- Clone
- RPA-2.10 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV T085
- Other Names
- LFA-2, T11, SRBC-R
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![RPA-2dot10_APCFIRE750_CD2_Antibody_121018 RPA-2dot10_APCFIRE750_CD2_Antibody_121018](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/RPA-2dot10_APCFIRE750_CD2_Antibody_121018.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood lymphocytes stained with CD2 (clone RPA-2.10) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
300225 | 25 tests | 123€ | ||||
300226 | 100 tests | 244€ |
CD2 is a 50 kD type I transmembrane glycoprotein also known as LFA-2, T11, and sheep red blood cell receptor (SRBC-R). This immunoglobulin superfamily member is expressed on thymocytes, T lymphocytes, NK cells, and thymic B cell subsets. The major ligand for CD2 is CD58 (also known as LFA-3). CD2 has also been reported to bind CD48, CD59, and CD15. CD2 plays a critical role in alternative T cell activation, T cell signaling, and cell-cell adhesion.
Product DetailsProduct Details
- Reactivity
- Human,Cynomolgus,Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm. - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections6 and blocking of T cell activation2. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 300239 & 300240).
- Application References
-
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Aversa G, et al. 1987. Transplant. Proc. 19:277. (Block)
- Zaretsky AG, et al. 2009. J. Exp Med. 206:991. (IHC) PubMed
- Perona-Wright G, et al. 2010. Nat. Immunol. 11:520. (FC) PubMed
- Thummler K, et al. 2010. J. Leukoc. Biol. 88:1041.
- Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- RRID
-
AB_2783147 (BioLegend Cat. No. 300225)
AB_2783148 (BioLegend Cat. No. 300226)
Antigen Details
- Structure
- Ig superfamily, type I transmembrane glycoprotein, 50 kD
- Distribution
-
T cells, NK cells, thymocytes, and thymic B cell subsets
- Function
- T cell activation, adhesion
- Ligand/Receptor
- CD58 (LFA-3), CD48, CD59, CD15
- Cell Type
- B cells, NK cells, T cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
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1. Bell G, et al. 1995. J. Immunol. 155:2805.
2. Bierer B, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Moingeon P, et al. 1989. Immunol. Rev. 111:111. - Gene ID
- 914 View all products for this Gene ID
- UniProt
- View information about CD2 on UniProt.org
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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