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APC/Cyanine7 anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
  • TER-119_APCCyanine7_TER119-Erythroid_Antibody_020119
    C57BL/6 bone marrow cells were stained with TER-119 APC/Cyanine7 (filled histogram) or Rat IgG2b, κ APC/Cyanine7 isotype control (open histogram). Data shown was gated on small scatter population.
See APC/Cyanine7 spectral data
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116223 25 µg 76€
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References
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  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
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  25. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  26. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
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  34. Venugopal J, et al. 2020. Sci Rep. 10:16514. PubMed
  35. Zhang C, et al. 2020. Cell Rep. 32:108206. PubMed
  36. Cheung P, et al. 2020. Cell Stem Cell. 27(4):590-604.e9. PubMed
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  58. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
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RRID
AB_2137788 (BioLegend Cat. No. 116223)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org
Go To Top Version: 3    Revision Date: 02.01.2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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