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Pacific Blue™ anti-mouse CD11c Antibody

Pricing & Availability
Clone
N418 (See other available formats)
Regulatory Status
RUO
Other Names
αX integrin, integrin αX chain, CR4, p150, ITGAX
Isotype
Armenian Hamster IgG
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Product Citations
publications
N418_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ N418
  • N418_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ N418
See Pacific Blue™ spectral data
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117321 25 µg 76€
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117322 100 µg 172€
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Description

CD11c is a 150 kD glycoprotein also known as αX integrin, CR4, and p150. CD11c forms a αXβ2 heterodimer with β2 integrin (CD18). It is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells. The αXβ2 integrin plays an important role in cell-cell contact by binding its ligands: iC3b, fibrinogen, and CD54.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse spleen dendritic cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD11c antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation3, immunohistochemical staining of acetone-fixed frozen sections3, immunofluorescence microscopy5, 9 (Alexa Fluor® 488 conjugated N418 was used for IHC in frozen sections10), and spatial biology (IBEX)22,23.

Application References
  1. Granucci F, et al. 1997. J. Immunol. 159:1794.
  2. Stokes RW, et al. 1998. J. Immunol. 160:5514.
  3. Metlay JP, et al. 1990. J. Exp. Med. 171:1753. (IHC, IP)
  4. Ma XT, et al. 2006. Cancer Research 66:1169.
  5. Chin RK, et al. 2006. J. Immunol. 177:290. (IF)
  6. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC) PubMed
  7. Turnquist HR, et al. 2007. J. Immunol. 178:7018. (FC) PubMed
  8. Benson MJ, et al. 2007. J. Exp. Med. doi:10.1084/jem.20070719. (FC) PubMed
  9. You Y, et al. 2009. J. Immunol. 182:7343. (IF) PubMed
  10. Roland CL, et al. 2009. Mol. Cancer Res. 8:1761. (IHC, FC) PubMed
  11. Wikstrom M, et al.2006. J. Immunol. 177:913. PubMed
  12. Pericolini E, et al. 2008. J. Leukocyte Biol. 83:1286. PubMed
  13. Randall LM, et al. 2008. Infect. Immun.76:3312. PubMed
  14. Fahlen-Yrild L, et al. 2009. J. Immunol. 183:5032. PubMed
  15. Osterholzer JJ, et al. 2009. J. Immunol. 183:8044. PubMed
  16. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  17. Eisenach PA, et al. 2010. J Cell Sci. 123:4182. PubMed
  18. Leppin K, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:3603. PubMed
  19. Sakai F, et al. 2014. PLoS One. 9:105370. PubMed
  20. Gibbins JD, et al. 2014. Blood. 124:2953. PubMed
  21. White CE, et al. 2015. J Immunol. 194:697. PubMed
  22. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gonçalves NP, et al. 2017. J Neuroinflammation. 14:115. PubMed
  2. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  3. Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed
  4. Huang L, et al. 2018. Exp Neurol. 300:41. PubMed
  5. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  6. Billi AC, et al. 2019. JCI Insight. 4:8. PubMed
  7. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  8. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  9. Schneider C, et al. 2018. Cell. 174:271. PubMed
  10. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  11. Mak'Anyengo R, et al. 2018. JCI Insight. 3:e96322. PubMed
  12. Yeung F, et al. 2020. Cell Host & Microbe. 27(5):809-822. PubMed
  13. Jankovic D, et al. 2013. J Exp Med. 210:1899. PubMed
  14. Richardson M, et al. 2014. PLoS Negl Trop Dis. 8:2825. PubMed
  15. Wen T, et al. 2014. J Immunol. 192:5481. PubMed
  16. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  17. Ni P, et al. 2014. J Immunol . 193:1778. PubMed
  18. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  19. Keller S, et al. 2014. J Control Release. 191:24. PubMed
  20. Onishi M, et al. 2015. J Immunol. 194:2673. PubMed
  21. Wu Y, et al. 2015. J Immunol. 195: 2612-2623. PubMed
  22. Krishnamurthy B, et al. 2015. Diabetes. 64: 3229-3238. PubMed
  23. Saravia J, et al. 2015. PLoS Pathog. 11: e1005217. PubMed
  24. Katakam A, et al. 2015. Proc Natl Acad Sci U S A. 112: 14664 - 14669. PubMed
  25. Kitano M, et al. 2016. Proc Natl Acad Sci U S A. 113: 1044 - 1049. PubMed
  26. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  27. Kolb R, et al. 2016. Nat Commun. 7:13007. PubMed
  28. Boyd DF, et al. 2020. Nature. 587:466. PubMed
  29. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  30. Asadi Shahmirzadi A, et al. 2020. Cell Metabolism. 32(3):447-456.e6. PubMed
  31. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  32. Müller A, et al. 2020. J Clin Invest. 5765:130. PubMed
  33. Covarrubias AJ, et al. 2020. Nat Metab. 1265:2. PubMed
  34. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  35. Wawrzyniak M, et al. 2021. Pharmacol Res Perspect. 9:e00837. PubMed
  36. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  37. Zhang X, et al. 2021. Front Pharmacol. 12:706748. PubMed
  38. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  39. Wang Y, et al. 2021. Nature. 599:308. PubMed
  40. Menzel L, et al. 2021. Cell Rep. 37:109878. PubMed
  41. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  42. Zhao X, et al. 2022. Cell Biosci. 12:3. PubMed
  43. Darlyuk-Saadon I, et al. 2021. FEBS J. 288:3978. PubMed
  44. O'Leary CE, et al. 2021. Curr Protoc. 1:e77. PubMed
  45. Cohen K, et al. 2021. Cell Rep. 37:110026. PubMed
  46. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  47. Bettke JA, et al. 2022. Infect Immun. 90:e0007022. PubMed
  48. Kodali S, et al. 2022. J Immunol. 208:1085. PubMed
  49. Glorieux C, et al. 2022. J Adv Res. 40:109. PubMed
  50. Li J, et al. 2022. Elife. 11:. PubMed
  51. Kozlovski S, et al. 2023. Front Immunol. 13:1041552. PubMed
  52. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
RRID
AB_755987 (BioLegend Cat. No. 117321)
AB_755988 (BioLegend Cat. No. 117322)

Antigen Details

Structure
Integrin α-chain, associates with integrin β2 (CD18), 150 kD
Distribution

Dendritic cells, NK cells, intestinal intraepithelial lymphocytes (IEL), some activated T cells

Function
Cellular adhesion
Ligand/Receptor
iC3b, fibrinogen
Cell Type
Dendritic cells, Epithelial cells, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen Facts Book Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Lopez-Rodriguez C, et al. 1996. J. Immunol. 156:3780.

Gene ID
16411 View all products for this Gene ID
UniProt
View information about CD11c on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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