred to the cell membrane surface of activated platelets, activated lymphocytes, macrophages, epithelial cells, endothelial cells, and some tumor cells. CD107a has been suggested to play a role in the protection of lysosomal membrane from lysosomal hydrolases which is involved in cell adhesion and regulation of tumor metastasis, and mediates autoimmune disease progression. CD107a is a ligand for galaptin and E-selectin. Surface expression of LAMP-1 has been shown to correlate with CD8+ T cell and NK cell cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human adult adherent peripheral blood cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting8, immunohistochemical staining2, immunofluorescence5,7, and immunoprecipitation5.

This antibody is specific to human LAMP-1. Positive control: Hela cells; LAMP-1 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References
  1. Misse D, et al. 1999. Blood 93:2454.
  2. Furuta K, et al. 2001. Am. J. Pathol. 159:449. (IHC)
  3. Watanabe A, et al. 2011. J. Biol. Chem. 286:10702. PubMed
  4. Baron Gaillard CL, et al. 2011. Mol. Cell. Biol. 22:5459. PubMed
  5. Hauck CR and Meyer TF. 1997. FEBS Lett. 405:86. (IF, IP)
  6. De Keersmaecker B, et al. 2012. J. Virol. 86:9351. PubMed
  7. Knodler LA, et al. 2010. P. Natl. Acad. Sci. USA. 107:17733. (IF)
  8. Oh J, et al. 2000. Hum. Mol. Genet. 9:375. (WB)
  9. Salio M, et al. 2013 PNAS. 110:4753. PubMed
Product Citations
  1. Vinton C, et al. 2017. J Immunol. 10.4049/jimmunol.1700136. PubMed
  2. Fowler DW, et al. 2017. Cancer Immunol Immunother. 66:1205. PubMed
  3. Liu Y, et al. 2017. Oncogene. 10.1038/onc.2017.209. PubMed
  4. Agelidis A, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.06.041. PubMed
  5. Frasca D, et al. 2018. PLoS One. 13:e0197472. PubMed
  6. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  7. Wei J, et al. 2019. J Immunother Cancer. 7:209. PubMed
  8. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  9. Zhou Y, et al. 2017. Front Cell Infect Microbiol. 7:457. PubMed
  10. Iraqi M, et al. 2020. Front Cell Infect Microbiol. 10:48. PubMed
  11. Woolsey C, et al. 2020. Scientific Reports. 10(1):3071.. PubMed
  12. Gujar S, et al. 2010. Mol Cancer Ther. 2.405555556. PubMed
  13. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
  14. Davis Z, et al. 2016. PLoS Pathog. 12: 1005421. PubMed
  15. Dao TN, et al. 2020. Cancers (Basel). 12:00. PubMed
  16. Ali A, et al. 2020. J Infect Dis. 222:853. PubMed
  17. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  18. Hamdan F, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(8):. PubMed
  19. Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
  20. Harper JL, et al. 2020. Nat Med. 519:26. PubMed
  21. Elias S, et al. 2020. Haematologica. . PubMed
  22. Diab M, et al. 2020. Viruses. :12. PubMed
  23. Ding R, et al. 2021. Theranostics. 4957:11. PubMed
  24. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  25. Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed
  26. Sahillioglu AC, et al. 2021. Cancer Immunol Res. 9:999. PubMed
  27. Gruijs M, et al. 2021. Int J Mol Sci. 22:. PubMed
  28. Ma KY, et al. 2021. Nat Immunol. 22:1590. PubMed
  29. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  30. Yan LM, et al. 2020. MBio. 11:. PubMed
  31. Guo M, et al. 2021. Front Oncol. 11:632540. PubMed
  32. Guo S, et al. 2021. Stem Cells Int. 2021:9989790. PubMed
  33. Izumi K, et al. 2021. Immunohorizons. 5:687. PubMed
  34. Buijsers B, et al. 2022. Front Immunol. 12:759570. PubMed
  35. Georg P, et al. 2022. Cell. 185:493. PubMed
  36. Song C, et al. 2022. Stem Cell Res Ther. 13:48. PubMed
  37. Han F, et al. 2022. Cent Eur J Immunol. 46:427. PubMed
  38. Pathania AS, et al. 2022. Mol Ther Oncolytics. 25:308. PubMed
  39. Peluso MJ, et al. 2021. Cell Rep. 36:109518. PubMed
  40. Rambaldi B, et al. 2022. Haematologica. 107:2617. PubMed
  41. López-Sanz C, et al. 2022. STAR Protoc. 3:101755. PubMed
  42. Li Y, et al. 2022. J Oncol. 2022:4544773. PubMed
  43. Onnis A, et al. 2023. J Exp Med. 220: . PubMed
  44. Pušnik J, et al. 2023. Nat Commun. 14:572. PubMed
  45. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  46. Hamdan F, et al. 2023. Mol Ther Oncolytics. 28:264. PubMed
RRID
AB_1279057 (BioLegend Cat. No. 328619)
AB_1279055 (BioLegend Cat. No. 328620)

Antigen Details

Structure
LAMP-1 is a 417 amino acid protein with a molecular mass of 45 kD.
Distribution

Macrophages, epithelial cells, endothelial cells, some tumor cells; located on the luminal side of lysosomes or on the surface of cell membranes

Function
Protect lysosomal membrane from lysosomal hydrolases, adhesion
Ligand/Receptor
Galaptin
Cell Type
Endothelial cells, Epithelial cells, Macrophages
Biology Area
Cell Biology, Immunology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Sarafian V, et al. 2006. Arch. Dermatol. Res. 298:7381.
2. Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press.
3. Sawada R, et al. 1993. J. Biol. Chem. 268:12675.
4. Chen JW, et al. 1988. J. Biol. Chem. 263:8754.
5. Chen JW, et al. 1986. Biochem. Soc. Symp. 51:97112.

Gene ID
3916 View all products for this Gene ID
UniProt
View information about CD107a on UniProt.org
Go To Top Version: 2    Revision Date: 10.22.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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