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PE anti-mouse CD117 (c-Kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_111105
C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC
  • 2B8_111105
    C57BL/6 bone marrow cells stained with 2B8 PE and CD11b FITC
See PE spectral data
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105807 50 µg 79€
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105808 200 µg 202€
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Application References
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  2. Asada S, et al. 2018. Nat Commun. 9:2733. PubMed
  3. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  4. Mitroulis I et al. 2018. Cell. 172(1-2):147-161 . PubMed
  5. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  6. Kleppe M et al. 2018. Cancer cell. 33(1):29-43 . PubMed
  7. Johnson JL et al. 2018. Immunity. 48(2):243-257 . PubMed
  8. Zhu YP et al. 2018. Cell reports. 24(9):2329-2341 . PubMed
  9. Schmitt M et al. 2018. Cell reports. 24(9):2312-2328 . PubMed
  10. Sakashita A, et al. 2019. Biol Reprod. 100:697. PubMed
  11. Tamura M, et al. 2019. Sci Rep. 9:8171. PubMed
  12. Zhou J, et al. 2019. Nat Commun. 10:2427. PubMed
  13. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  14. Shimokawa C et al. 2017. Immunity. 46(5):863-874 . PubMed
  15. Sykes DB et al. 2016. Cell. 167(1):171-186 . PubMed
  16. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  17. Zhong C, et al. 2020. Immunity. 52(1):83-95.e4.. PubMed
  18. Wang C, et al. 2008. Am J Physiol Regul Integr Comp Physiol. 294:R811. PubMed
  19. Nakano N, et al. 2011. J Biol Chem. 286:12042. PubMed
  20. Takayama G, et al. 2013. Int Immunol. 25:215. PubMed
  21. Quick M, et al. 2012. Am J Physiol Regul Integr Comp Physiol. 303:580. PubMed
  22. Kakiuchi K, et al. 2014. Biol Reprod. 90:82. PubMed
  23. Sackstein C 2014. Proc Natl Acad Sci U S A. 111:10696. PubMed
  24. Ghazaryan S, et al. 2014. Mol Cell Biol. 34:2833. PubMed
  25. Svahn S, et al. 2015. Infect Immun . 83:514. PubMed
  26. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  27. Yamazaki S, et al. 2015. J Immunol. 195: 3427 - 3435. PubMed
  28. Svahn S, et al. 2016. Infect Immun . 84: 1205-1213. PubMed
  29. Liu Q, et al. 2016. Cell Death Dis. 1.93125. PubMed
  30. Shu Z, et al. 2020. Mar Drugs. 18:00. PubMed
  31. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  32. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  33. Pervolarakis N, et al. 2020. Cell Rep. 108273:33. PubMed
  34. Grainger N, et al. 2020. J Physiol. 598:3283. PubMed
  35. Zong L, et al. 2021. NPJ Aging Mech Dis. 7:25. PubMed
  36. Cho K, et al. 2021. iScience. 24:103117. PubMed
  37. Sharma GP, et al. 2021. PLoS One. 16:e0259042. PubMed
  38. Zhang J, et al. 2021. Stem Cell Res Ther. 12:579. PubMed
  39. Casellas Roman HL, et al. 2020. Leuk Res. 94:106372. PubMed
  40. Qian J, et al. 2021. Int J Mol Sci. 22:. PubMed
  41. Wu L, et al. 2022. Theranostics. 12:842. PubMed
  42. Groenen AG, et al. 2022. J Lipid Res. 63:100167. PubMed
  43. Nguyen N, et al. 2022. iScience. 25:103679. PubMed
  44. Klimovich B, et al. 2022. J Exp Clin Cancer Res. 41:80. PubMed
  45. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  46. Schönberger K, et al. 2022. STAR Protoc. 3:101408. PubMed
  47. Schönberger K, et al. 2022. Cell Stem Cell. 29:131. PubMed
  48. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  49. Yang T, et al. 2022. Nat Commun. 13:4464. PubMed
  50. Liu T, et al. 2022. Front Immunol. 13:901349. PubMed
  51. Roy IM, et al. 2021. Bio Protoc. 11:e4130. PubMed
  52. Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
  53. Kropp L, et al. 2020. Clin Exp Allergy. 50:213. PubMed
  54. Cela D, et al. 2022. J Leukoc Biol. 111:1235. PubMed
  55. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  56. Roy IM, et al. 2022. iScience. 25:105171. PubMed
  57. Chen Y, et al. 2023. Stem Cells Int. 2023:7482546. PubMed
  58. Sohn J, et al. 2022. Blood Adv. 6:2557. PubMed
  59. Li X, et al. 2022. Cell. 185:1709. PubMed
  60. Cacialli P, et al. 2022. EMBO J. 41:e108536. PubMed
  61. Schönberger K, et al. 2023. Anal Chem. 95:4325. PubMed
  62. Boers R, et al. 2023. Nat Biotechnol. . PubMed
  63. Vanneste D, et al. 2023. Nat Immunol. 24:827. PubMed
  64. Capellmann S, et al. 2023. Front Immunol. 14:1154416. PubMed
  65. van Os BW, et al. 2023. Front Cardiovasc Med. 10:1171764. PubMed
RRID
AB_313216 (BioLegend Cat. No. 105807)
AB_313217 (BioLegend Cat. No. 105808)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 06.28.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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