- Clone
- 4G8 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
- Previously
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Signet Catalog# 9240-02
Signet Catalog# 9240-05
Signet Catalog# 9240-10
Covance Catalog# SIG-39240
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![4G8_Biotin_Beta-Amyloid17-24_Antibody_1_090718 4G8_Biotin_Beta-Amyloid17-24_Antibody_1_090718](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/4G8_Biotin_Beta-Amyloid17-24_Antibody_1_090718.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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IHC staining of Biotin anti-β-Amyloid, 17-24 antibody (clone 4G8) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit were used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of Biotin anti-β-Amyloid, 17-24 antibody (clone 4G8) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 1 µg/ml of the primary antibody for 60 minutes at room temperature. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit were used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
Direct ELISA of Biotin anti-β-Amyloid, 17-24 (clone 4G8) antibody binding to the plate-immobilized human Aβ1-40, human Aβ1-42, and recombinant human APP751 protein. ELISA was performed by coating the wells with 100 ng of peptide or recombinant protein. The wells were then incubated with the primary antibody at 37°C for 45 minutes followed by incubation with HRP labeled streptavidin. TMB (3, 3', 5, 5' tetramethylbenzidine, Cat. No. 421501) was used as the detection system.
Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.
Product DetailsProduct Details
- Reactivity
- Human,Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution (no preservatives or carrier proteins).
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC-P - Quality tested
Direct ELISA - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 2.0 µg/ml is suggested. For Direct ELISA applications, a concentration range of 0.5 - 2.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This antibody is reactive to amino acid residues 17-24 of ß amyloid. The epitope lies within amino acids 18-22 of ß amyloid (VFFAE). 4G8 ß-amyloid antibody reacts to abnormally processed isoforms, as well as precursor forms.
This antibody clone has been reported for use on IHC of free-floating sections in PBS containing 1% Triton incubated with 0.1 m citrate buffer (4).
Additional reported applications (for the relevant formats) include: immunohistochemical staining on frozen tissue sections (IHC-F) and immunocytochemistry (ICC) - Additional Product Notes
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View more applications data for this product in our Scientific Poster Library.
- Application References
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- Poduslo JF, et al. 2004. Biochem. 43:6064. (IHC-F) PubMed
- Forny-Germano L, et al. 2014. J Neurosci. 34:13629. (IHC-Other) PubMed
- Vallino Costassa E, et al. 2016. J Alzheimers Dis. 51: 875-87. (IHC-P) PubMed
- Chen X, et al. 2013. Neurobiol Aging. 34:2370. (ICC) PubMed
- Hatami A, et al. 2016. J Alzheimers Dis. 50:517. (IHC-P) PubMed
- Kawarabayashi T, et al. 2001. J Neurosci 21:372 (IP)
- Fonte V, et al. 2002. PNAS. 110:4853 (IP)
- Product Citations
- RRID
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AB_2564649 (BioLegend Cat. No. 800706)
AB_2564651 (BioLegend Cat. No. 800705)
AB_2564650 (BioLegend Cat. No. 800704)
Antigen Details
- Structure
- Amyloid precursor protein is a 770 amino acid protein with a molecular mass of ~100 kD. According to the UniProtKB database, APP (ID# P05067) has 11 isoforms (34 to ~90 kD) and the 770 form has been designated as the canonical form. Isoform APP695 is the predominant form expressed in neuronal tissue. Isoforms APP751 and APP770 are widely expressed in non-neuronal cells. Isoform APP751 is the most abundant form in T-lymphocytes. Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
- Distribution
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Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus. - Function
- The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid
- Antigen References
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- Kumar A, et al. 2015. Pharmacol Rep. 67(2):195.
- Sadigh-Eteghad S, et al. 2015. Med Princ Pract. 24(1):1
- Hampel H, et al. 2015. Expert Rev Neruother. 15(1):83.
- Puig KL, et al. 2012. Exp Gerontol. 48(7): 608.
- Selkoe DJ, et al. 2016. EMBO Mol Med. 8(6):595.
- Walsh DM, et al. 2007. J Neurochem. 101(5):1172.
- Gene ID
- 351 View all products for this Gene ID
- UniProt
- View information about beta-Amyloid 17-24 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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