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APC anti-human TCR α/β Antibody

Pricing & Availability
Clone
IP26 (See other available formats)
Regulatory Status
RUO
Other Names
α/β TCR, TCR α/β
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
IP26_APC_062410
Human peripheral blood lymphocytes stained with IP26 APC
  • IP26_APC_062410
    Human peripheral blood lymphocytes stained with IP26 APC
See APC spectral data
Cat # Size Price Quantity Check Availability Save
306717 25 tests 91€
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306718 100 tests 200€
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Description

The IP26 antibody reacts with a monomorphic determinant of the α/β T-cell receptor, which is expressed on greater than 95% of normal peripheral blood CD3+ T cells. The α/β TCR recognizes a peptide bound to MHC leading to T-cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: T cell activation. When co-staining with anti-CD3, we recommend using clone UCHT1, since we have confirmed that IP26 does not compete with this clone.  Other anti-CD3 clones may compete out the binding of IP26.  

Application References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York. (FC)
  2. Joseph A, et al. 2008. J. Virol. 82:3078. (FC) PubMed
  3. Pinto JP, et al. 2010. Immunology. 130:217. PubMed
Product Citations
  1. Frasca D, et al. 2018. PLoS One. 13:e0197472. PubMed
  2. Omer B, et al. 2019. Front Med (Lausanne). 5:343. PubMed
  3. Jiménez–Reinoso A, et al. 2018. J Allergy Clin Immunol. 141:1151. PubMed
  4. Ollé Hurtado M, et al. 2019. PLoS One. 14:e0216373. PubMed
  5. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  6. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  7. Rosskopf S, et al. 2018. Oncotarget. 9:17608. PubMed
  8. Guo X, et al. 2016. Mol Ther Methods Clin Dev. 3:15054. PubMed
  9. Fisher J, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.03.002. PubMed
  10. Congras A, et al. 2020. J Clin Invest. 130:6395. PubMed
  11. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  12. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  13. Japp AS, et al. 2021. Cell. 184(3):827-839.e14. PubMed
  14. Bear AS, et al. 2021. Nat Commun. 12:4365. PubMed
  15. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  16. Wang Z, et al. 2021. Cell Mol Immunol. 18:2188. PubMed
  17. Ferry GM, et al. 2022. Front Immunol. 13:863155. PubMed
  18. Warmuth S, et al. 2022. Oncoimmunology. 10:2004661. PubMed
  19. Durgin JS, et al. 2022. Mol Ther. 30:1201. PubMed
  20. Anadon CM, et al. 2022. Cancer Cell. 40:545. PubMed
  21. Zhang Q, et al. 2022. Bio Protoc. 12:. PubMed
  22. Lam D, et al. 2023. Nat Biotechnol. 41:686. PubMed
  23. Lah S, et al. 2023. J Immunother Cancer. 11:. PubMed
RRID
AB_10612747 (BioLegend Cat. No. 306717)
AB_10612569 (BioLegend Cat. No. 306718)

Antigen Details

Structure
Ig superfamily, with CD3 forms CD3/TCR complex
Distribution

T cells, thymocytes

Function
Antigen recognition, T cell activation
Ligand/Receptor
Peptide bound to MHC
Cell Type
T cells, Thymocytes, Tregs
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Marchalonis J, et al. 2002. J. Mol. Recognit. 15:260.

Gene ID
6955 View all products for this Gene ID 6957 View all products for this Gene ID
UniProt
View information about TCR alpha/beta on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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