- Clone
- L5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FLAG tag
- Isotype
- Rat IgG2a, λ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![L5_PE_DYKDDDDK_Antibody_FC_022814.jpg L5_PE_DYKDDDDK_Antibody_FC_022814.jpg](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/L5_PE_DYKDDDDK_Antibody_FC_022814.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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F-SIK2-FLAG transfected HEK293 cells were fixed and permeabilized with Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002), then stained with anti-DYKDDDDK (clone L5) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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637309 | 25 µg | 94€ | ||||
637310 | 100 µg | 288€ |
The DYKDDDDK tag, commonly referred to as Sigma®'s FLAG® Tag, is often used as a protein modification in order to simplify the labeling and detection of proteins.This unique amino acid sequence allows for specific antibody detection in western blotting, immunoprecipitation, and immunostaining techniques.Due to the short sequence, this modification is not likely to affect the structure or function of the modified proteins.
Product DetailsProduct Details
- Reactivity
- Epitope tag
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- DYKDDDDK-tagged mouse Langerin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
ICFC - Verified
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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The L5 clone has been demonstrated to have 2-8 fold better sensitivity in WB than another commonly used antibody clone, M2.
- Application References
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- Park SH, et al. 2008. J Immunol Methods. 331:27.
- Moon SH, et al. 2010. J. Biol Chem. 285:12935. PubMed
- Sasaki M, et al. 2011. J. Biol Chem. 286:39370. PubMed
- Sonder SU, et al. 2012. J Immunol. 188:5906. PubMed
- Jiang Y, et al. 2013. Int Immunol. 25:235. PubMed
- Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
- Toyo-Oak K, et al. 2014. J Neurosci. 34:12168. PubMed
- Product Citations
- RRID
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AB_2563147 (BioLegend Cat. No. 637309)
AB_2563148 (BioLegend Cat. No. 637310)
Antigen Details
- Biology Area
- Cell Biology
- Antigen References
-
1. Einhauer A. 2001. J. Biochem. Biophys. Methods. 49:455.
2. Knappik A and Pluckthun A. 1994. Biotechniques. 17:754. - Gene ID
- NA
- UniProt
- View information about DYKDDDDK on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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