- Clone
- 1D3/CD19 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- B4, CVID3
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![1D3slashCD19_APC_CD19_Antibody_092616 1D3slashCD19_APC_CD19_Antibody_092616](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/1D3slashCD19_APC_CD19_Antibody_092616.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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C57BL/6 mouse splenocytes were stained with CD19 APC (clone 1D3/CD19, filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
152409 | 25 µg | 76€ | ||||
152410 | 100 µg | 224€ |
CD19 is a 95 kD glycoprotein also known as B4. It is a member of the Ig superfamily, expressed on all pro-B to mature B cells (during development) and follicular dendritic cells. Plasma cells do not express CD19. CD19, in association with CD21 and CD81, forms a molecular complex integral to B cell activation.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Transfected k562 cells expressing the extracellular domain of murine CD19.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: blocking1,2 and immunoprecipitation1.
- Application References
-
- Krop I, et al. 1996. Eur. J. Immunol. 26:238. (FC, Blocking, IP)
- Abraham P, et al. 2014. Clin. Exp. Immunol. 175:181. (Blocking)
- Product Citations
- RRID
-
AB_2629838 (BioLegend Cat. No. 152409)
AB_2629839 (BioLegend Cat. No. 152410)
Antigen Details
- Structure
- Ig superfamily, associates with CD21 and CD81, 95 kD.
- Distribution
-
Pro-B cells to mature B cells (during development), follicular dendritic cells.
- Function
- Modulates B cell activation and differentiation.
- Ligand/Receptor
- CD21, CD81, Leu-13.
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Fearon DT. 1993. Curr. Opin. Immunol. 5:341.
2. Krop I, et al. 1996. Eur. J. Immunol. 26:238.
3. Krop I, et al. 1996. J. Immunol. 157:48.
4. Tedder TF, et al. 1994. Immunol. Today 15:437. - Gene ID
- 12478 View all products for this Gene ID
- UniProt
- View information about CD19 on UniProt.org
Related FAQs
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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