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Brilliant Violet 650™ anti-human CD69 Antibody

Pricing & Availability
Clone
FN50 (See other available formats)
Regulatory Status
RUO
Workshop
IV A91
Other Names
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
FN50_BV650_CD69_Antibody_FC_011713.jpg
PMA + ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were stained with CD69 (clone FN50) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).
  • FN50_BV650_CD69_Antibody_FC_011713.jpg
    PMA + ionomycin-stimulated (6 hours) human peripheral blood lymphocytes were stained with CD69 (clone FN50) Brilliant Violet 650™ (filled histogram) or mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).
See Brilliant Violet 650™ spectral data
Cat # Size Price Quantity Check Availability Save
310933 25 tests 176€
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310934 100 tests 317€
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Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.

Application References
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Fu J et al. 2019. Cell stem cell. 24(2):227-239 . PubMed
  2. Siddiqui I et al. 2019. Immunity. 50(1):195-211 . PubMed
  3. Karlsson J, et al. 2020. Nat Commun. 1.773611111. PubMed
  4. Sam J, et al. 2020. Front Oncol. 10:575737. PubMed
  5. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  6. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  7. Tauzin A, et al. 2021. Cell Host Microbe. . PubMed
  8. Nel I, et al. 2021. Diabetologia. 64(10):2306-2321. PubMed
  9. Fu J, et al. 2021. J Clin Invest. 131:. PubMed
  10. Bovay A, et al. 2020. Hum Vaccin Immunother. 16:3103. PubMed
  11. Gao Y, et al. 2022. Immunity. 55:1732. PubMed
  12. Sudmeier LJ, et al. 2022. Cell Rep Med. 3:100620. PubMed
  13. Nayrac M, et al. 2022. Cell Rep. 39:111013. PubMed
  14. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  15. Ezeonwumelu IJ, et al. 2022. Int J Mol Sci. 23: . PubMed
  16. Xu Q, et al. 2023. Nat Immunol. 24:186. PubMed
  17. Hsiao CC, et al. 2023. iScience. 26:105785. PubMed
  18. Nicolas A, et al. 2023. iScience. 26:105904. PubMed
  19. Kerns JS, et al. 2023. Bio Protoc. 13: . PubMed
  20. Collora JA, et al. 2022. Immunity. 55:1013. PubMed
  21. De Biasi S, et al. 2023. Curr Protoc. 3:e636. PubMed
  22. Sannier G, et al. 2023. Cell Rep Med. 4:100955. PubMed
  23. Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed
RRID
AB_2561783 (BioLegend Cat. No. 310933)
AB_2563158 (BioLegend Cat. No. 310934)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02.04.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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