Loading...

Brilliant Violet 510™ anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
publications
M170_BV510_CD11b_Antibody_FC_082512
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 510™. Data shown was gated on the myeloid cell population.
  • M170_BV510_CD11b_Antibody_FC_082512
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Brilliant Violet 510™. Data shown was gated on the myeloid cell population.
  • M170_BV510_CD11b_Antibody_ICC_121319
    C57 mouse bone marrow cells were fixed with 2% paraformaldehyde (PFA), and then stained with 10 µg/ml of CD11b (clone M1/70) Brilliant Violet 510™ (red) and 10 µg/ml of CD45 (clone 30-F11) Alexa Fluor® 647 (blue) for 30 minutes at room temperature. The image was captured by 60X objective.
See Brilliant Violet 510™ spectral data See high resolution IF data...
Cat # Size Price Quantity Check Availability Save
101245 125 µL 132€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
101263 50 µg 206€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse,Human,Cynomolgus,Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µL size, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 0.4 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, immunohistochemistry of acetone-fixed frozen sections5,11-13, and spatial biology (IBEX)35,36. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  35. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  36. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  2. LaMothe RA et al. 2018. Front Immunol. 0.570138889 . PubMed
  3. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  4. Le TM et al. 2018. Journal of neurochemistry. 145(2):139-153 . PubMed
  5. Luo J et al. 2018. Immunity. 49(1):107-119 . PubMed
  6. Gentek R, et al. 2018. Immunity. 48:1160. PubMed
  7. Nakamoto N, et al. 2019. Nat Microbiol. 4:492. PubMed
  8. Uderhardt S, et al. 2019. Cell. 177:541. PubMed
  9. Eremenko E, et al. 2019. EBioMedicine. 43:424. PubMed
  10. Ramakrishna C, et al. 2019. Nat Commun. 10:2153. PubMed
  11. Lyons J, et al. 2018. Sci Signal. 11. PubMed
  12. Christ A, et al. 2018. Cell. 172:162. PubMed
  13. Smith CA, et al. 2019. JCI Insight. 4. PubMed
  14. Meixiong J et al. 2019. Immunity. 50(5):1163-1171 . PubMed
  15. Green DP, et al. 2019. Neuron. 101:412. PubMed
  16. McDonald LT, et al. 2017. PLoS One. 12:e0180724. PubMed
  17. Lee C, et al. 2020. Front Immunol. 11:77. PubMed
  18. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  19. Bhattacherjee A, et al. 2019. Commun Biol. 2:450. PubMed
  20. Schaffert A, et al. 2020. Front Immunol. 10:3024. PubMed
  21. Hoffmann FM, et al. 2018. Front Immunol. 9:1046. PubMed
  22. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  23. Briukhovetska D, et al. 2020. Frontiers in Immunology. 1.428472222. PubMed
  24. McCauley ME, et al. 2020. Nature. 585(7823):96-101. PubMed
  25. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  26. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  27. Karsten C, et al. 2015. J Immunol. 194:1841. PubMed
  28. Cardoso M, et al. 2015. Infect Immun . 83: 3534-3544. PubMed
  29. Zhang Y, et al. 2015. PLoS Pathog. 11: e1005167. PubMed
  30. Woods T, et al. 2016. J Virol. 90: 2783 - 2793. PubMed
  31. Peterson T, et al. 2016. Proc Natl Acad Sci U S A. 113: 4470-4475. PubMed
  32. Kloepper J, et al. 2016. Proc Natl Acad Sci U S A. 113: 4476-4481. PubMed
  33. Kostadinova E, et al. 2016. Sci Rep. 6:30943. PubMed
  34. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  35. Vicetti Miguel R, et al. 2016. PLoS One. 11: 0162445. PubMed
  36. Ender F, et al. 2017. PLoS One. 12(2):e0172446. PubMed
  37. Wang Y, et al. 2020. Cell Death Dis. 1.169444444. PubMed
  38. El Amki M, et al. 2020. Cell Rep. 33:108260. PubMed
  39. Kerkhofs D, et al. 2020. Theranostics. 7.022222222. PubMed
  40. Burns JC, et al. 2020. eLife. 9:00. PubMed
  41. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  42. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  43. Plumlee CR, et al. 2020. Cell Host Microbe. 29(1):68-82.e5. PubMed
  44. Ringel AE, et al. 2020. Cell. 183(7):1848-1866.e26. PubMed
  45. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  46. Affo S, et al. 2021. Cancer Cell. 39(6):866-882.e11. PubMed
