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FITC anti-human CD14 Antibody

Pricing & Availability
Clone
63D3 (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
63D3_FITC_CD14_Antibody_021216
Human peripheral blood monocytes were stained with FITC anti-human CD14 (clone 63D3) (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
  • 63D3_FITC_CD14_Antibody_021216
    Human peripheral blood monocytes were stained with FITC anti-human CD14 (clone 63D3) (filled histogram) or mouse IgG1, κ FITC isotype control (open histogram).
See FITC spectral data
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367115 25 tests 72€
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367116 100 tests 165€
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human peripheral blood monocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application References
  1. Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028.
  2. Devitt A, et al. 1998. Nature 6675:505.
Product Citations
  1. Motedayyen H, et al. 2017. BMC Res Notes. . 10.1186/s13104-017-2880-6. PubMed
  2. Su S et al. 2018. Cell. 175(2):442-457 . PubMed
  3. Sullivan KD et al. 2016. eLife. 5 pii: e16220. PubMed
  4. Taura M, et al. 2019. Proc Natl Acad Sci U S A. 116:2282. PubMed
  5. He Z, et al. 2020. J Exp Clin Cancer Res. 39:140. PubMed
  6. Lauterbach MA, et al. 2020. Immunity. 51(6):997-1011.e7.. PubMed
  7. Hu X, et al. 2020. Neoplasia. 1.290972222. PubMed
  8. He XP, et al. 2018. Mol Med Rep. 3.6125. PubMed
  9. Kang HM, et al. 2018. Nat Biotechnol. 36:89. PubMed
  10. Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed
  11. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  12. Yan W, et al. 2020. Biology of Reproduction. . PubMed
  13. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  14. Qing P, et al. 2020. Exp Physiol. 1708:105. PubMed
  15. Gorby C, et al. 2020. Sci Signal. :13. PubMed
  16. Lv J, et al. 2021. Mol Med Rep. 1. PubMed
  17. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  18. Besnier M, et al. 2021. Antioxidants (Basel). 10:. PubMed
  19. Fitzgerald KC, et al. 2021. Cell Rep Med. 2:100424. PubMed
  20. Li J, et al. 2021. Front Immunol. 12:649285. PubMed
  21. Wendisch D, et al. 2021. Cell. 184:6243. PubMed
  22. Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed
  23. Singh VK, et al. 2022. Front Immunol. 13:865503. PubMed
  24. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  25. Jiao Q, et al. 2020. Br J Dermatol. 182:648. PubMed
  26. Yusenko MV, et al. 2021. Cancers (Basel). 14: . PubMed
  27. Gomez-Salinero JM, et al. 2022. Cell Stem Cell. 29:593. PubMed
  28. Baudet A, et al. 2022. Bio Protoc. 12:e4353. PubMed
  29. Ji K, et al. 2023. Clin Transl Med. 13:e1234. PubMed
RRID
AB_2571928 (BioLegend Cat. No. 367115)
AB_2571929 (BioLegend Cat. No. 367116)

Antigen Details

Structure
GPI-linked membrane glycoprotein.
Distribution

Monocytes, macrophages, dendritic cells, and granulocytes.

Function
LPS receptor, clearance of Gram-negative pathogens.
Interaction
LPS.
Ligand/Receptor
LPS.
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem. J. 268:275.
  2. Wright SD, et al. 1990. Science 4975:1431.
Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org
Go To Top Version: 2    Revision Date: 11.14.2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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