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Pacific Blue™ anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
Ter-119_PB_092409
C57BL/6 bone marrow cells stained with Ter-119 Pacific Blue™
  • Ter-119_PB_092409
    C57BL/6 bone marrow cells stained with Ter-119 Pacific Blue™
See Pacific Blue™ spectral data
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116231 25 µg 106€
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116232 100 µg 220€
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Sato K, et al. 2017. J Immunol. 10.4049/jimmunol.1602005. PubMed
  2. Eilken H, et al. 2017. Nat Commun. . 10.1038/s41467-017-01738-3. PubMed
  3. Kato K, et al. 2018. Nat Commun. 48:120. PubMed
  4. Greenwald AC, et al. 2019. J Exp Med. 216:215. PubMed
  5. Diéguez–Hurtado R, et al. 2019. Nat Commun. 10:2817. PubMed
  6. Schneider C, et al. 2018. Cell. 174:271. PubMed
  7. Cai S et al. 2017. Cell stem cell. 20(2):247-260 . PubMed
  8. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  9. Burr ML, et al. 2019. Cancer Cell. 36:385. PubMed
  10. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  11. Behling K, et al. 2016. J Nucl Med. 57: 1771 - 1777. PubMed
  12. Borrelli MR, et al. 2020. Stem Cells Transl Med. 1.347916667. PubMed
  13. Garcia-Fabiani MB, et al. 2020. Methods Enzymol. 632:369. PubMed
  14. Hemanthakumar KA, et al. 2021. eLife. 10:00. PubMed
  15. Zabala M, et al. 2020. Cell Stem Cell. 27(2):284-299.e8. PubMed
  16. Sperber HS, et al. 2020. FASEB J. 14615:34. PubMed
  17. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  18. Shue YT, et al. 2022. Nat Commun. 13:2690. PubMed
  19. Cuadrado M, et al. 2022. Sci Rep. 12:10489. PubMed
  20. Karaman S, et al. 2022. J Exp Med. 219:. PubMed
  21. Reinitz F, et al. 2022. Elife. 11:. PubMed
  22. Heitzeneder S, et al. 2022. Cancer Cell. 40:53. PubMed
  23. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  24. Wang D, et al. 2022. Cell Rep. 39:110587. PubMed
  25. Kim JW, et al. 2022. iScience. 25:105603. PubMed
  26. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
RRID
AB_2149212 (BioLegend Cat. No. 116231)
AB_2251160 (BioLegend Cat. No. 116232)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org
Go To Top Version: 2    Revision Date: 12.04.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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