  47. Platt DJ, et al. 2021. Cell Reports. 35(6):109113. PubMed
  48. Shmuel-Galia L, et al. 2021. Immunity. 54(6):1137-1153.e8. PubMed
  49. Ahn D, et al. 2021. Cell Reports. 35(9):109196. PubMed
  50. Wei Y, et al. 2021. Endocrinology. 162(8):. PubMed
  51. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  52. Zheng J, et al. 2020. bioRxiv. . PubMed
  53. Zheng J, et al. 2020. Nature. . PubMed
  54. Laumonnier Y, et al. 2020. Curr Protoc Immunol. 130:e100. PubMed
  55. Wang X, et al. 2021. Theranostics. 11:209. PubMed
  56. Ma Q, et al. 2021. Clin Transl Immunology. 10:e1300. PubMed
  57. Kim YS, et al. 2021. Cell Death Dis. 12:307. PubMed
  58. Platten M, et al. 2021. Nature. 592:463. PubMed
  59. Gangoso E, et al. 2021. Cell. 184:2454. PubMed
  60. Roberts LM, et al. 2021. iScience. 24:103025. PubMed
  61. Maulloo CD, et al. 2021. Front Immunol. 12:714842. PubMed
  62. Wong J, et al. 2020. PLoS One. 15:e0233794. PubMed
  63. Mincham KT, et al. 2021. Front Immunol. 11:601494. PubMed
  64. Jeljeli M, et al. 2021. Front Immunol. 12:670776. PubMed
  65. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  66. Bresque M, et al. 2022. J Biol Chem. :101711. PubMed
  67. Yuan M, et al. 2022. Oxid Med Cell Longev. 2022:5479491. PubMed
  68. Vogel A, et al. 2022. Cell Rep. 38:110420. PubMed
  69. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  70. Mitsune A, et al. 2021. Respir Res. 22:232. PubMed
  71. Chen X, et al. 2021. Cell Rep. 37:109991. PubMed
  72. Kim S, et al. 2022. Redox Biol. 54:102347. PubMed
  73. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  74. Puigdelloses M, et al. 2021. J Immunother Cancer. 9:. PubMed
  75. Burns JC, et al. 2021. Bio Protoc. 11:e4091. PubMed
  76. Samuelson DR, et al. 2021. Commun Biol. 4:997. PubMed
  77. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  78. Celorrio M, et al. 2022. Acta Neuropathol Commun. 10:10. PubMed
  79. Ma C, et al. 2022. Proc Natl Acad Sci U S A. 119:. PubMed
  80. Akagi T, et al. 2022. Front Immunol. 13:926175. PubMed
  81. Park JH, et al. 2022. STAR Protoc. 3:101607. PubMed
  82. Papafragkos I, et al. 2022. Front Immunol. 13:889075. PubMed
  83. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  84. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  85. Xu G, et al. 2022. Front Pharmacol. 13:896601. PubMed
  86. Zheng X, et al. 2020. Int J Cancer. 146:1730. PubMed
  87. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  88. Ma Y, et al. 2022. Front Immunol. 13:816952. PubMed
  89. Chryplewicz A, et al. 2022. Cancer Cell. 40:1111. PubMed
  90. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  91. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  92. Li H, et al. 2022. Cancer Cell. 40:36. PubMed
  93. Fang C, et al. 2022. J Pathol. 256:310. PubMed
  94. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  95. Tu J, et al. 2022. Cancer Sci. 113:2496. PubMed
  96. Dolfi B, et al. 2022. Cell Rep. 39:110949. PubMed
  97. Campisi L, et al. 2022. Nature. 606:945. PubMed
  98. Jiang H, et al. 2022. Oxid Med Cell Longev. 2022:5064494. PubMed
  99. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  100. Xu H, et al. 2022. Aging Dis. 13:1875. PubMed
  101. Montilla A, et al. 2023. Cell Death Dis. 14:16. PubMed
  102. Gonçalves R, et al. 2023. iScience. 26:105972. PubMed
  103. Kemna J, et al. 2023. Nat Immunol. 24:414. PubMed
  104. Jameson VJ, et al. 2022. Cytometry A. 101:922. PubMed
  105. Pérez-Torrado V, et al. 2022. Bio Protoc. 12:. PubMed
  106. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  107. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  108. Daum P, et al. 2023. Front Immunol. 13:991347. PubMed
  109. Solier S, et al. 2023. Int J Mol Sci. 24:. PubMed
  110. Sheng Y, et al. 2023. Cancers (Basel). 15:. PubMed
  111. Xu H, et al. 2023. Elife. 12:. PubMed
  112. Read KA, et al. 2023. Nat Commun. 14:1652. PubMed
  113. Zhang Y, et al. 2023. J Immunol Res. 2023:5867047. PubMed
  114. Feng X, et al. 2023. Nat Commun. 14:3208. PubMed
RRID
AB_2561390 (BioLegend Cat. No. 101245)
AB_2629529 (BioLegend Cat. No. 101263)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org
Go To Top Version: 3    Revision Date: 07.08.2016

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account