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APC/Cyanine7 anti-human CD3 Antibody

Pricing & Availability
Clone
SK7 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK7_APCCyanine7_CD3_Antibody_011819.png
Human peripheral blood lymphocytes were stained with CD3 (clone SK7) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
  • SK7_APCCyanine7_CD3_Antibody_011819.png
    Human peripheral blood lymphocytes were stained with CD3 (clone SK7) APC/Cyanine7 (filled histogram) or mouse IgG1, κ APC/Cyanine7 isotype control (open histogram)
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
344817 25 tests 104€
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344818 100 tests 234€
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
  1. Kan EA, et al. 1983. J. Immunol. 131:536.
  2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
  3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
  5. Carbone A, et al. 1999. Blood 93:2319. (IHC)
  6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
  7. Rutjens E, et al. 2007. J. Immunol. 178:1702.
  8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  2. Christiansen M, et al. 2020. Front Immunol. 10:3022. PubMed
  3. Salazar-Camarena DC, et al. 2020. Sci Rep. 10:6236. PubMed
  4. Mothe B, et al. 2020. Front Immunol. 1.029861111. PubMed
  5. Yamaguchi K, et al. 2018. Cancer Sci. 109:3032. PubMed
  6. Chao Y, et al. 2014. J Leukoc Biol. 96:1037. PubMed
  7. Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed
  8. Tang X, et al. 2020. Sci Adv. 6:eaaz0374. PubMed
  9. Xie G, et al. 2021. Cell Reports. 35(4):109038. PubMed
  10. Graham C, et al. 2021. Immunity. 54(6):1276-1289.e6. PubMed
  11. Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
  12. Calabrese DR, et al. 2020. J Clin Invest. . PubMed
  13. Thompson EA, et al. 2021. Cell Rep. 108863:34. PubMed
  14. Egedal JH, et al. 2021. Mucosal Immunol. 14:1203. PubMed
  15. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  16. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  17. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  18. Muliaditan T, et al. 2021. Cell Rep Med. 2:100457. PubMed
  19. Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
  20. Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed
  21. Guerrero A, et al. 2022. Nat Aging. 2:851. PubMed
  22. Yuan H, et al. 2022. J Clin Exp Hematop. 62:52. PubMed
  23. Cox MJ, et al. 2022. Leukemia. 36:1635. PubMed
  24. Idiz UO, et al. 2022. J Med Virol. 94:4744. PubMed
  25. Ma T, et al. 2022. Elife. 11: . PubMed
  26. Sungalee S, et al. 2021. Nat Genet. 53:650. PubMed
  27. Hastie KM, et al. 2023. Cell Rep. 42:112421. PubMed
RRID
AB_10644011 (BioLegend Cat. No. 344817)
AB_10645474 (BioLegend Cat. No. 344818)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, during thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 2    Revision Date: 01.18.2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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