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APC anti-human TNF-α Antibody

Pricing & Availability
Clone
MAb11 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_Mab11_APC_070609.jpg
PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes stained with mouse IgG1 APC isotype control and CD3 (UCHT1) PE
  • A_Mab11_APC_070609.jpg
    PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes stained with mouse IgG1 APC isotype control and CD3 (UCHT1) PE
  • B_Mab11_APC_070609.jpg
    PMA/ionomycin-stimulated (6 hours) human peripheral blood lymphocytes stained with MAb11 APC and CD3 (UCHT1) PE
See APC spectral data
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502913 50 µg 129€
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502912 100 tests 224€
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli-expressed, recombinant human TNF-α
Formulation
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

ELISA or ELISPOT Detection: The biotinylated MAb11 antibody is useful as the detection antibody in a sandwich ELISA or ELISPOT, when used in conjunction with the purified MAb1 antibody (Cat. No. 502802/502804) as the capture antibody.

Flow Cytometry3,5,6,10: The fluorochrome-labeled MAb11 antibody is useful for intracellular and membrane-bound immunofluorescent staining and flow cytometric analysis to identify TNF-a-producing cells within mixed cell populations.

Additional reported applications (for the relevant formats) include: neutralization1,2, immunohistochemical staining of paraformaldehyde-fixed, saponin-treated frozen tissue sections4 and acetone-fixed frozen tissue sections8, immunocytochemistry7, and immunofluorescence9. The MAb11 antibody can neutralize the bioactivity of natural or recombinant TNF-a.

Note: For testing human TNF-a in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430201 to 430206) are specially developed and recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human TNF-a bioactivity (Cat. No. 502922).

The Purified MAb1 antibody is useful in neutralization2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody.

Clone MAb11 cross-reacts to Cat11

Application References
  1. Rathjen D, et al. 1991. Mol. Immunol. 28:79. (Neut)
  2. Ablamunits V, et al. 2010. Eur. J. Immunol. 40:2891. (Neut)
  3. Enr quez J, et al. 2002. Adv. Perit. Dial. 18:177. (ICFC)
  4. Andersson U, et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag. (IHC)
  5. Chen H, et al. 2005. J. Immunol. 175:591. (ICFC)
  6. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  7. Andersson U, et al. 2000. J. Exp. Med. 192:565. (ICC)
  8. Moormann AM, et al. 1999. J. Infect. Dis. 180:1987. (IHC)
  9. Zhao XJ, et al. 2003. J. Immunol. 170:2923. (IF)
  10. Rieger R, et al. 2009. Cancer Gene Ther. 1:53-64. (FC)
  11. Maksaereekul S, et al. 2009. Vaccine. 28:3754 (FC)
Product Citations
  1. Liu Z, et al. 2018. Sci Rep. 8:703. PubMed
  2. Sun Z, et al. 2019. J Cell Mol Med. 23:887. PubMed
  3. Yu J, et al. 2019. Oncol Lett. 17:1461. PubMed
  4. Abd Hamid M et al. 2019. Cancer Immunol Res. 7(8):1293-1306 . PubMed
  5. Vacaflores A, et al. 2017. Mol Immunol. 81:1. PubMed
  6. Vitallé J, et al. 2017. Front Immunol. 8:836. PubMed
  7. Abd Hamid M, et al. 2020. Frontiers in Immunology. 0.673611111. PubMed
  8. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  9. Steindor M, et al. 2015. PLoS One. 10:119737. PubMed
  10. Schmidt E, et al. 2015. Brain Behav Immun. 47: 178-185. PubMed
  11. Bsat M, et al. 2015. J Leukoc Biol. 98: 671 - 681. PubMed
  12. Cuff AO, Robertson FP 2016. J Immunol. 197(11):4283-4291. PubMed
  13. Orrantia A, et al. 2020. STAR Protoc. 1:100149. PubMed
  14. Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed
  15. Sánchez NC, et al. 2020. DNA Cell Biol. 39:484. PubMed
  16. Cai J, et al. 2021. eLife. 10:00. PubMed
  17. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  18. Bonifacius A, et al. 2021. Immunity. 54(2):340-354.e6. PubMed
  19. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  20. Rappazzo CG, et al. 2020. bioRxiv. . PubMed
  21. Bartolomé-Casado R, et al. 2020. Mucosal Immunol. 14:402. PubMed
  22. Volpin V, et al. 2020. Cancer Immunol Res. 8:1163. PubMed
  23. Oda SK, et al. 2020. J Exp Med. 217: . PubMed
  24. Peng W, et al. 2020. Antimicrob Agents Chemother. 64:. PubMed
  25. Platten M, et al. 2021. Nature. 592:463. PubMed
  26. Shao L, et al. 2021. Cell Death Discov. 7:145. PubMed
  27. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  28. Prakash MD, et al. 2021. PLoS One. 16:e0247492. PubMed
  29. Gussarow D, et al. 2021. Front Med (Lausanne). 8:770381. PubMed
  30. Ridley ML, et al. 2020. J Immunol. 204:2940. PubMed
  31. Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed
  32. Olafsdottir TA, et al. 2022. Commun Biol. 5:914. PubMed
  33. Orrantia A, et al. 2022. iScience. 25:105235. PubMed
  34. Bates TA, et al. 2022. Cell Rep. 41:111544. PubMed
  35. Pymm P, et al. 2022. Cell Rep. 38:110449. PubMed
  36. Du W, et al. 2022. FEBS Open Bio. 12:1771. PubMed
  37. Inaba A, et al. 2023. Nat Commun. 14:2071. PubMed
RRID
AB_315265 (BioLegend Cat. No. 502913)
AB_315264 (BioLegend Cat. No. 502912)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 17 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Neutrophils, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.

Regulation
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists
Gene ID
7124 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org
Go To Top Version: 3    Revision Date: 09.06.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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